FastQCFastQC Report
Wed 25 May 2016
SRR1780157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328243
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5075115.461411210597026No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG84412.5715704523782685No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT8200.24981492369981995No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG6150.18736119277486496No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC4150.1264307235797869No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGA150.002223618669.9989349
TGGTCGC11700.054.4436249
GCCGTAT11550.053.93857254
TCGGTGG11850.053.459145
CGGTGGT12000.053.37418746
CGTATCA11750.053.31833656
TCGCCGT11700.053.24705552
CTCGGTG11950.053.01174544
TCTCGGT11900.052.9403743
GTGGTCG12300.052.92602548
CGCCGTA12000.052.79086353
AATCCGC200.00694805452.499262
ACGTATC200.00694805452.499255
GATCTTA200.00694805452.49921
GTCGCCG12150.052.42718551
CCGTATC11900.052.35214255
GGTGGTC12400.052.21694647
GTAGATC12200.052.2123238
ATCTCGG12000.052.2075442
TAGATCT12350.051.0113739