FastQCFastQC Report
Wed 25 May 2016
SRR1780151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164870
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT2636515.991387153514891No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155589.436525747558683No Hit
CATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT114826.964274883241342No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG46822.839813186146661No Hit
CATAAACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT34192.0737550797598105No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT29941.8159762236913932No Hit
CAAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT11030.6690119488081518No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC10900.6611269485048826No Hit
CTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT8790.5331473281979742No Hit
CAAAAACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT6910.4191180930430036No Hit
ATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCTT6430.3900042457693941Illumina PCR Primer Index 10 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC6050.3669557833444532No Hit
TCTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG4500.27294231819008913No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT3820.23169770121914238No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC2930.1777157760659914No Hit
CTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC2790.1692242372778553No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC2140.12979923576150906No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG2010.12191423545823983No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG1960.1188815430339055No Hit
TATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCT1950.11827500454903864Illumina PCR Primer Index 10 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG1720.10432461939710075No Hit
TCATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG1710.10371808091223388No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCCC252.8334853E-669.9863539
ATCGTAA150.002223234669.9863539
GCCGACA252.8334853E-669.9863546
GCCGTAT207.916159E-569.9863546
TGCCGTA207.916159E-569.9863545
CATATAC14100.065.270961
ATATACA14050.065.005122
AGGGGGG54450.064.9092970
AACACAT12200.062.8156175
TAAACAC9400.061.796463
AAACACA13100.060.9041564
TCTTTAT1100.060.442761
AACTCGT601.8189894E-1258.3219637
ATAAACA5750.057.206232
TGCCGAC800.056.8639145
CAAAAAC3000.055.9890821
ATATCTC509.749783E-1055.98908235
AATGACG252.3873123E-455.98908243
GTAAGAG252.3873123E-455.98908240
TCACGTA252.3873123E-455.98908238
Produced by FastQC (version 0.11.5)