FastQCFastQC Report
Wed 25 May 2016
SRR1780158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69746
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT1728924.788518337969204No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1312018.81111461589195No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT57558.251369254150775No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG28754.122100192125713No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT15022.1535285177644594No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT13281.9040518452671122No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC6920.9921715940699108No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT5880.8430591001634503No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC4400.6308605511427179No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT4320.6193903593037594No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT4130.5921486536862329No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT4110.5892811057264933No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT3000.430132193960944No Hit
CAAAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT2480.35557594700771367No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT2400.3441057551687552No Hit
TCTAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG2120.3039600837324004No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCTT2120.3039600837324004RNA PCR Primer, Index 26 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT2010.28818856995383246No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCG1980.28388724801422305No Hit
CTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC1710.2451753505577381No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC1700.24374157657786827No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT1560.22366874085969088No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT1550.22223496687982106No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT1320.18925816534281537No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT1290.18495684340320592No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG1230.17635419952398704No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC1230.17635419952398704No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG1200.1720528775843776No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC1120.1605826857454191No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT1070.15341381584607003No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT1020.14624494594672094No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATTCCGT970.1390760760473719No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCAT950.13620852808763226No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGAGTATCTCGTATGCCGT860.12330456226880394No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG860.12330456226880394No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT830.11900324032919452No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGA820.1175694663493247No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGA780.11183437042984544No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT760.1089668224701058No Hit
TATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCT730.10466550053049638No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC730.10466550053049638No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACG730.10466550053049638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACC207.8493766E-570.048822
ACATATA150.002209997570.048812
CACTTAT252.8014292E-670.048811
CCGTCTA353.6470738E-969.9985747
ACTGCTT150.002216302869.99856653
CTCGAAT900.069.99856639
TTATGAT150.002216302869.99856652
TAGAAAG150.002216302869.99856640
AAGTATG150.002216302869.99856640
CGTCAAC301.01037585E-769.99856648
CGTCAAA150.002216302869.99856648
CAAGAGA207.8773635E-569.99856621
GGATGCC150.002216302869.99856642
GTATGAA207.8773635E-569.99856642
CAAGAAA455.456968E-1269.99856657
AATCTCG500.069.99856636
CAGTCAT150.002216302869.99856647
TAAACCC252.8134164E-669.9985663
TTCCGTA150.002216302869.99856645
TGCAAGA207.8773635E-569.99856655
Produced by FastQC (version 0.11.5)