FastQCFastQC Report
Wed 25 May 2016
SRR1780151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164870
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6522539.56147267544126No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG106446.455995632922909No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT9400.5701461757748529No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG6670.40456116940619885No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC5800.3517923212227816No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG3110.18863346879359494No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1880.11402923515497058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA150.00222151670.022
CGCGTCT207.9085185E-570.07
ATGCACA150.00222151670.04
CGATGCT252.385013E-455.99999628
CGTATCA14750.055.05084656
GCCGTAT14850.054.9158354
GTATCAT14800.054.86486457
TGGTCGC15200.054.57236549
CTCGGTG15100.054.470244
CCGTATC15000.054.3666755
CGGTGGT15150.054.29043246
GTGGTCG15350.054.26709748
TCTCGGT15100.054.2384143
CGCCGTA15000.054.13333553
TCGGTGG15350.054.03908545
GATCTCG15100.054.00662241
TCGCCGT15050.053.95348752
AAAAAGG7150.053.84615370
AGATCTC15150.053.82838440
GTAGATC15150.053.82838438
Produced by FastQC (version 0.11.5)