FastQCFastQC Report
Wed 25 May 2016
SRR1780154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103815
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT1324812.761161681837885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91888.850358811347107No Hit
CATATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT58555.639840100178201No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG25072.4148726099311273No Hit
CATAAACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT16121.5527621249337764No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT14801.4256128690458991No Hit
CAAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT5240.5047440157973317No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC4910.47295670182536237No Hit
CTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT4560.43924288397630395No Hit
CAAAAACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT3470.33424842267495064No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT2980.28704907768626886TruSeq Adapter, Index 11 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC2860.27549005442373453No Hit
CTAAAACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT2040.19650339546308337No Hit
TCTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG1650.15893656985984686No Hit
CACTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC1480.14256128690458988No Hit
CTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC1460.14063478302750085No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT1170.11270047680970957Illumina PCR Primer Index 6 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC1120.10788421711698695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTT252.822815E-670.0000147
TCTGCAA150.002219191570.053
GTCTAGC150.002219191570.028
AATGACG150.002219191570.043
GAGTTAG150.002219191570.046
AACTCGT207.89474E-570.037
CGACTTC150.002219191570.048
CAACTCG207.89474E-570.036
CAGAGTT150.002219191570.044
TAGCTAG150.002219191570.031
CGCCCAA150.002219191570.030
GACTAGC150.002219191570.026
CTAGCTA150.002219191570.030
AGACACG455.456968E-1269.9999925
AGCCGTC700.065.045
CATATAC7800.064.615391
AGGGGGG27900.064.3548470
TCGCCAA600.064.16666429
CTTTATA600.064.1666642
ATATACA8000.062.5625042
Produced by FastQC (version 0.11.5)