##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780152_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 92014 Sequences flagged as poor quality 0 Sequence length 76 %GC 87 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.827982698285044 32.0 27.0 32.0 22.0 32.0 2 29.48984937074793 32.0 32.0 32.0 22.0 32.0 3 29.279522681331102 32.0 32.0 32.0 22.0 32.0 4 28.737952920207796 32.0 32.0 32.0 13.0 32.0 5 29.543667268024432 32.0 32.0 32.0 22.0 32.0 6 33.65374834264351 37.0 37.0 37.0 22.0 37.0 7 33.30273654009172 37.0 37.0 37.0 13.0 37.0 8 33.65537852935423 37.0 37.0 37.0 22.0 37.0 9 33.70025213554459 37.0 37.0 37.0 22.0 37.0 10-11 33.55950181494121 37.0 37.0 37.0 22.0 37.0 12-13 33.243990044993154 37.0 37.0 37.0 13.0 37.0 14-15 33.54824265872584 37.0 34.5 37.0 20.0 37.0 16-17 34.00480361684092 37.0 37.0 37.0 24.5 37.0 18-19 33.603207120655554 37.0 37.0 37.0 22.0 37.0 20-21 33.99368574347382 37.0 37.0 37.0 24.5 37.0 22-23 34.13337100875954 37.0 37.0 37.0 24.5 37.0 24-25 33.42321820592518 37.0 34.5 37.0 17.5 37.0 26-27 33.78955919751343 37.0 34.5 37.0 20.0 37.0 28-29 34.000010867911406 37.0 37.0 37.0 22.0 37.0 30-31 33.903873323624666 37.0 37.0 37.0 22.0 37.0 32-33 33.994538874519094 37.0 37.0 37.0 22.0 37.0 34-35 34.008808442193576 37.0 37.0 37.0 22.0 37.0 36-37 34.65631316973504 37.0 37.0 37.0 29.5 37.0 38-39 34.818848218749324 37.0 37.0 37.0 29.5 37.0 40-41 34.30832264655379 37.0 37.0 37.0 27.0 37.0 42-43 34.18976460103897 37.0 37.0 37.0 27.0 37.0 44-45 34.02596344034603 37.0 37.0 37.0 24.5 37.0 46-47 34.27516464885778 37.0 37.0 37.0 27.0 37.0 48-49 34.69407372791097 37.0 37.0 37.0 29.5 37.0 50-51 34.94330210620123 37.0 37.0 37.0 32.0 37.0 52-53 34.28691829504206 37.0 37.0 37.0 27.0 37.0 54-55 34.3515062925207 37.0 37.0 37.0 27.0 37.0 56-57 34.25224965766079 37.0 37.0 37.0 24.5 37.0 58-59 33.9661573238855 37.0 37.0 37.0 22.0 37.0 60-61 33.85897254765579 37.0 37.0 37.0 22.0 37.0 62-63 33.43206468580868 37.0 37.0 37.0 17.5 37.0 64-65 33.035510900515135 37.0 34.5 37.0 17.5 37.0 66-67 33.37044362814355 37.0 37.0 37.0 22.0 37.0 68-69 33.63228421761906 37.0 37.0 37.0 22.0 37.0 70-71 33.644619297063485 37.0 37.0 37.0 22.0 37.0 72-73 33.768975373312756 37.0 37.0 37.0 22.0 37.0 74-75 32.6550959636577 37.0 34.5 37.0 17.5 37.0 76 32.781728867346274 37.0 37.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -0.8094620644797175 11101 2 1.3074947293380212 11101 3 -0.29852566469964614 11101 4 0.38903341393334756 11101 5 0.8286639564287768 11101 6 0.9779376777302744 11101 7 0.351591649069519 11101 8 0.828544406330785 11101 9 1.773102760111179 11101 10-11 0.7579348545543283 11101 12-13 0.7131257349159981 11101 14-15 0.6259065876390864 11101 16-17 0.6100398580331188 11101 18-19 0.09642429062363078 11101 20-21 1.4004729064710588 11101 22-23 0.3342659636561649 11101 24-25 1.4960475861912101 11101 26-27 0.3984403500975304 11101 28-29 0.31335604294553576 11101 30-31 -0.4634867908864564 11101 32-33 -0.5459974553182647 11101 34-35 0.20610260739104547 11101 36-37 0.38283707937848277 11101 38-39 0.5424042567859928 11101 40-41 0.009297557658861422 11101 42-43 0.2907001909839053 11101 44-45 0.2888772049455497 11101 46-47 0.12994452138781298 11101 48-49 0.32830030120489084 11101 50-51 -0.36448128852409667 11101 52-53 -0.266320884883136 11101 54-55 0.5160564598049788 11101 56-57 -0.9768826606996868 11101 58-59 0.10171679751388041 11101 60-61 0.9978298788660354 11101 62-63 -0.6703469067033936 11101 64-65 2.1483953615045905 11101 66-67 1.044766310210406 11101 68-69 0.6823416681406158 11101 70-71 -0.06877843215581692 11101 72-73 0.02870098549860245 11101 74-75 -0.186324340179695 11101 76 -0.3722817780090608 11102 1 1.317522062504409 11102 2 1.2065663076548852 11102 3 1.3354964976244048 11102 4 0.4534239500219961 11102 5 1.4701733903910394 11102 6 1.894080235382262 11102 7 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0.2301592111646329 21611 36-37 0.665069783781 21611 38-39 0.663787904584737 21611 40-41 -0.11491627881913047 21611 42-43 0.8089391846946015 21611 44-45 -0.26473914725571035 21611 46-47 0.5475545842808884 21611 48-49 0.5570738861105511 21611 50-51 0.5793866360042088 21611 52-53 1.0563206245508283 21611 54-55 -0.25422655906294267 21611 56-57 0.5055072764072435 21611 58-59 0.7617796905956453 21611 60-61 0.21418208012389783 21611 62-63 1.2870430304035239 21611 64-65 0.8729236633913828 21611 66-67 0.8707097064368128 21611 68-69 0.15372531593936145 21611 70-71 0.48232219677500865 21611 72-73 0.3722544446181004 21611 74-75 -0.10204761061994816 21611 76 1.4469006119280508 21612 1 -0.5453111210834898 21612 2 0.6659852953757586 21612 3 1.2109082975645045 21612 4 1.4456371875182548 21612 5 0.3758337677495298 21612 6 0.3364282437680046 21612 7 -0.38425740753425686 21612 8 0.22477082142512472 21612 9 1.4146121940734417 21612 10-11 0.18246315644112343 21612 12-13 0.01501252736883174 21612 14-15 -0.14767831802129194 21612 16-17 -0.3050544815895293 21612 18-19 0.06812240383118251 21612 20-21 0.7023596989238854 21612 22-23 0.34369992592031906 21612 24-25 -0.6265939232427584 21612 26-27 -0.8468426687703996 21612 28-29 -0.6489081079978618 21612 30-31 -0.7559396210751288 21612 32-33 -0.4327899081484574 21612 34-35 -0.2938973926089474 21612 36-37 -0.39074782628190263 21612 38-39 -0.7689164979309808 21612 40-41 -0.3208911215864205 21612 42-43 -0.482884714676473 21612 44-45 0.4681224879644219 21612 46-47 -0.10590453521596999 21612 48-49 -0.0018883780403911032 21612 50-51 -0.27957562814673054 21612 52-53 0.24311307738101817 21612 54-55 0.07266023338988958 21612 56-57 -1.2315996418317567 21612 58-59 -0.511490749655934 21612 60-61 1.2053770486773558 21612 62-63 0.2824832819758498 21612 64-65 0.5257538520706291 21612 66-67 0.6013700837953024 21612 68-69 -0.09124323751976249 21612 70-71 1.1387687376555036 21612 72-73 1.1985123062533205 21612 74-75 0.08726056548067618 21612 76 -0.4666214006505669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 2.0 11 6.0 12 30.0 13 80.0 14 157.0 15 279.0 16 435.0 17 707.0 18 895.0 19 853.0 20 853.0 21 785.0 22 884.0 23 938.0 24 1018.0 25 1178.0 26 1303.0 27 1348.0 28 1477.0 29 1768.0 30 2038.0 31 2353.0 32 3182.0 33 4490.0 34 8127.0 35 15332.0 36 41495.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 77.46973286673767 1.9594844262829572 2.9267285413089312 17.644054165670443 2 76.03273450490691 1.4954408616173802 18.98753437014335 3.4842902633323556 3 75.55480687721435 2.2257482557002195 20.205620883778554 2.013823983306888 4 76.80277358141961 15.727467368032084 5.043962134962124 2.42579691558618 5 76.14819483991566 4.4623644228052255 18.437411698219837 0.9520290390592736 6 77.97630692316052 17.343766981849797 2.8377350288012173 1.8421910661884577 7 76.80219099475075 4.424374816602001 2.3844456761544564 16.3889885124928 8 77.78924946203838 17.257156519660054 1.7725563501206336 3.181037668180929 9 76.29056447931836 3.189731997304758 1.069402482230965 19.450301041145913 10-11 77.60491666983648 10.025159351616884 9.280159976525184 3.0897640020214423 12-13 76.84185930248987 2.6349537565344026 10.384945605512264 10.138241335463468 14-15 86.0732392529357 8.413982730794938 2.7582909029653258 2.7544871133040263 16-17 85.47612319864368 2.7876192753276676 1.5003151694307388 10.235942356597908 18-19 78.15556666521758 1.4405729687432074 8.888514541581532 11.515345824457679 20-21 85.68385473554936 1.644903082766828 2.8164957641164423 9.85474641756737 22-23 85.4835729733842 1.436753502222512 1.0243117820308 12.055361742362493 24-25 79.19141421002581 9.579676674364897 1.0112756418964814 10.21763347371281 26-27 86.14745902708343 9.124896752597488 1.5563187410337782 3.1713254792853105 28-29 85.63556927357301 2.962657044733296 1.9644176846498285 9.437355997043865 30-31 79.4540870113356 1.915531838584517 16.253491430372456 2.3768897197074264 32-33 86.21896413996228 1.6460077925888894 3.6919699382135733 8.44305812923525 34-35 85.95555990283823 1.6574015204621162 9.604777663661608 2.7822609130380442 36-37 87.33052574378482 1.5595707105012906 9.92691210433365 1.1829914413802471 38-39 86.76857366430465 1.7057557709863933 10.374733730382992 1.1509368343259574 40-41 85.92489145614502 9.319284670184267 3.2886478614551127 1.4671760122155988 42-43 79.55244481638066 9.742856832335264 8.94566953951158 1.7590288117725055 44-45 78.37751187332225 2.0372121330681536 10.270287894101921 9.314988099507678 46-47 85.45904089118326 1.2438527373998098 3.365847031653308 9.931259339763619 48-49 87.9272494104094 1.0846293458533032 9.010139871539891 1.9779813721974067 50-51 94.09536747724493 1.1139790789294934 3.193587827740796 1.597065616084771 52-53 80.11302812117918 1.3470995788615678 9.386224697731286 9.15364760222796 54-55 86.10631106474085 1.3112277612945997 2.2888070163998564 10.293654157564692 56-57 85.65955712539058 1.682923515826654 1.8356201603043065 10.821899198478468 58-59 79.41670334686425 8.979855778897203 9.297207414290606 2.3062334599479413 60-61 77.61717157994838 3.239777204184214 10.486075261513381 8.656975954354028 62-63 77.79866865914957 10.202961554136666 9.205814427387583 2.7925553593261787 64-65 77.60008259837086 10.467169864636485 10.669318509099405 1.2634290278932525 66-67 77.72289333398543 19.215532841003572 1.8470517272297486 1.2145220977812556 68-69 77.7611737535661 19.89838337182448 1.0792011954897434 1.261241679119685 70-71 77.89751114009347 19.750027170959676 1.0759700032605153 1.2764916856863384 72-73 78.74015748031496 18.9487183667259 1.1297500855871276 1.1813740673720132 74-75 83.37363670450655 14.29510762358644 1.1384570239266172 1.1927986479803936 76 94.73323841714577 2.864874851919879 1.147688863288085 1.2541978676462597 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 0.5 15 1.5 16 3.0 17 3.0 18 3.5 19 6.5 20 11.0 21 13.0 22 16.5 23 23.0 24 24.5 25 23.0 26 30.0 27 48.5 28 63.5 29 67.0 30 62.5 31 66.0 32 86.5 33 99.0 34 117.0 35 125.5 36 140.5 37 165.0 38 156.5 39 158.5 40 188.0 41 241.5 42 276.0 43 313.0 44 839.0 45 4153.5 46 6979.0 47 6964.5 48 3803.0 49 546.5 50 437.0 51 337.5 52 217.5 53 176.0 54 155.0 55 169.5 56 164.0 57 161.5 58 179.0 59 184.0 60 166.0 61 156.5 62 170.0 63 152.0 64 128.5 65 125.5 66 125.5 67 123.0 68 114.0 69 95.5 70 94.5 71 103.0 72 104.5 73 90.5 74 83.5 75 92.0 76 94.5 77 96.5 78 98.5 79 101.0 80 110.0 81 114.5 82 117.5 83 125.0 84 131.5 85 142.5 86 152.5 87 158.0 88 193.5 89 225.0 90 245.5 91 299.5 92 329.0 93 360.0 94 430.5 95 570.5 96 671.0 97 851.0 98 1579.0 99 33111.5 100 64096.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0010867911404786228 3 0.0 4 0.0032603734214358684 5 0.0 6 0.004347164561914491 7 0.0010867911404786228 8 0.0 9 0.0 10-11 5.433955702393114E-4 12-13 0.0010867911404786228 14-15 5.433955702393114E-4 16-17 0.0 18-19 0.0021735822809572456 20-21 0.002716977851196557 22-23 0.0010867911404786228 24-25 0.0016301867107179342 26-27 0.0021735822809572456 28-29 0.0021735822809572456 30-31 0.0032603734214358684 32-33 0.0038037689916751794 34-35 0.002716977851196557 36-37 0.0016301867107179342 38-39 0.0021735822809572456 40-41 5.433955702393114E-4 42-43 0.0032603734214358684 44-45 0.0010867911404786228 46-47 0.0016301867107179342 48-49 0.0010867911404786228 50-51 0.0016301867107179342 52-53 0.0016301867107179342 54-55 0.0010867911404786228 56-57 0.0016301867107179342 58-59 0.002716977851196557 60-61 0.0016301867107179342 62-63 0.0016301867107179342 64-65 0.002716977851196557 66-67 0.002716977851196557 68-69 0.0016301867107179342 70-71 0.004347164561914491 72-73 0.002716977851196557 74-75 0.0038037689916751794 76 0.0032603734214358684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 92014.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.847262373117134 #Duplication Level Percentage of deduplicated Percentage of total 1 88.48812744214007 9.598539352707197 2 4.949403867347961 1.0737496467928793 3 2.184149884781084 0.7107614058730194 4 1.252379521090071 0.5433955702393114 5 0.9317703636910128 0.5053578803225596 6 0.35066626590521993 0.2282261395005108 7 0.2604949403867348 0.19779598756710934 8 0.12022843402464682 0.10433194948594779 9 0.18034265103697023 0.1760601647575369 >10 1.0620178338843802 2.2659595278979285 >50 0.1302474701933674 1.008542178364162 >100 0.040076144674882276 0.8466102984328473 >500 0.020038072337441138 1.1726476405764341 >1k 0.010019036168720569 1.1574325646097334 >5k 0.0 0.0 >10k+ 0.020038072337441138 80.41058969287282 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 63042 68.51348707805333 No Hit CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG 10947 11.897102614819485 No Hit TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT 1065 1.1574325646097334 No Hit CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG 552 0.5999087095441998 No Hit ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC 527 0.5727389310322342 No Hit TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG 336 0.3651618232008173 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 203 0.22061860151716042 No Hit CTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGG 130 0.14128284826222096 No Hit CTTATACACATCTGACGCTGCCGACGACGATGCTGGTGTAGATCTCGGTG 110 0.1195470254526485 No Hit CTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT 99 0.10759232290738366 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0032603734214358684 0.0 2 0.0 0.0 0.0 0.0032603734214358684 0.0 3 0.0 0.0 0.0 0.0032603734214358684 0.0 4 0.0 0.0 0.0 0.009781120264307606 0.0 5 0.0 0.0 0.0 0.009781120264307606 0.0 6 0.0 0.0 0.0 0.009781120264307606 0.0 7 0.0 0.0 0.0 0.009781120264307606 0.0 8 0.0 0.0 0.0 0.009781120264307606 0.0 9 0.0 0.0 0.0 0.009781120264307606 0.0 10 0.0 0.0 0.0 0.011954702545264852 0.0 11 0.0 0.0 0.0 0.019562240528615212 0.0 12 0.0 0.0 0.0 0.019562240528615212 0.0 13 0.0 0.0 0.0 0.023909405090529703 0.0 14 0.0 0.0 0.0 0.026082987371486947 0.0 15 0.0 0.0 0.0 0.03151694307388006 0.0 16 0.0 0.0 0.0 0.03151694307388006 0.0 17 0.0 0.0 0.0 0.03151694307388006 0.0 18 0.0 0.0 0.0 0.0380376899167518 0.0 19 0.0 0.0 0.0 0.04673201904058078 0.0 20 0.0 0.0 0.0 0.04999239246201665 0.0 21 0.0 0.0 0.0 0.05759993044536701 0.0 22 0.0 0.0 0.0 0.0619470950072815 0.0 23 0.0 0.0 0.0 0.06846784185015324 0.0 24 0.0 0.0 0.0 0.07064142413111048 0.0 25 0.0 0.0 0.0 0.07172821527158911 0.0 26 0.0 0.0 0.0 0.07172821527158911 0.0 27 0.0 0.0 0.0 0.08476970895733257 0.0 28 0.0 0.0 0.0 0.0858565000978112 0.0 29 0.0 0.0 0.0 0.09237724694068294 0.0 30 0.0 0.0 0.0 0.09889799378355468 0.0 31 0.0 0.0 0.0 0.10541874062642641 0.0 32 0.0 0.0 0.0 0.1141130697502554 0.0 33 0.0 0.0 0.0 0.12280739887408437 0.0 34 0.0 0.0 0.0 0.12715456343599887 0.0 35 0.0 0.0 0.0 0.15432434194796443 0.0 36 0.0 0.0 0.0 0.17388658247657965 0.0 37 0.0 0.0 0.0 0.21735822809572455 0.0 38 0.0 0.0 0.0 0.27930532310300604 0.0 39 0.0 0.0 0.0 0.31625622187927926 0.0 40 0.0 0.0 0.0 0.3792901080270394 0.0 41 0.0 0.0 0.0 0.42167496250570563 0.0 42 0.0 0.0 0.0 0.46949377268676507 0.0 43 0.0 0.0 0.0 0.5412219879583542 0.0 44 0.0 0.0 0.0 0.6270784880561654 0.0 45 0.0 0.0 0.0 0.6955463299063186 0.0 46 0.0 0.0 0.0 0.7444519312278566 0.0 47 0.0 0.0 0.0 0.8053122350946595 0.0 48 0.0 0.0 0.0 0.8531310452757189 0.0 49 0.0 0.0 0.0 0.9183385137044363 0.0 50 0.0 0.0 0.0 0.9705044884474102 0.0 51 0.0 0.0 0.0 1.0139761340665552 0.0 52 0.0 0.0 0.0 1.0444062859999566 0.0 53 0.0 0.0 0.0 1.0596213619666572 0.0 54 0.0 0.0 0.0 1.0965722607429305 0.0 55 0.0 0.0 0.0 1.1346099506596823 0.0 56 0.0 0.0 0.0 1.1965570456669636 0.0 57 0.0 0.0 0.0 1.2259004064598866 0.0 58 0.0 0.0 0.0 1.2682852609385529 0.0 59 0.0 0.0 0.0 1.2932814571695612 0.0 60 0.0 0.0 0.0 1.3204512356815268 0.0 61 0.0 0.0 0.0 1.3356663116482275 0.0 62 0.0 0.0 0.0 1.3650096724411502 0.0 63 0.0 0.0 0.0 1.397613406655509 0.0 64 0.0 0.0 0.0 1.4356510965722606 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGATG 15 0.0022183862 70.00001 29 CAGTAAA 15 0.0022183862 70.00001 61 ATGCGCG 15 0.0022183862 70.00001 4 TCAGTAA 15 0.0022183862 70.00001 60 TTGATGG 15 0.0022183862 70.00001 30 GCGTCTG 50 0.0 70.00001 8 GACGACC 15 0.0022183862 70.00001 23 CTTGTGC 30 1.0138319E-7 70.00001 1 GGGGTAT 15 0.0022183862 70.00001 54 CGTTGAT 15 0.0022183862 70.00001 28 AAAAATG 15 0.0022183862 70.00001 70 TTGTGCG 30 1.0138319E-7 70.00001 2 TTTATAC 50 0.0 70.00001 3 TGCGCGT 40 1.3278623E-10 70.0 5 GATGCTG 40 1.3278623E-10 70.0 29 ATGGTGT 20 7.889973E-5 70.0 33 TTGGGCG 20 7.889973E-5 70.0 2 CGCGGCC 20 7.889973E-5 70.0 16 TGGCGCT 40 1.3278623E-10 70.0 13 CGATGCT 35 3.6634447E-9 70.0 28 >>END_MODULE