FastQCFastQC Report
Wed 25 May 2016
SRR1780156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198217
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6355632.06384921575849No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG93774.730673958338588No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT9130.4606063052109557No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG6230.3143020023509588No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC5120.2583027691873048No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG3170.15992573795385864No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTGC150.002220244170.0153928
CGTTTAC150.002220244170.0153970
CCGTTTA150.002220244170.0153969
TGGACGC150.002222467769.9977349
TGCGGGT207.913737E-569.997735
GACTTTC150.002222467769.9977363
GTGGACG150.002222467769.9977348
TACATGA150.002222467769.9977339
GTTGTGC150.002222467769.997731
CGCGTCT150.002222467769.997737
GCCGTTT150.002222467769.9977368
CTTTATA800.056.8731542
CGCCATT252.383603E-456.01231433
ATTGGCG252.3865775E-455.99818837
GGTTCAT252.3865775E-455.9981881
TGTCGGA252.3865775E-455.99818835
GCCGTAT13300.054.9982254
TCGCCGT13400.054.5877852
CGTATCA13450.054.38485356
GTATCAT13550.054.2417857
Produced by FastQC (version 0.11.5)