FastQCFastQC Report
Wed 25 May 2016
SRR1780153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100996
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3626335.905382391381835No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG61556.094300764386708No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT5540.5485365757059686No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC3860.3821933541922452No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG3670.3633807279496218No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG2080.20594875044556218No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC150.002219013970.000012
TGTGCGC150.002219013970.000013
GCGCTGC150.002219013970.0000115
TTTCGGT207.8936915E-570.027
CGCGTCT207.8936915E-570.07
TGCCGGC207.8936915E-570.019
CGTATCA9650.052.95336556
TCGCCGT9700.052.68041252
CTTGTGC200.006933774352.51
GGCACAT200.006933774352.569
GAACTCG200.006933774352.541
GCCATTT200.006933774352.520
GCACATG200.006933774352.570
TGCGCGT200.006933774352.55
TCGCTAC200.006933774352.542
TTTGGGG200.006933774352.530
GTATCAT9800.052.557
AAGGCGG200.006933774352.524
TAATGGC200.006933774352.565
TCGGTGG9900.052.32323545
Produced by FastQC (version 0.11.5)