FastQCFastQC Report
Wed 25 May 2016
SRR1780159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90919
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1866620.530362190521235No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1614317.755364665251488No Hit
CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT86939.561257822897304No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG34233.7648896270306533No Hit
CATAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT26732.939979542229897No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT22192.440633970897172No Hit
CAAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT9961.095480592615405No Hit
CTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT7790.8568066080797193No Hit
CAAAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT6800.7479184768860194No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC5740.6313311849008458No Hit
ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT4820.5301422144986196TruSeq Adapter, Index 18 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC4670.5136440128026045No Hit
CTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT4570.5026452116719278No Hit
CTAAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT3610.3970567208174309No Hit
TCTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG2710.29806751064134007No Hit
CTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC2620.28816858962373104No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT2360.25957170668397145No Hit
TCTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCG2090.2298749436311442No Hit
CTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1780.19577866012604625No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC1780.19577866012604625No Hit
CATATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT1740.19137913967377557No Hit
CACTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC1600.1759808180908281No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1520.16718177718628668No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1440.1583827362817453No Hit
TATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCT1370.15068357549027156No Hit
TCATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1360.1495836953772039No Hit
CATATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1360.1495836953772039No Hit
CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT1310.14408429481186552No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT1260.13858489424652712No Hit
ATACCCATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT1200.13198561356812108TruSeq Adapter, Index 18 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCG1160.12758609311585037No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1160.12758609311585037No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC1090.11988693232437664No Hit
AAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT1070.1176871720982413RNA PCR Primer, Index 18 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT970.10668837096756453No Hit
TTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC910.10008909028915848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCAGG455.456968E-1270.0000126
ACCAGGG455.456968E-1270.0000127
TTTTGTG150.00221830170.037
ATCCCGT150.00221830170.044
CTCGAAA150.00221830170.039
ACACGTA150.00221830170.038
ACAAAGG150.00221830170.066
CAAGAGA301.0137046E-770.021
TCCTTGA150.00221830170.055
ACTCGAA252.8199865E-670.038
TAAACCC252.8199865E-670.03
AAACATC150.00221830170.06
GAAACAA150.00221830170.060
CAGTATT150.00221830170.047
TATGCCT207.8894656E-570.043
GCGCGTC150.00221830170.033
GCGAAAC150.00221830170.033
CCTTGAA150.00221830170.056
GAATGAC207.8894656E-570.042
GCAACAC207.8894656E-570.035
Produced by FastQC (version 0.11.5)