FastQCFastQC Report
Wed 25 May 2016
SRR1780150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108125
Sequences flagged as poor quality0
Sequence length76
%GC93

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8452078.16878612716764No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG59945.543583815028902No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT5160.4772254335260116No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3960.36624277456647397No Hit
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG3100.28670520231213875No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC2880.26635838150289015Illumina Single End PCR Primer 1 (95% over 22bp)
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2160.1997687861271676No Hit
TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG1710.15815028901734104No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1180.10913294797687861No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1130.10450867052023122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAACT252.8236082E-670.000017
AAACAAC252.8236082E-670.000016
CGAATCA252.8236082E-670.0000156
CGAACAA252.8236082E-670.0000125
CTAATAA252.8236082E-670.000011
TAATAAA252.8236082E-670.000012
AATAAAC252.8236082E-670.000013
GTTGTGG500.070.000011
CGCTGCG150.00221944170.016
CGCTGAC207.896221E-570.016
CACACTC150.00221944170.014
ACGCTGG150.00221944170.015
ACGCTGA207.896221E-570.015
TAAACAA353.6707206E-970.05
CACATAT150.00221944170.07
CTTTACA150.00221944170.01
TGCGCGG207.896221E-570.05
ATAAGAG207.896221E-570.030
ACTGACG150.00221944170.011
ATGACGC150.00221944170.012
Produced by FastQC (version 0.11.5)