FastQCFastQC Report
Wed 25 May 2016
SRR1780156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198217
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT2493512.579647557979387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142307.179000792061226No Hit
CATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT104475.27048638613237No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG46612.351463295277398No Hit
CATAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT30371.5321591992614154No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT26351.3293511656416956No Hit
CAAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT10520.5307314710645404No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC10300.5196325239510233No Hit
CTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT9210.4646422859795073No Hit
CAAAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT6460.32590544706054475No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC5500.2774736778379251No Hit
ATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT5210.2628432475519254Illumina PCR Primer Index 3 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT4030.20331253121578874No Hit
TCTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG3950.1992765504472371No Hit
CTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC2770.13974583411110045No Hit
CACTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC2290.11552994949979065No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2150.10846698315482527No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGTC150.00222281769.9949612
CCAGCGT150.00222281769.9949619
CACGAAT150.00222281769.9949639
GACAACT207.9152895E-569.9949534
ATATGCA207.9152895E-569.9949515
AGGGGGG51450.065.3694470
CGTCTTA650.064.61072548
CATATAC14550.062.778981
ATCGTAT900.062.2177439
ATATACA14950.060.162892
ATATCGT1000.059.49571637
ACCGAGC950.058.9431213
TAAACAC9450.058.8846443
CATAAAC5550.058.6444171
CTTAAAC4250.058.4663731
CGCAGCC601.8189894E-1258.34384530
AGCCGAC308.358209E-658.3291345
TACCCGC308.358209E-658.3291311
CTACCCG308.358209E-658.3291310
CACGTAT601.8189894E-1258.3291339
Produced by FastQC (version 0.11.5)