FastQCFastQC Report
Wed 25 May 2016
SRR1780152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92014
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT2359225.63957658617167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1601317.40278653248419No Hit
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT1074311.675397222161845No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG42954.667767948355685No Hit
CATAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT31873.463603364705371No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT27222.9582454843828114No Hit
CAAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT10141.1020062164453235No Hit
CTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT8550.9292064251092225No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC8000.8694329123828982No Hit
CAAAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT6620.7194557349968483No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC5110.5553502727845763No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTT4560.495576760058252TruSeq Adapter, Index 6 (95% over 21bp)
TCTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG3940.4281957093485774No Hit
CTAAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT3660.39776555741517594No Hit
CTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC2580.2803921142434847No Hit
CACTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC2160.23474688634338253No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT1780.19344882300519486No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1750.190188449583759No Hit
CCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1570.1706262090551438No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC1490.1619318799313148No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1460.15867150650987893No Hit
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGT1450.1575847153694003No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACACCATCTCGTATGCCGT1440.1564979242289217No Hit
CTTTACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC1390.15106396852652856No Hit
TCATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1380.14997717738604996No Hit
TATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCT1220.13258851913839198No Hit
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTAAGCCGT1220.13258851913839198No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGT1160.12606777229552024No Hit
CATATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT1120.12172060773360574No Hit
CATAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTAAGCCGT1030.11193948746929816No Hit
CACTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC940.10215836720499055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCAT150.002218386270.0000153
ACTCGAA150.002218386270.0000138
AATCTCC150.002218386270.000019
AGCTTAA150.002218386270.0000155
TATACCG150.002218386270.0000143
TATATAC150.002218386270.000013
TACAGCA150.002218386270.0000152
AAATCGT150.002218386270.0000137
TAAGCTT500.070.0000153
AACTGCT150.002218386270.0000152
AATCAGC150.002218386270.0000151
TAAGCAG150.002218386270.0000143
GAAGTCT150.002218386270.0000146
GGATCTC150.002218386270.0000135
GTATTCA252.8202576E-670.0000149
ACCAACT150.002218386270.0000134
CTCTATA150.002218386270.000011
TCTACAG252.8202576E-670.0000150
GTATACC150.002218386270.0000142
AAAAGGC150.002218386270.0000167
Produced by FastQC (version 0.11.5)