FastQCFastQC Report
Wed 25 May 2016
SRR1780155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123386
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT2119017.173747426774515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1392511.285721232554746No Hit
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT98687.997665861605044No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG39523.2029565753002776No Hit
CATAAACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT27672.242555881542476No Hit
CTTAAACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT23961.9418734702478402No Hit
CAAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT9060.7342810367464705No Hit
CTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT7570.6135217933963335No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC6340.5138346327784352No Hit
CAAAAACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT5730.46439628482972134No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC4740.38416027750312026No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT4210.3412056473181722TruSeq Adapter, Index 3 (95% over 23bp)
TCTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG3150.25529638694827617No Hit
CTAAAACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT2960.2398975572593325No Hit
CTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC2340.189648744590148TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT1730.1402103966414342No Hit
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT1700.13777900248002206No Hit
CACTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC1600.12967435527531487No Hit
CTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG1580.12805342583437343No Hit
TATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCT1550.12562203167296127RNA PCR Primer, Index 3 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG1530.12400110223201985No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT1480.11994877862966626No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC1400.1134650608659005No Hit
TCATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG1310.10617087838166404No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGAC207.8920144E-570.0154147
GAATACC207.8920144E-570.0154142
CCGTATA150.002218246570.0154147
ATATCGA207.8920144E-570.0154137
CCGACTA150.002218246570.0154147
AACTCGA207.8920144E-570.0154137
TATCGAA207.8920144E-570.0154138
GTCGCAC150.00222181869.9870423
TCGCACA150.00222181869.9870424
TTAGCTA150.00222181869.9870417
GCAGTCG150.00222181869.9870420
GTGGACC150.00222181869.9870448
TTCCCCA150.00222181869.9870421
TGATTAC150.00222181869.9870425
CAGTCGC150.00222181869.9870421
CGCACAT150.00222181869.9870425
GTCCATC150.00222181869.9870433
CGACTAA150.00222181869.9870448
TCACCGA1350.069.9870311
ACCGAGC1300.069.9870313
Produced by FastQC (version 0.11.5)