FastQCFastQC Report
Wed 25 May 2016
SRR1780157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328243
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT200826.118028411877786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126653.858421961778317No Hit
CATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT80862.463418869557005No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG35291.075118128947152No Hit
CATAAACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT22910.6979585246296189No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT20210.6157023912162636No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC9930.30251977955356246No Hit
CAAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT7680.23397300170909965No Hit
CTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT6910.2105147710689946No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC4880.14867034483599043No Hit
CAAAAACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT4650.14166334087855642No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCTT3870.11790045789247601RNA PCR Primer, Index 39 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA150.002220548570.02331526
TACGGTT150.002224578869.9913243
ACGCTAT150.002224578869.9913234
AGGGGGG43450.065.7325970
TAAGCCG1250.061.59235843
AAGCCGT1350.059.62223444
CATATAC12950.057.037331
GCCGTCA5350.056.9088346
CCGTCTT47400.056.55416547
CGTCTTC46650.056.42174548
TATGCCG53600.056.4109143
CGTATGC54100.056.4070541
ATGCCGT53300.056.40013544
GCCGTCT48100.056.38593346
TCGTATG53900.056.3566440
GTATGCC53900.056.29171442
CTCGTAT54000.056.2522839
TGCCGTC52450.056.24659745
ATCTCGT54700.056.23616837
TCTCGTA54850.056.14618338