FastQCFastQC Report
Wed 25 May 2016
SRR1780152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92014
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6304268.51348707805333No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG1094711.897102614819485No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT10651.1574325646097334No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG5520.5999087095441998No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC5270.5727389310322342No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG3360.3651618232008173No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2030.22061860151716042No Hit
CTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGG1300.14128284826222096No Hit
CTTATACACATCTGACGCTGCCGACGACGATGCTGGTGTAGATCTCGGTG1100.1195470254526485No Hit
CTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT990.10759232290738366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATG150.002218386270.0000129
CAGTAAA150.002218386270.0000161
ATGCGCG150.002218386270.000014
TCAGTAA150.002218386270.0000160
TTGATGG150.002218386270.0000130
GCGTCTG500.070.000018
GACGACC150.002218386270.0000123
CTTGTGC301.0138319E-770.000011
GGGGTAT150.002218386270.0000154
CGTTGAT150.002218386270.0000128
AAAAATG150.002218386270.0000170
TTGTGCG301.0138319E-770.000012
TTTATAC500.070.000013
TGCGCGT401.3278623E-1070.05
GATGCTG401.3278623E-1070.029
ATGGTGT207.889973E-570.033
TTGGGCG207.889973E-570.02
CGCGGCC207.889973E-570.016
TGGCGCT401.3278623E-1070.013
CGATGCT353.6634447E-970.028
Produced by FastQC (version 0.11.5)