FastQCFastQC Report
Wed 25 May 2016
SRR1780154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103815
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3534434.04517651591774No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG61315.905697635216491No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT5750.5538698646631026No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG3560.34291769012185136No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC3290.31690988778114915No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG1700.16375282955256948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGAG150.002219191570.03
CTCTTGA150.002219191570.02
GAAGTCT150.002219191570.065
TCGAGTA150.002219191570.070
AAAAAGG5200.055.86538770
TCGCCGT9150.053.55191452
CGCCGTA9150.053.55191453
GCCGTAT9200.053.26086854
CGTATCA9250.052.97297356
TGGTCGC9450.052.96296349
CGGTGGT9750.052.76923446
TCGGTGG9700.052.68041245
GTCGAGT200.006934322452.569
TTAGAGG200.006934322452.54
TGAGTAT200.006934322452.548
CCCAAGG200.006934322452.563
CTTGAGG200.006934322452.54
GCCAAAC200.006934322452.57
CTCGGTG9550.052.4083844
GTCGCCG9350.052.40641851
Produced by FastQC (version 0.11.5)