##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780159_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 90919 Sequences flagged as poor quality 0 Sequence length 76 %GC 61 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.249254831223396 32.0 27.0 32.0 22.0 32.0 2 22.485366095095635 27.0 13.0 32.0 8.0 32.0 3 27.74972228027145 32.0 27.0 32.0 13.0 32.0 4 27.42192501017389 32.0 27.0 32.0 13.0 32.0 5 24.903837481714493 27.0 22.0 32.0 8.0 32.0 6 31.334880498025715 37.0 27.0 37.0 13.0 37.0 7 34.1001770806982 37.0 32.0 37.0 27.0 37.0 8 31.197714449125044 37.0 27.0 37.0 13.0 37.0 9 35.121162793255536 37.0 37.0 37.0 32.0 37.0 10-11 33.4847996568374 37.0 34.5 37.0 24.5 37.0 12-13 34.82848469516823 37.0 37.0 37.0 29.5 37.0 14-15 34.89701822501347 37.0 37.0 37.0 29.5 37.0 16-17 31.351653669749997 37.0 29.5 37.0 17.5 37.0 18-19 33.344146987978306 37.0 34.5 37.0 20.0 37.0 20-21 34.93014111461851 37.0 37.0 37.0 29.5 37.0 22-23 33.41816891958777 37.0 34.5 37.0 20.0 37.0 24-25 33.14416128641978 37.0 34.5 37.0 22.0 37.0 26-27 32.54105302522025 37.0 32.0 37.0 20.0 37.0 28-29 33.03084063837042 37.0 34.5 37.0 17.5 37.0 30-31 33.73891595816056 37.0 37.0 37.0 24.5 37.0 32-33 33.284973437895275 37.0 37.0 37.0 17.5 37.0 34-35 33.75069017477095 37.0 37.0 37.0 24.5 37.0 36-37 32.89077090597125 37.0 34.5 37.0 20.0 37.0 38-39 31.873222318767255 37.0 29.5 37.0 13.0 37.0 40-41 32.93556352357594 37.0 34.5 37.0 17.5 37.0 42-43 32.969318844245976 37.0 34.5 37.0 17.5 37.0 44-45 33.637897469175854 37.0 37.0 37.0 24.5 37.0 46-47 32.99904860370219 37.0 32.0 37.0 17.5 37.0 48-49 31.834710016608188 37.0 29.5 37.0 15.0 37.0 50-51 32.605753472871456 37.0 32.0 37.0 13.0 37.0 52-53 31.536796489182677 37.0 29.5 37.0 13.0 37.0 54-55 31.985685060328425 37.0 29.5 37.0 13.0 37.0 56-57 31.628581484618174 37.0 29.5 37.0 13.0 37.0 58-59 31.816067048691693 37.0 29.5 37.0 13.0 37.0 60-61 32.30730100419054 37.0 32.0 37.0 17.5 37.0 62-63 33.767529339302016 37.0 34.5 37.0 27.0 37.0 64-65 33.950202927880866 37.0 37.0 37.0 27.0 37.0 66-67 33.77532748930366 37.0 37.0 37.0 24.5 37.0 68-69 32.891876285484884 37.0 32.0 37.0 17.5 37.0 70-71 28.910013308549367 34.5 20.0 37.0 13.0 37.0 72-73 31.478656826405924 37.0 29.5 37.0 13.0 37.0 74-75 33.11627382615295 37.0 32.0 37.0 13.0 37.0 76 33.7405602789296 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 1.3134465858193174 11101 2 -2.8193625045311883 11101 3 1.6235665054196602 11101 4 0.015701985105874172 11101 5 -1.5629582718145087 11101 6 -1.9438376215653363 11101 7 0.03646103158169467 11101 8 0.33374867392983987 11101 9 0.3429243980741248 11101 10-11 0.2586373930048609 11101 12-13 -0.6706641827836037 11101 14-15 0.4664292270391641 11101 16-17 -0.16201963821658083 11101 18-19 -0.5788042821743531 11101 20-21 0.9477783880877197 11101 22-23 0.053263305383040915 11101 24-25 0.031423978404653496 11101 26-27 0.35861236217532877 11101 28-29 0.6291460931555264 11101 30-31 0.495917750626127 11101 32-33 -0.43520149570736066 11101 34-35 -0.02478390235673089 11101 36-37 0.3315130434709559 11101 38-39 0.2478741426158102 11101 40-41 -0.34914429076784614 11101 42-43 -0.6160874196687232 11101 44-45 0.9818935948066496 11101 46-47 0.22946431503593345 11101 48-49 0.7887173792215378 11101 50-51 1.261634770100855 11101 52-53 -1.6209524883977373 11101 54-55 -0.8442008188504104 11101 56-57 -0.7622920058528635 11101 58-59 0.10854652289548383 11101 60-61 1.062059673264578 11101 62-63 0.0016776917103484834 11101 64-65 -0.1906489967250664 11101 66-67 0.5523758761233779 11101 68-69 0.030279991293241437 11101 70-71 -2.54804333790927 11101 72-73 -0.10744698223654936 11101 74-75 -0.7980395645927842 11101 76 -0.4613459154668078 11102 1 -1.4610791594381354 11102 2 0.26464833557721335 11102 3 0.6799350691052943 11102 4 -0.7436476083900594 11102 5 -0.053473176963560576 11102 6 -2.4782549657387776 11102 7 -0.5966012990416161 11102 8 -1.676007423631134 11102 9 0.3483444522746666 11102 10-11 -0.09176911106018082 11102 12-13 0.12743879824620308 11102 14-15 0.5672422351692461 11102 16-17 -0.7582256002762051 11102 18-19 -0.6492649867814038 11102 20-21 0.4431713420172656 11102 22-23 1.078737560125596 11102 24-25 -1.1303646394815274 11102 26-27 -0.22024942644255674 11102 28-29 1.0239970416650053 11102 30-31 0.7265410568591832 11102 32-33 0.5314651709593079 11102 34-35 0.5714220597028827 11102 36-37 -0.7413866855263365 11102 38-39 -0.15293886551426894 11102 40-41 0.1633218338933986 11102 42-43 0.8069478106835817 11102 44-45 0.39435971946790005 11102 46-47 0.19558897628255068 11102 48-49 -1.4408219161714122 11102 50-51 0.5535046888000466 11102 52-53 -0.9111963908367606 11102 54-55 0.053902162179397806 11102 56-57 -0.23166869961980296 11102 58-59 -0.3415889784595336 11102 60-61 -0.2981029283614447 11102 62-63 1.037721052143958 11102 64-65 0.13319924175731757 11102 66-67 0.44641381650277623 11102 68-69 -0.21091242063087634 11102 70-71 0.8516856593807027 11102 72-73 -0.022352131288037924 11102 74-75 -0.08394742367137553 11102 76 -1.3339746417540752 11103 1 -0.4657575220112342 11103 2 0.34067172739007745 11103 3 -0.25105908294148804 11103 4 1.512492742487133 11103 5 -1.2873913056185309 11103 6 0.4036163792904617 11103 7 0.5811764787361611 11103 8 -2.644428476262714 11103 9 -0.07855613252065297 11103 10-11 -0.6724708654461509 11103 12-13 -0.3520933654964864 11103 14-15 -0.31170513325180593 11103 16-17 -0.6675823254224014 11103 18-19 -0.50306615637205 11103 20-21 0.2314754355845139 11103 22-23 -0.3312039603422434 11103 24-25 -0.29527692018328366 11103 26-27 0.02185583671533209 11103 28-29 -0.3257105606741746 11103 30-31 -2.2582542647782375 11103 32-33 -1.6106400921985866 11103 34-35 -0.8525545484842496 11103 36-37 -0.7408018902046933 11103 38-39 -1.0090792163914628 11103 40-41 -1.6606547742937323 11103 42-43 -0.18369546417022775 11103 44-45 0.18792697092987964 11103 46-47 -0.9301420178694997 11103 48-49 0.8445582007876524 11103 50-51 -0.1938637322525949 11103 52-53 -0.9497928820648305 11103 54-55 -0.6308931594580258 11103 56-57 -1.4462885826607348 11103 58-59 -1.5673199726115783 11103 60-61 -1.3209099459052993 11103 62-63 -0.393273099902828 11103 64-65 -1.7030580681842054 11103 66-67 0.19144305626885938 11103 68-69 0.058093427322340574 11103 70-71 -2.171706153484795 11103 72-73 -2.943989558188626 11103 74-75 -1.516695961683073 11103 76 0.4847388085383244 11104 1 0.42897932009402595 11104 2 -0.3084510796274671 11104 3 0.2401689872339503 11104 4 -1.0664546259339218 11104 5 -2.2347597266711645 11104 6 -1.578839761060415 11104 7 -0.5767182581059416 11104 8 -1.0654811078416628 11104 9 -0.1838192904153928 11104 10-11 -0.6987866549198358 11104 12-13 0.21808207310000682 11104 14-15 0.5742597790288926 11104 16-17 1.0166282008933827 11104 18-19 0.15482858047005976 11104 20-21 0.7139315759353977 11104 22-23 -0.38383553928961334 11104 24-25 -1.0058032359727562 11104 26-27 -1.0921792510039623 11104 28-29 0.6479736498521476 11104 30-31 -0.8372016331992924 11104 32-33 1.0121669253452694 11104 34-35 -0.1332563028702154 11104 36-37 -0.42501241652048805 11104 38-39 0.34179797659099265 11104 40-41 0.5674154011448636 11104 42-43 -0.08720423610004957 11104 44-45 -1.066458993982394 11104 46-47 -0.09680868453617109 11104 48-49 0.43227749903326185 11104 50-51 0.6219257414316175 11104 52-53 1.1554702758299022 11104 54-55 0.07963315633144674 11104 56-57 0.6677465050585596 11104 58-59 -0.3480217269975441 11104 60-61 -0.06652398099301848 11104 62-63 0.36111286500945283 11104 64-65 -0.23814578748244486 11104 66-67 0.19144305626885938 11104 68-69 -0.7840118358355497 11104 70-71 -0.34714474997602096 11104 72-73 1.503378862864004 11104 74-75 0.7815496523520125 11104 76 0.06368617695937928 11105 1 -1.2035394768984524 11105 2 -1.445669124740249 11105 3 0.8648557040259313 11105 4 0.4730190582766056 11105 5 -0.7062509547413391 11105 6 -1.3488898863736978 11105 7 -0.22834733078764913 11105 8 -1.9545788522025624 11105 9 0.8324714364016543 11105 10-11 -0.12093577772684938 11105 12-13 -1.0402199319125245 11105 14-15 -0.7297815743545613 11105 16-17 0.3270918600412571 11105 18-19 -0.2464872090036252 11105 20-21 -0.15762230877638927 11105 22-23 0.009491528379562908 11105 24-25 0.25594488432800233 11105 26-27 0.46248866879553674 11105 28-29 -0.43770930754133985 11105 30-31 -0.2577843399662072 11105 32-33 -1.4530586385645066 11105 34-35 0.1289617422425664 11105 36-37 -0.6850374791771259 11105 38-39 -1.3505579131333185 11105 40-41 -0.5214003883288214 11105 42-43 -0.9283696496338791 11105 44-45 -0.42250535989718685 11105 46-47 0.16086675406032214 11105 48-49 -0.7400282653777595 11105 50-51 -1.5709000731047205 11105 52-53 -1.7076249622653314 11105 54-55 -1.0798279965507618 11105 56-57 -0.12313695358805887 11105 58-59 0.17845070408014863 11105 60-61 0.313801833543323 11105 62-63 -0.7892630748401714 11105 64-65 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2.0634858873639246 21612 6 2.280809361746602 21612 7 -0.015314749334010003 21612 8 -0.1181126867890292 21612 9 0.5179350955495252 21612 10-11 0.5292835205187671 21612 12-13 -0.001216172514034497 21612 14-15 0.11811942815170084 21612 16-17 0.6569790780863691 21612 18-19 0.8916706857332173 21612 20-21 0.5472649092687263 21612 22-23 1.2214276186051265 21612 24-25 -0.16369797281485887 21612 26-27 0.2586979419784896 21612 28-29 0.10411400072933219 21612 30-31 -0.6091314577606894 21612 32-33 0.08234236394176264 21612 34-35 -0.6595720923439004 21612 36-37 -0.574135223538029 21612 38-39 -0.20206167253181562 21612 40-41 0.0586434713203019 21612 42-43 0.46542734284732035 21612 44-45 1.3107339884737428 21612 46-47 -0.08803675471160943 21612 48-49 0.1077160955244949 21612 50-51 0.358767846694775 21612 52-53 0.9712597495141146 21612 54-55 0.0884050861560084 21612 56-57 0.08002720681295017 21612 58-59 0.301101080020004 21612 60-61 0.9334760190069815 21612 62-63 0.02777953167611713 21612 64-65 1.437292809008774 21612 66-67 0.36688165276009244 21612 68-69 -0.7050644674145019 21612 70-71 0.7405745482695885 21612 72-73 -0.32118254064476304 21612 74-75 0.7026022839309647 21612 76 -1.2345594370757098 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 1.0 16 2.0 17 2.0 18 14.0 19 49.0 20 100.0 21 309.0 22 561.0 23 1093.0 24 1457.0 25 2080.0 26 2513.0 27 3080.0 28 3526.0 29 4123.0 30 5000.0 31 6551.0 32 8538.0 33 12774.0 34 17509.0 35 17061.0 36 4575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.277862712964286 6.085636665603449 9.267589832708236 61.36891078872403 2 21.015871619169133 30.216571158309225 36.71150609896939 12.056051123552251 3 21.37750500450956 15.7559559163202 57.05580853076398 5.810730548406256 4 20.303786887229293 59.58050572487599 13.03577910007809 7.079928287816628 5 20.587555956400752 29.276608849635387 46.24335947381735 3.8924757201465043 6 22.297750646207998 62.722323049001815 9.661771984820987 5.318154319969202 7 21.92391029377798 15.31253093412818 5.591790494836063 57.17176827725778 8 21.518054532056006 61.42940419494275 4.419318294305921 12.633222978695322 9 21.20788834017092 11.761018049032655 2.960877264378183 64.07021634641823 10-11 21.678637028563884 36.68320153103311 30.919279798501965 10.718881641901033 12-13 22.269822589337764 6.074087924416239 35.56572333615636 36.09036615008964 14-15 22.9303005972349 4.930212606825856 8.135263256305063 64.00422353963418 16-17 53.13025880179061 30.633860909160905 3.000472948448619 13.235407340599876 18-19 53.93736079412654 9.842439574339373 3.169356834492809 33.05084279704128 20-21 23.97298694442306 5.081996062429194 3.09231293788977 67.85270405525797 22-23 23.088133393460115 35.6152179412444 3.0494176134801307 38.24723105181535 24-25 53.10590603362463 33.78301829721005 3.0544846588315524 10.05659101033377 26-27 53.905515189184136 36.25987998261893 3.1907462308932004 6.643858597303734 28-29 24.862908592894897 10.560850984253051 3.3121943975403303 61.26404602531173 30-31 50.75293418838205 4.139763064975636 3.2960807823035716 41.81122196433875 32-33 85.57946145723336 3.720630939809926 3.4258403731080604 7.274067229848645 34-35 56.09336244493453 5.219189458227236 4.278195447370881 34.40925264946736 36-37 26.889319064222 31.64245097284396 4.891166862811954 36.57706310012209 38-39 22.300069292447123 10.355371264532167 34.136429129224915 33.2081303137958 40-41 22.030840977584194 5.508260190501331 36.36408631954068 36.09681251237379 42-43 52.64249103587848 5.006709342484437 33.22444400448756 9.12635561714952 44-45 23.61332614744993 35.125221350872756 35.93143347375136 5.330019027925957 46-47 52.57018725767867 6.353562295487667 10.074518107075095 31.00173233975857 48-49 50.13418537379426 4.616196834545034 5.451555780419935 39.79806201124077 50-51 26.093005862361 5.338818068830498 35.25830684455394 33.309869224254555 52-53 21.476809027815968 6.718617670673896 62.103630704253234 9.7009425972569 54-55 21.36332389253994 6.686831468089202 37.41798883603267 34.53185580333819 56-57 51.78125584311311 4.744882807773953 10.707882840770356 32.76597850834259 58-59 23.295973250327222 8.80638384460552 58.754138389960076 9.143504515107185 60-61 51.2742111109889 34.384452094721674 10.37021964605858 3.9711171482308427 62-63 22.416903143491936 70.15827448910007 3.4069161222200224 4.017906245187971 64-65 20.628932201152715 72.25845835716486 3.0121210787980113 4.100488362884421 66-67 20.906111135553697 72.11931893176119 2.8949799815214043 4.079589951163712 68-69 22.62343404568902 70.33183383011252 2.947128762964837 4.097603361233626 70-71 47.556616328820155 44.74257305953651 3.029069831388379 4.671740780254952 72-73 87.07901625640687 5.369123825865065 3.1264436085263645 4.425416309201698 74-75 89.8978177658498 3.2282546526463984 2.672246909234898 4.201680672268908 76 90.10086122507342 3.099530340860345 2.5517779953144073 4.247830438751828 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.5 15 3.0 16 12.5 17 21.0 18 19.5 19 18.0 20 23.0 21 28.0 22 42.5 23 47.0 24 58.0 25 79.0 26 86.5 27 121.5 28 159.5 29 170.0 30 165.0 31 185.0 32 226.0 33 242.0 34 236.5 35 256.5 36 285.0 37 288.0 38 324.0 39 373.5 40 433.0 41 517.5 42 556.0 43 568.0 44 597.5 45 735.0 46 855.0 47 933.0 48 1248.5 49 1841.5 50 2197.0 51 3424.0 52 9133.0 53 24841.5 54 36068.0 55 19798.5 56 2604.5 57 1251.0 58 822.0 59 656.5 60 458.0 61 387.0 62 349.0 63 308.5 64 269.5 65 255.5 66 246.0 67 252.0 68 238.0 69 198.5 70 160.5 71 148.0 72 137.5 73 117.0 74 93.0 75 79.0 76 75.0 77 65.0 78 55.0 79 51.0 80 46.5 81 31.5 82 26.0 83 31.0 84 25.5 85 19.5 86 18.0 87 17.0 88 16.0 89 10.0 90 7.0 91 8.0 92 7.0 93 7.5 94 11.0 95 19.0 96 24.0 97 49.0 98 188.0 99 8148.0 100 15994.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.002199760226135351 3 0.0010998801130676756 4 0.0 5 0.0 6 0.004399520452270702 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0016498201696015136 20-21 0.0 22-23 0.0 24-25 0.0038495803957368643 26-27 0.01704814175254897 28-29 0.013748501413345944 30-31 0.008799040904541405 32-33 0.007699160791473729 34-35 0.00494946050880454 36-37 0.0 38-39 0.0 40-41 0.0010998801130676756 42-43 0.0010998801130676756 44-45 0.0 46-47 0.0016498201696015136 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0016498201696015136 56-57 0.0 58-59 0.002199760226135351 60-61 0.0 62-63 0.0010998801130676756 64-65 0.003299640339203027 66-67 0.003299640339203027 68-69 0.0 70-71 0.0 72-73 0.0010998801130676756 74-75 0.003299640339203027 76 0.002199760226135351 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 90919.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.09151002540723 #Duplication Level Percentage of deduplicated Percentage of total 1 80.23211255269065 16.119842937119856 2 9.487053155964308 3.8121844718925635 3 4.335687304976187 2.6133151486487973 4 1.9433951935183664 1.5618297605560993 5 1.0456013576394592 1.0503855079796303 6 0.6733453769091804 0.8117115234439446 7 0.3941533913614715 0.5543395769861085 8 0.2134997536541304 0.3431625952771148 9 0.16423057973394645 0.2969676305282724 >10 1.1496140581376253 4.777879211165983 >50 0.1751792850495429 2.273452193710886 >100 0.13138446378715718 5.460904761381009 >500 0.02189741063119286 3.3315368624819897 >1k 0.016423057973394647 9.145503140157722 >5k 0.005474352657798215 9.561257822897304 >10k+ 0.01094870531559643 38.28572685577272 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 18666 20.530362190521235 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 16143 17.755364665251488 No Hit CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 8693 9.561257822897304 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 3423 3.7648896270306533 No Hit CATAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 2673 2.939979542229897 No Hit CTTAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 2219 2.440633970897172 No Hit CAAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 996 1.095480592615405 No Hit CTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 779 0.8568066080797193 No Hit CAAAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 680 0.7479184768860194 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC 574 0.6313311849008458 No Hit ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT 482 0.5301422144986196 TruSeq Adapter, Index 18 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC 467 0.5136440128026045 No Hit CTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT 457 0.5026452116719278 No Hit CTAAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 361 0.3970567208174309 No Hit TCTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 271 0.29806751064134007 No Hit CTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC 262 0.28816858962373104 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT 236 0.25957170668397145 No Hit TCTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCG 209 0.2298749436311442 No Hit CTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 178 0.19577866012604625 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC 178 0.19577866012604625 No Hit CATATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT 174 0.19137913967377557 No Hit CACTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC 160 0.1759808180908281 No Hit CTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 152 0.16718177718628668 No Hit CCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 144 0.1583827362817453 No Hit TATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCT 137 0.15068357549027156 No Hit TCATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 136 0.1495836953772039 No Hit CATATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 136 0.1495836953772039 No Hit CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT 131 0.14408429481186552 No Hit CTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT 126 0.13858489424652712 No Hit ATACCCATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT 120 0.13198561356812108 TruSeq Adapter, Index 18 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCG 116 0.12758609311585037 No Hit CTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 116 0.12758609311585037 No Hit CTTTACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC 109 0.11988693232437664 No Hit AAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT 107 0.1176871720982413 RNA PCR Primer, Index 18 (95% over 21bp) CATATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT 97 0.10668837096756453 No Hit TTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC 91 0.10008909028915848 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.010998801130676757 0.0 6 0.0 0.0 0.0 0.010998801130676757 0.0 7 0.0 0.0 0.0 0.010998801130676757 0.0 8 0.0 0.0 0.0 0.010998801130676757 0.0 9 0.0 0.0 0.0 0.010998801130676757 0.0 10 0.0 0.0 0.0 0.010998801130676757 0.0 11 0.0 0.0 0.0 0.010998801130676757 0.0 12 0.0 0.0 0.0 0.012098681243744433 0.0 13 0.0 0.0 0.0 0.014298441469879783 0.0 14 0.0 0.0 0.0 0.014298441469879783 0.0 15 0.0 0.0 0.0 0.016498201696015135 0.0 16 0.0 0.0 0.0 0.016498201696015135 0.0 17 0.0 0.0 0.0 0.016498201696015135 0.0 18 0.0 0.0 0.0 0.01759808180908281 0.0 19 0.0 0.0 0.0 0.02529724260055654 0.0 20 0.0 0.0 0.0 0.02969676305282724 0.0 21 0.0 0.0 0.0 0.030796643165894918 0.0 22 0.0 0.0 0.0 0.036296043731233296 0.0 23 0.0 0.0 0.0 0.043995204522707027 0.0 24 0.0 0.0 0.0 0.05499400565338378 0.0 25 0.0 0.0 0.0 0.06599280678406054 0.0 26 0.0 0.0 0.0 0.07149220734939891 0.0 27 0.0 0.0 0.0 0.07589172780166961 0.0 28 0.0 0.0 0.0 0.08469076870621102 0.0 29 0.0 0.0 0.0 0.0945896897238201 0.0 30 0.0 0.0 0.0 0.10998801130676757 0.0 31 0.0 0.0 0.0 0.1396847743595948 0.0 32 0.0 0.0 0.0 0.15618297605560993 0.0 33 0.0 0.0 0.0 0.1858797391084372 0.0 34 0.0 0.0 0.0 0.22327566295273815 0.0 35 0.0 0.0 0.0 0.2650711072493098 0.0 36 0.0 0.0 0.0 0.35416139640779154 0.0 37 0.0 0.0 0.0 0.5246428139332813 0.0 38 0.0 0.0 0.0 0.6962241115718387 0.0 39 0.0 0.0 0.0 0.8392085262706365 0.0 40 0.0 0.0 0.0 0.9612952188211485 0.0 41 0.0 0.0 0.0 1.15927363917333 0.0 42 0.0 0.0 0.0 1.3891485828044743 0.0 43 0.0 0.0 0.0 1.5343327577294075 0.0 44 0.0 0.0 0.0 1.6938153741242206 0.0 45 0.0 0.0 0.0 1.9313894785468384 0.0 46 0.0 0.0 0.0 2.153565261386509 0.0 47 0.0 0.0 0.0 2.3339455999296077 0.0 48 0.0 0.0 0.0 2.4703307339499996 0.0 49 0.0 0.0 0.0 2.601216467405053 0.0 50 0.0 0.0 0.0 2.766198484365204 0.0 51 0.0 0.0 0.0 2.8860854166895806 0.0 52 0.0 0.0 0.0 3.0015728285616867 0.0 53 0.0 0.0 0.0 3.145657123373552 0.0 54 0.0 0.0 0.0 3.301840099429162 0.0 55 0.0 0.0 0.0 3.4327258328842154 0.0 56 0.0 0.0 0.0 3.5625116862262014 0.0 57 0.0 0.0 0.0 3.6637006566284276 0.0 58 0.0 0.0 0.0 3.7725887878221274 0.0 59 0.0 0.0 0.0 3.8506802758499323 0.0 60 0.0 0.0 0.0 3.9221724831993314 0.0 61 0.0 0.0 0.0 4.0079631320186095 0.0 62 0.0 0.0 0.0 4.079455339368009 0.0 63 0.0 0.0 0.0 4.183943950109438 0.0 64 0.0 0.0 0.0 4.278533639833258 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCAGG 45 5.456968E-12 70.00001 26 ACCAGGG 45 5.456968E-12 70.00001 27 TTTTGTG 15 0.002218301 70.0 37 ATCCCGT 15 0.002218301 70.0 44 CTCGAAA 15 0.002218301 70.0 39 ACACGTA 15 0.002218301 70.0 38 ACAAAGG 15 0.002218301 70.0 66 CAAGAGA 30 1.0137046E-7 70.0 21 TCCTTGA 15 0.002218301 70.0 55 ACTCGAA 25 2.8199865E-6 70.0 38 TAAACCC 25 2.8199865E-6 70.0 3 AAACATC 15 0.002218301 70.0 6 GAAACAA 15 0.002218301 70.0 60 CAGTATT 15 0.002218301 70.0 47 TATGCCT 20 7.8894656E-5 70.0 43 GCGCGTC 15 0.002218301 70.0 33 GCGAAAC 15 0.002218301 70.0 33 CCTTGAA 15 0.002218301 70.0 56 GAATGAC 20 7.8894656E-5 70.0 42 GCAACAC 20 7.8894656E-5 70.0 35 >>END_MODULE