FastQCFastQC Report
Wed 25 May 2016
SRR1780155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123386
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5705846.243496020618224No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG94157.630525343231809No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT8930.7237449953803511No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG5220.4230625840857148No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC3990.32337542346781645No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG3010.2439498808616861No Hit
CTTATACACATCTGACGCTGCCGACGACGATGCTGGTGTAGATCTCGGTG1240.10049762533836902No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG150.002218705170.0117652
TCTCGGA150.002218705170.01176543
ACAATGG150.002218705170.01176537
CGACTCC150.002218705170.01176529
AACGTTG150.002222277569.9833845
ACGTTGT150.002222277569.9833846
GTGGATA150.002222277569.9833851
GTTCAGC150.002222277569.9833814
TGGCGCT308.34775E-658.3194913
TCTTTAT553.45608E-1157.282351
AAAAAGG6050.057.2591370
GTGGCGC252.3854467E-455.98670612
TAGTGTA252.3854467E-455.98670634
GCCGTAT12100.053.81068854
CGCCGTA12250.053.7233153
CGTATCA12250.053.4375556
CCGTATC12200.053.3696255
TCGCCGT12200.053.3479952
TCGGTGG12550.053.25428845
TGGTCGC12300.053.1987549