FastQCFastQC Report
Wed 25 May 2016
SRR1780159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90919
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5540660.93995754462763No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG1046311.50804562302709No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT9221.014089464248397No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG5990.6588281877275377No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC4840.532341974724755No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG3120.3431625952771148No Hit
CTTATACACATCTGACGCTGCCGACGACGATGCTGGTGTAGATCTCGGTG1340.14738393515106854No Hit
CTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT1100.12098681243744433No Hit
CTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGG1060.1165872919851736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCCG150.00221830170.029
CGCTGAC150.00221830170.016
CTTCACA150.00221830170.023
TTCACAC150.00221830170.024
TGGCACT150.00221830170.05
CTATACA252.8199865E-670.01
CATCTTC150.00221830170.020
GTTCCCT150.00221830170.036
CTCTCGG150.00221830170.010
ACTCTCG150.00221830170.09
GGCTGCC150.00221830170.016
TTCCCTC150.00221830170.037
TCTGTGG150.00221830170.042
TTGGGCG207.8894656E-570.02
GCGCTGG150.00221830170.015
GGCTCCA150.00221830170.015
TAACATT207.8894656E-570.059
CTTGTGC401.3278623E-1070.01
TTGGCAC150.00221830170.04
CGGCTGA150.00221830170.030
Produced by FastQC (version 0.11.5)