##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780150_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 108125 Sequences flagged as poor quality 0 Sequence length 76 %GC 93 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.1677225433526 32.0 27.0 32.0 13.0 32.0 2 29.016379190751444 32.0 32.0 32.0 13.0 32.0 3 28.937479768786126 32.0 32.0 32.0 13.0 32.0 4 28.55792832369942 32.0 32.0 32.0 13.0 32.0 5 29.28889710982659 32.0 32.0 32.0 13.0 32.0 6 33.371375722543355 37.0 37.0 37.0 13.0 37.0 7 32.898293641618494 37.0 37.0 37.0 13.0 37.0 8 33.462816184971096 37.0 37.0 37.0 13.0 37.0 9 33.52026820809249 37.0 37.0 37.0 22.0 37.0 10-11 33.45001156069364 37.0 37.0 37.0 17.5 37.0 12-13 33.116485549132946 37.0 37.0 37.0 13.0 37.0 14-15 33.89987514450867 37.0 37.0 37.0 20.0 37.0 16-17 34.100813872832376 37.0 37.0 37.0 27.0 37.0 18-19 33.54878150289017 37.0 37.0 37.0 17.5 37.0 20-21 34.11136184971098 37.0 37.0 37.0 24.5 37.0 22-23 34.23971329479769 37.0 37.0 37.0 27.0 37.0 24-25 33.80711213872832 37.0 37.0 37.0 22.0 37.0 26-27 34.2644161849711 37.0 37.0 37.0 27.0 37.0 28-29 33.9790612716763 37.0 37.0 37.0 22.0 37.0 30-31 33.89800693641619 37.0 37.0 37.0 22.0 37.0 32-33 33.767292485549135 37.0 37.0 37.0 22.0 37.0 34-35 33.88220578034682 37.0 37.0 37.0 22.0 37.0 36-37 34.71921387283237 37.0 37.0 37.0 29.5 37.0 38-39 34.92763005780347 37.0 37.0 37.0 29.5 37.0 40-41 34.475375722543355 37.0 37.0 37.0 27.0 37.0 42-43 34.264679768786124 37.0 37.0 37.0 27.0 37.0 44-45 34.13143583815029 37.0 37.0 37.0 24.5 37.0 46-47 34.42688554913295 37.0 37.0 37.0 27.0 37.0 48-49 34.95593988439306 37.0 37.0 37.0 32.0 37.0 50-51 35.26975722543352 37.0 37.0 37.0 32.0 37.0 52-53 34.4182289017341 37.0 37.0 37.0 29.5 37.0 54-55 34.662307514450866 37.0 37.0 37.0 29.5 37.0 56-57 34.54707514450867 37.0 37.0 37.0 29.5 37.0 58-59 34.19692485549133 37.0 37.0 37.0 24.5 37.0 60-61 33.961720231213874 37.0 37.0 37.0 24.5 37.0 62-63 33.63643930635838 37.0 37.0 37.0 17.5 37.0 64-65 33.45245780346821 37.0 37.0 37.0 17.5 37.0 66-67 33.47898265895954 37.0 37.0 37.0 17.5 37.0 68-69 33.66082312138728 37.0 37.0 37.0 22.0 37.0 70-71 33.780411560693636 37.0 37.0 37.0 22.0 37.0 72-73 34.01313294797688 37.0 37.0 37.0 24.5 37.0 74-75 33.769054335260115 37.0 37.0 37.0 17.5 37.0 76 34.91608786127168 37.0 37.0 37.0 32.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 1.2042214214542817 11101 2 -0.14329690097816083 11101 3 0.7252665213322835 11101 4 1.149432314732195 11101 5 -0.7994107270718587 11101 6 -0.04634539792762382 11101 7 -0.5663593681077472 11101 8 -0.6219622082009124 11101 9 -0.8857263217781437 11101 10-11 -0.16451659524864937 11101 12-13 0.10401954551766579 11101 14-15 0.5660677368453904 11101 16-17 0.5542881208539328 11101 18-19 -0.25154475808590604 11101 20-21 -0.41395030911356656 11101 22-23 0.8549918895492183 11101 24-25 -0.30954159958275085 11101 26-27 0.791014719399449 11101 28-29 0.025010234557306887 11101 30-31 0.23257940906010077 11101 32-33 0.26932097441405745 11101 34-35 1.4029353602754995 11101 36-37 0.20715091341863712 11101 38-39 -0.27469766646149196 11101 40-41 0.9506931685725704 11101 42-43 -0.9711767183828428 11101 44-45 -0.6752137578738839 11101 46-47 0.38762958043034956 11101 48-49 0.03295455880326159 11101 50-51 0.3806171244997216 11101 52-53 0.596081449348489 11101 54-55 -0.01865684442207538 11101 56-57 0.6254200068903657 11101 58-59 -0.1580425502444811 11101 60-61 0.12868184548334938 11101 62-63 -1.17215320774271 11101 64-65 0.714151046244595 11101 66-67 -0.18053156684135985 11101 68-69 -0.9248486394491238 11101 70-71 -0.5175194632921816 11101 72-73 0.00907586759921486 11101 74-75 -0.6809483383359165 11101 76 1.3440003631138993 11102 1 1.3825763998092633 11102 2 0.04977669209543123 11102 3 -0.7477493516835914 11102 4 0.1321162974161787 11102 5 -0.3445766722378032 11102 6 -0.0876152391974685 11102 7 0.41719041544203606 11102 8 -0.2268684131071197 11102 9 -1.1024651385169548 11102 10-11 -0.873607504339553 11102 12-13 -0.7580295165313942 11102 14-15 -0.7806855099078618 11102 16-17 -0.498237131671317 11102 18-19 -0.6874754940166454 11102 20-21 -0.3122187073819589 11102 22-23 -0.4116747771174545 11102 24-25 -0.9181996082407622 11102 26-27 -1.2213950930103579 11102 28-29 0.2541588637059391 11102 30-31 -0.18358220710151585 11102 32-33 -1.4132187081256262 11102 34-35 -0.4037024463623098 11102 36-37 0.19950300577072966 11102 38-39 -0.418853510617339 11102 40-41 -0.84151462363522 11102 42-43 0.520453873247753 11102 44-45 -0.6316351142952428 11102 46-47 -0.6446936518928794 11102 48-49 0.16816379401249293 11102 50-51 0.02318566706826175 11102 52-53 -0.11127785801081558 11102 54-55 -1.0631012888665197 11102 56-57 -0.4178700363996768 11102 58-59 -0.08300647520839988 11102 60-61 0.041380258181767715 11102 62-63 0.625249389659885 11102 64-65 -0.46290523081167834 11102 66-67 0.4289922426824475 11102 68-69 -0.2505340651345449 11102 70-71 -0.5303621761349007 11102 72-73 0.33692579544914025 11102 74-75 -0.2784952358828079 11102 76 1.6859917051052449 11103 1 0.9159097331425947 11103 2 -0.15498521266647458 11103 3 0.21098080704656752 11103 4 -0.686931321631441 11103 5 0.23955031188917886 11103 6 0.125083173500947 11103 7 0.5648094630610814 11103 8 -0.685598571837275 11103 9 -0.19611593216774992 11103 10-11 -0.43075036148240997 11103 12-13 -0.26675967526155375 11103 14-15 0.41931449009214106 11103 16-17 -0.2450625284967174 11103 18-19 -0.9969993035404556 11103 20-21 0.2711146259513697 11103 22-23 -0.3735795390222165 11103 24-25 0.593705153663997 11103 26-27 0.789716018100755 11103 28-29 -0.7061585966115302 11103 30-31 0.3338781103587962 11103 32-33 -0.06639331130023152 11103 34-35 0.4042340615741935 11103 36-37 -0.5084335021657793 11103 38-39 0.20971791795408734 11103 40-41 -0.3700860522066449 11103 42-43 -0.08351438072050144 11103 44-45 -0.771317653977782 11103 46-47 0.09736984017060735 11103 48-49 0.22451300036170352 11103 50-51 0.4676301115127046 11103 52-53 0.3649126181796589 11103 54-55 0.5313431555779289 11103 56-57 0.27736805883841953 11103 58-59 0.4614379692360373 11103 60-61 0.39296755976906894 11103 62-63 0.2966779610884629 11103 64-65 0.6680471501407013 11103 66-67 -0.480531566841357 11103 68-69 0.8550214904210094 11103 70-71 0.15455845878574337 11103 72-73 1.0908940494173933 11103 74-75 1.2722984149108427 11103 76 0.3764678955814347 11104 1 1.3203053375381977 11104 2 1.104721747040486 11104 3 0.7021895982553588 11104 4 0.4233250886249671 11104 5 0.6670228393617066 11104 6 -0.41301206459429096 11104 7 -0.038853540601920145 11104 8 0.6144014281627221 11104 9 0.7888657528139333 11104 10-11 0.587930957198914 11104 12-13 0.8314088229069441 11104 14-15 0.8401936109712622 11104 16-17 0.13424150080731323 11104 18-19 0.4773596708185224 11104 20-21 -0.1977498429130975 11104 22-23 1.1844790690363993 11104 24-25 1.1568919668508144 11104 26-27 0.3311079594926909 11104 28-29 0.7205813301284039 11104 30-31 0.5771015535822457 11104 32-33 -0.04478158968851176 11104 34-35 0.44489340223353935 11104 36-37 -0.3386532823855575 11104 38-39 -0.2705018622656894 11104 40-41 0.3691080869874952 11104 42-43 0.2930423958362738 11104 44-45 0.06860908594895676 11104 46-47 -0.039992797192020646 11104 48-49 -0.019259893411195605 11104 50-51 0.4456520895346898 11104 52-53 0.868575621842659 11104 54-55 0.3557021299369012 11104 56-57 0.4768186082889656 11104 58-59 -0.2402103824123074 11104 60-61 1.1350921018936049 11104 62-63 0.44393070834120607 11104 64-65 0.23489696699051876 11104 66-67 0.1386992023894109 11104 68-69 0.6081350435345598 11104 70-71 0.588074942302228 11104 72-73 -0.4539777454544023 11104 74-75 0.8906134332258588 11104 76 -0.3762793571658207 11105 1 -1.9594149421820788 11105 2 -0.17966053734179965 11105 3 0.20318859925436072 11105 4 -0.5518663865665054 11105 5 -1.4643457920069238 11105 6 -0.7917999433821734 11105 7 0.6855886838403009 11105 8 -1.1778063640450682 11105 9 0.10388406783224724 11105 10-11 0.0679509372188889 11105 12-13 0.24557798707610345 11105 14-15 -0.4754907047130601 11105 16-17 0.3387037052695163 11105 18-19 0.05494874840759678 11105 20-21 -0.3879762831395368 11105 22-23 -0.4917613572040267 11105 24-25 -0.8900610801022353 11105 26-27 -0.5128813844966515 11105 28-29 -0.08278197323490133 11105 30-31 -0.6258621493814616 11105 32-33 -0.5358738307807442 11105 34-35 0.0587795161196496 11105 36-37 -0.8538880476203232 11105 38-39 -0.7954768872407172 11105 40-41 -0.3285276106482087 11105 42-43 -1.012735159941279 11105 44-45 0.21179922913910332 11105 46-47 -0.23704574424497338 11105 48-49 -0.443668817820118 11105 50-51 0.42866907255167064 11105 52-53 -0.6610614077943708 11105 54-55 -1.4238516496168785 11105 56-57 0.07347195494230618 11105 58-59 -0.7099906021925264 11105 60-61 -0.644045427243924 11105 62-63 0.5213532857637801 11105 64-65 -0.11442038232682705 11105 66-67 0.12596193965214297 11105 68-69 -0.1365369511374297 11105 70-71 -0.11622076199348186 11105 72-73 0.051933010456352235 11105 74-75 -0.38094833833591935 11105 76 -1.032623013509479 11106 1 0.4778144950473582 11106 2 1.5140624063811465 11106 3 0.4347903308560923 11106 4 1.0964020117018904 11106 5 1.4014550737939437 11106 6 1.3488926973104682 11106 7 1.6909999392515545 11106 8 2.162020475781773 11106 9 0.9253126392608237 11106 10-11 1.1621067813747317 11106 12-13 1.1308593723574916 11106 14-15 0.8859811567588096 11106 16-17 0.8489850905508973 11106 18-19 1.3958578393166832 11106 20-21 1.2473051021418513 11106 22-23 0.5407061752635016 11106 24-25 1.468705153663997 11106 26-27 0.9909064942912309 11106 28-29 1.621222355769433 11106 30-31 0.4195923960730852 11106 32-33 0.43003526012834214 11106 34-35 0.35423406157419635 11106 36-37 1.120137926405647 11106 38-39 0.235908394144559 11106 40-41 0.2180091858885902 11106 42-43 0.9748189526128286 11106 44-45 0.7036823460222195 11106 46-47 0.24022698302776035 11106 48-49 0.4816558575045562 11106 50-51 0.3307253496079454 11106 52-53 1.1684840467510824 11106 54-55 0.3099145841493538 11106 56-57 0.349987106457462 11106 58-59 0.5876284454265175 11106 60-61 1.3905866073881086 11106 62-63 0.5716779610884544 11106 64-65 1.1204281025216574 11106 66-67 1.030182718872922 11106 68-69 1.168116728516246 11106 70-71 1.0688441730714544 11106 72-73 0.9777988113221525 11106 74-75 0.5211079387203625 11106 76 0.4359917051052449 11107 1 -0.09897960287350216 11107 2 1.241594264194692 11107 3 0.6041397607689021 11107 4 1.27363205865025 11107 5 0.2612806941829433 11107 6 0.7536546020723733 11107 7 0.1368416562200352 11107 8 0.06510565351483422 11107 9 0.7859766231642382 11107 10-11 0.7531530590407272 11107 12-13 0.6023550128672213 11107 14-15 0.7535197214804725 11107 16-17 0.34839823609080867 11107 18-19 0.010042949980672233 11107 20-21 -0.050313945477199695 11107 22-23 -0.44923346256345553 11107 24-25 0.39199489209457994 11107 26-27 0.3887099818834443 11107 28-29 1.149978224314026 11107 30-31 0.029250343759194664 11107 32-33 0.4946730870901135 11107 34-35 0.7179161541295258 11107 36-37 0.6161137815364341 11107 38-39 0.4201806946140465 11107 40-41 0.46733849507705827 11107 42-43 -0.16208580929193062 11107 44-45 -0.21759531997375348 11107 46-47 0.15421088644828274 11107 48-49 -0.5439980660366857 11107 50-51 0.4364429887762924 11107 52-53 -0.22965478624690405 11107 54-55 -0.27358642188686844 11107 56-57 -0.1274609150046473 11107 58-59 -0.9967109241241232 11107 60-61 -0.5685012531082023 11107 62-63 -1.2640866264366935 11107 64-65 -0.12823051585527878 11107 66-67 0.1546797007642695 11107 68-69 0.4760476473626625 11107 70-71 -0.4135501931257295 11107 72-73 -0.5640355280473912 11107 74-75 0.18819378714423607 11107 76 0.3030272517182553 11108 1 0.30162401885688084 11108 2 -0.36927092695218633 11108 3 0.08240937847513763 11108 4 0.08449724979713125 11108 5 -0.874735402396535 11108 6 -0.0034882550704864457 11108 7 -0.32090482265320475 11108 8 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48-49 0.18196641651076817 11109 50-51 -0.45680053030923773 11109 52-53 -0.11748903813504086 11109 54-55 0.02627069180981323 11109 56-57 -0.16838763474336815 11109 58-59 0.29518538124431615 11109 60-61 0.6141890918601618 11109 62-63 -0.3944193474001523 11109 64-65 -0.5778120631098176 11109 66-67 0.40932350562240316 11109 68-69 0.9261395028433697 11109 70-71 0.18840939046275906 11109 72-73 0.7937926001420337 11109 74-75 -0.4020287072423727 11109 76 -0.9972381085593511 11110 1 0.08411898867579382 11110 2 -0.25136348228418015 11110 3 -0.19867714063954978 11110 4 -0.27566371599763784 11110 5 -0.13308550300015654 11110 6 -0.8238101866600189 11110 7 0.1509261632622909 11110 8 0.47355635774018623 11110 9 -0.2281078838780104 11110 10-11 -1.1200863775789927 11110 12-13 -1.0525745645975704 11110 14-15 -1.1408464757026309 11110 16-17 -1.031883454050039 11110 18-19 -0.49699930354045563 11110 20-21 -0.944680142660296 11110 22-23 -0.6675433217183837 11110 24-25 -0.7981459529758368 11110 26-27 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-0.588434861456868 21612 48-49 0.12858171645573435 21612 50-51 -0.04412395720338225 21612 52-53 0.44176614440027606 21612 54-55 -0.011061907713212804 21612 56-57 -0.10472959034784424 21612 58-59 0.1733728697785395 21612 60-61 0.5210869087744854 21612 62-63 -0.030265981045353385 21612 64-65 -0.48159118620649366 21612 66-67 -0.22230371874010046 21612 68-69 0.22003053201233058 21612 70-71 0.7808694895271486 21612 72-73 0.14152696080979865 21612 74-75 -0.9269782577835528 21612 76 0.20015686484002515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 8.0 12 46.0 13 123.0 14 208.0 15 358.0 16 674.0 17 1012.0 18 1184.0 19 1180.0 20 1059.0 21 1040.0 22 1081.0 23 1068.0 24 1111.0 25 1226.0 26 1342.0 27 1434.0 28 1563.0 29 1717.0 30 2069.0 31 2424.0 32 3082.0 33 4383.0 34 6850.0 35 12154.0 36 59726.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 87.10566473988439 1.2892485549132948 1.868208092485549 9.736878612716763 2 85.37618497109827 0.8998843930635838 11.802080924855492 1.9218497109826589 3 85.04416184971099 1.2661271676300578 12.508670520231213 1.1810404624277455 4 87.10888726519363 8.488637729950703 3.109479194606043 1.2929958102496277 5 86.16046242774567 2.454566473988439 10.909595375722542 0.4753757225433526 6 88.26140840901944 9.128576977858344 1.5787721277815798 1.0312424853406337 7 86.92254335260115 2.3565317919075146 1.4927167630057803 9.228208092485549 8 88.20346820809249 9.179190751445086 1.0145664739884395 1.6027745664739883 9 86.63213872832371 1.5787283236994218 0.572485549132948 11.21664739884393 10-11 87.65641618497109 5.31514450867052 5.367861271676301 1.6605780346820809 12-13 86.98219653179191 1.5361849710982658 5.949595375722543 5.532023121387283 14-15 92.58450867052022 4.249710982658959 1.5815028901734103 1.584277456647399 16-17 91.83583815028902 1.5038150289017342 0.8994219653179191 5.7609248554913295 18-19 87.39595279493915 0.8536495135215124 5.147793274388665 6.602604417150679 20-21 92.0556966005836 0.9267352006770162 1.6046762207331569 5.412891978006225 22-23 91.9800785205943 0.7403502443941937 0.6719106215520072 6.607660613459484 24-25 88.76608692837358 4.941109009604758 0.6714544016795608 5.6213496603421085 26-27 92.46193045979403 4.935514152666602 0.7435872535826755 1.8589681339566888 28-29 88.57515445229551 5.595427472161592 1.2527283489327068 4.576689726610188 30-31 88.37799717912553 5.471571597031145 4.476866517144905 1.673564706698421 32-33 88.62534914263517 8.525554466251087 1.6883705443850465 1.1607258467286954 34-35 95.47648995597648 2.2025637231326995 1.4825570641115755 0.8383892567792534 36-37 93.27491329479768 0.9202312138728322 5.140346820809249 0.6645086705202312 38-39 92.9839489102739 0.8934227989290024 5.532562301442332 0.5900659893547656 40-41 92.63392031371389 4.763974695719729 1.779438422551885 0.822666568014502 42-43 89.35522804940646 4.920299472362622 4.804690932128538 0.9197815461023736 44-45 88.73618497109827 1.113063583815029 5.390982658959538 4.759768786127168 46-47 92.26948813162727 0.7010501879794864 1.7882329003408142 5.24122878005244 48-49 93.58005623173393 0.6598904960970737 4.676112611446118 1.0839406607228737 50-51 96.89017341040463 0.6252023121387282 1.615722543352601 0.8689017341040461 52-53 89.57678221301468 0.8120306314971699 4.804206281676594 4.80698087381155 54-55 92.70516857881424 0.6631244537546994 1.2790810593343784 5.352625908096685 56-57 92.5914450867052 0.8689017341040461 0.9771098265895953 5.562543352601156 58-59 89.48599967629309 4.5809151656685705 4.680801868251289 1.2522832897870473 60-61 88.48873289587466 1.6448630976328216 5.349851328792272 4.516552677700244 62-63 88.54237476242665 5.289735443863324 4.708461079588807 1.4594287141212212 64-65 88.298103557506 5.580655454179711 5.451636323278473 0.6696046650358155 66-67 88.20052994954844 10.098637206527721 1.0057943000365324 0.6950385438873141 68-69 88.22376057230322 10.509181545348186 0.6358410905946386 0.6312167917539503 70-71 88.42948836521032 10.324257334171877 0.6030113573304724 0.6432429432873368 72-73 89.30810920794643 9.471995116717842 0.5854389404757501 0.6344567348599756 74-75 92.59295227524971 6.217628560858306 0.598409174990751 0.5910099889012209 76 97.54908761318129 1.2661505877565367 0.5826697372436948 0.6020920618184845 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 3.5 16 3.5 17 1.0 18 3.5 19 4.5 20 5.0 21 7.0 22 10.5 23 14.5 24 17.5 25 20.0 26 22.0 27 29.0 28 30.5 29 27.0 30 35.0 31 44.5 32 64.5 33 83.0 34 83.0 35 83.0 36 102.0 37 121.0 38 127.0 39 144.0 40 191.0 41 415.0 42 603.0 43 1452.0 44 3744.5 45 2927.0 46 666.0 47 555.5 48 318.0 49 177.5 50 164.0 51 146.0 52 121.5 53 112.0 54 109.0 55 122.5 56 142.0 57 150.5 58 153.0 59 145.0 60 136.0 61 133.0 62 131.0 63 125.5 64 105.5 65 85.0 66 80.5 67 82.0 68 83.5 69 86.5 70 100.0 71 112.0 72 99.0 73 98.0 74 111.5 75 113.0 76 114.5 77 132.5 78 145.0 79 141.0 80 151.0 81 164.5 82 174.0 83 180.0 84 194.5 85 215.5 86 249.0 87 276.0 88 284.0 89 316.0 90 369.5 91 436.0 92 473.0 93 548.0 94 671.5 95 829.5 96 939.0 97 1203.5 98 2161.0 99 44200.0 100 85546.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.003699421965317919 5 0.0 6 0.0027745664739884392 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 9.248554913294797E-4 20-21 0.0032369942196531793 22-23 4.6242774566473987E-4 24-25 0.0013872832369942196 26-27 4.6242774566473987E-4 28-29 9.248554913294797E-4 30-31 0.0023121387283236996 32-33 0.0027745664739884392 34-35 9.248554913294797E-4 36-37 0.0 38-39 0.0013872832369942196 40-41 9.248554913294797E-4 42-43 0.0013872832369942196 44-45 0.0 46-47 0.0013872832369942196 48-49 9.248554913294797E-4 50-51 0.0 52-53 9.248554913294797E-4 54-55 4.6242774566473987E-4 56-57 0.0 58-59 0.0023121387283236996 60-61 4.6242774566473987E-4 62-63 4.6242774566473987E-4 64-65 0.0013872832369942196 66-67 0.0013872832369942196 68-69 4.6242774566473987E-4 70-71 9.248554913294797E-4 72-73 9.248554913294797E-4 74-75 0.004624277456647399 76 0.0018497109826589595 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 108125.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.178959537572254 #Duplication Level Percentage of deduplicated Percentage of total 1 91.11393785208068 9.274450867052023 2 3.8342722151553694 0.7805780346820809 3 1.6172996547337817 0.4938728323699422 4 0.8631655460657823 0.3514450867052023 5 0.6269307650372524 0.3190751445086705 6 0.26349264037797565 0.16092485549132948 7 0.1908050154461203 0.13595375722543354 8 0.17263310921315644 0.1405780346820809 9 0.07268762493185535 0.06658959537572254 >10 1.0630565146283846 1.9912138728323698 >50 0.08177357804833726 0.5077456647398844 >100 0.07268762493185535 1.5879768786127169 >500 0.009085953116481919 0.4772254335260116 >1k 0.0 0.0 >5k 0.009085953116481919 5.543583815028902 >10k+ 0.009085953116481919 78.16878612716764 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 84520 78.16878612716764 No Hit CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG 5994 5.543583815028902 No Hit TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT 516 0.4772254335260116 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 396 0.36624277456647397 No Hit CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG 310 0.28670520231213875 No Hit ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC 288 0.26635838150289015 Illumina Single End PCR Primer 1 (95% over 22bp) GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 216 0.1997687861271676 No Hit TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG 171 0.15815028901734104 No Hit GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 118 0.10913294797687861 No Hit TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 113 0.10450867052023122 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0027745664739884392 0.0 2 0.0 0.0 0.0 0.0027745664739884392 0.0 3 0.0 0.0 0.0 0.0027745664739884392 0.0 4 0.0 0.0 0.0 0.0055491329479768784 0.0 5 0.0 0.0 0.0 0.014797687861271676 0.0 6 0.0 0.0 0.0 0.014797687861271676 0.0 7 0.0 0.0 0.0 0.014797687861271676 0.0 8 0.0 0.0 0.0 0.014797687861271676 0.0 9 0.0 0.0 0.0 0.014797687861271676 0.0 10 0.0 0.0 0.0 0.014797687861271676 0.0 11 0.0 0.0 0.0 0.014797687861271676 0.0 12 0.0 0.0 0.0 0.014797687861271676 0.0 13 0.0 0.0 0.0 0.014797687861271676 0.0 14 0.0 0.0 0.0 0.017572254335260117 0.0 15 0.0 0.0 0.0 0.017572254335260117 0.0 16 0.0 0.0 0.0 0.017572254335260117 0.0 17 0.0 0.0 0.0 0.017572254335260117 0.0 18 0.0 0.0 0.0 0.022196531791907514 0.0 19 0.0 0.0 0.0 0.03052023121387283 0.0 20 0.0 0.0 0.0 0.03421965317919075 0.0 21 0.0 0.0 0.0 0.03976878612716763 0.0 22 0.0 0.0 0.0 0.04439306358381503 0.0 23 0.0 0.0 0.0 0.04901734104046243 0.0 24 0.0 0.0 0.0 0.05456647398843931 0.0 25 0.0 0.0 0.0 0.06566473988439306 0.0 26 0.0 0.0 0.0 0.06566473988439306 0.0 27 0.0 0.0 0.0 0.07028901734104047 0.0 28 0.0 0.0 0.0 0.0730635838150289 0.0 29 0.0 0.0 0.0 0.0776878612716763 0.0 30 0.0 0.0 0.0 0.07861271676300578 0.0 31 0.0 0.0 0.0 0.08138728323699422 0.0 32 0.0 0.0 0.0 0.08508670520231214 0.0 33 0.0 0.0 0.0 0.08878612716763006 0.0 34 0.0 0.0 0.0 0.09710982658959537 0.0 35 0.0 0.0 0.0 0.1008092485549133 0.0 36 0.0 0.0 0.0 0.11005780346820809 0.0 37 0.0 0.0 0.0 0.1424277456647399 0.0 38 0.0 0.0 0.0 0.16832369942196532 0.0 39 0.0 0.0 0.0 0.20254335260115608 0.0 40 0.0 0.0 0.0 0.2423121387283237 0.0 41 0.0 0.0 0.0 0.2589595375722543 0.0 42 0.0 0.0 0.0 0.2793063583815029 0.0 43 0.0 0.0 0.0 0.30982658959537573 0.0 44 0.0 0.0 0.0 0.33109826589595376 0.0 45 0.0 0.0 0.0 0.36254335260115605 0.0 46 0.0 0.0 0.0 0.3782658959537572 0.0 47 0.0 0.0 0.0 0.387514450867052 0.0 48 0.0 0.0 0.0 0.4032369942196532 0.0 49 0.0 0.0 0.0 0.4254335260115607 0.0 50 0.0 0.0 0.0 0.435606936416185 0.0 51 0.0 0.0 0.0 0.4578034682080925 0.0 52 0.0 0.0 0.0 0.4883236994219653 0.0 53 0.0 0.0 0.0 0.5169942196531792 0.0 54 0.0 0.0 0.0 0.5299421965317919 0.0 55 0.0 0.0 0.0 0.5567630057803469 0.0 56 0.0 0.0 0.0 0.5882080924855492 0.0 57 0.0 0.0 0.0 0.5965317919075145 0.0 58 0.0 0.0 0.0 0.6039306358381503 0.0 59 0.0 0.0 0.0 0.6242774566473989 0.0 60 0.0 0.0 0.0 0.6473988439306358 0.0 61 0.0 0.0 0.0 0.6658959537572254 0.0 62 0.0 0.0 0.0 0.6751445086705202 0.0 63 0.0 0.0 0.0 0.6908670520231214 0.0 64 0.0 0.0 0.0 0.7001156069364162 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAACT 25 2.8236082E-6 70.00001 7 AAACAAC 25 2.8236082E-6 70.00001 6 CGAATCA 25 2.8236082E-6 70.00001 56 CGAACAA 25 2.8236082E-6 70.00001 25 CTAATAA 25 2.8236082E-6 70.00001 1 TAATAAA 25 2.8236082E-6 70.00001 2 AATAAAC 25 2.8236082E-6 70.00001 3 GTTGTGG 50 0.0 70.00001 1 CGCTGCG 15 0.002219441 70.0 16 CGCTGAC 20 7.896221E-5 70.0 16 CACACTC 15 0.002219441 70.0 14 ACGCTGG 15 0.002219441 70.0 15 ACGCTGA 20 7.896221E-5 70.0 15 TAAACAA 35 3.6707206E-9 70.0 5 CACATAT 15 0.002219441 70.0 7 CTTTACA 15 0.002219441 70.0 1 TGCGCGG 20 7.896221E-5 70.0 5 ATAAGAG 20 7.896221E-5 70.0 30 ACTGACG 15 0.002219441 70.0 11 ATGACGC 15 0.002219441 70.0 12 >>END_MODULE