FastQCFastQC Report
Wed 25 May 2016
SRR1780153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100996
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT1490414.75702008000317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92789.18650243574003No Hit
CATATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT57025.645768149233633No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG26892.662481682442869No Hit
CATAAACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT15471.5317438314388687No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT14611.4465919442354152No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC7770.7693373995009702No Hit
CAAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT5860.5802209988514396No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT5200.5148718761139055No Hit
CAAAAACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT3550.3514990692700701No Hit
ATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT3360.33268644302744665RNA PCR Primer, Index 30 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC3360.33268644302744665No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC2000.19802764465919445No Hit
CTAAAACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT1900.1881262624262347No Hit
TCTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG1850.18317557130975484No Hit
CTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC1840.18218543308645888No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC1200.11881658679551667No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG1130.11188561923244486No Hit
CTTTACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC1030.10198423699948514No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG1020.10099409877618916No Hit
TATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCT1020.10099409877618916No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCCT150.002219013970.0000137
CCAATGT150.002219013970.0000114
GTTGTCG150.002219013970.0000155
GTCGTGC150.002219013970.0000158
ACAGACG150.002219013970.0000122
GACGAGG150.002219013970.0000125
ACGAGGT150.002219013970.0000126
CGATCCC150.002219013970.0000136
AGGGGGG28750.065.00870570
ACTCGTA501.4551915E-1162.99999638
TGGAACT453.8016879E-1062.22222534
AACTCGT453.8016879E-1062.22222537
CATATAC7850.061.974521
CGTCTTA352.9486546E-760.048
ACTCCGA700.060.011
ATATACA8100.059.6296272
TAAACAC5050.059.6039623
AACACAT6250.057.685
TATGACG850.057.6470643
CTGGATA850.057.6470633
Produced by FastQC (version 0.11.5)