FastQCFastQC Report
Wed 25 May 2016
SRR1780158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69746
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4466464.03808103690535No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG891212.777793708599777No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT7571.0853669027614488No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG4680.6710062225790726No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC4350.6236916812433688No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG2630.3770825567057609No Hit
CTTATACACATCTGACGCTGCCGACGACGATGCTGGTGTAGATCTCGGTG1120.1605826857454191No Hit
CTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGG910.130473432168153No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG720.10323172655062657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA207.876563E-570.034
CTATACA150.002216122870.01
GCTAGTG150.002216122870.032
GCAGTAT150.002216122870.054
AGTGTAG207.876563E-570.035
CGAATCA150.002216122870.056
CGGTGGA150.002216122870.046
GAATCAT150.002216122870.057
CTAGTGT150.002216122870.033
TGCTAGT150.002216122870.031
CGGCTGG150.002216122870.09
GGGCGCT150.002216122870.013
TGGGAAA150.002216122870.048
TCTTTAT401.3278623E-1070.01
AAAAAGG6300.059.44444370
GTGCGCG252.3754335E-456.0000044
CTTATAC12450.053.6947821
GTAGATA200.006924807652.538
TGCGCGT200.006924807652.55
GCGGCTG200.006924807652.58
Produced by FastQC (version 0.11.5)