FastQCFastQC Report
Wed 25 May 2016
SRR1780150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108125
Sequences flagged as poor quality0
Sequence length76
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT3176129.374335260115608No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT2577223.835375722543354No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90948.41063583815029No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG42923.969479768786127No Hit
CAAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT16061.4853179190751444No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC13121.2134104046242775No Hit
CACTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC11471.0608092485549134No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT8300.7676300578034683No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT8240.7620809248554914No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT6740.6233526011560694No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC6220.5752601156069365No Hit
CTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT5610.5188439306358382No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT4260.39398843930635835RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT4020.3717919075144509No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT3750.3468208092485549No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT3060.28300578034682083No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT2780.25710982658959536No Hit
CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC2680.2478612716763006No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG2340.21641618497109824No Hit
CAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC2300.21271676300578032No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGA2090.19329479768786126No Hit
AAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT2050.18959537572254334RNA PCR Primer, Index 41 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1780.1646242774566474No Hit
CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT1690.15630057803468206No Hit
CATATACAAATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT1680.1553757225433526No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATTCCGT1640.1516763005780347No Hit
CAAATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT1600.14797687861271677No Hit
TCATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1570.14520231213872833No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGCCATCTCGTATGCCGT1490.1378034682080925No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC1410.13040462427745664No Hit
CATTACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1370.12670520231213872No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT1370.12670520231213872No Hit
CATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1340.1239306358381503No Hit
ACTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1310.12115606936416184No Hit
CAAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT1300.12023121387283237No Hit
CAAATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT1290.1193063583815029No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCAAGTATGCCGT1260.11653179190751445No Hit
CAAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT1260.11653179190751445No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGCCATCTCGTATGCCGT1260.11653179190751445No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT1200.11098265895953757No Hit
CATAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1190.11005780346820808No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCAGT1170.10820809248554912No Hit
CTTTACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1170.10820809248554912No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1140.1054335260115607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG455.456968E-1270.0000136
TAAACAC850.070.000013
CAACTCG455.456968E-1270.0000136
TACATCT252.8236082E-670.0000134
GTATACC252.8236082E-670.0000142
CAATACA455.456968E-1270.000011
TACCGTC252.8236082E-670.0000145
ATAGTAT252.8236082E-670.0000139
ATGCTAG252.8236082E-670.0000154
ATACCGT252.8236082E-670.0000144
TCTTTAT1000.070.000011
TTCAGAA252.8236082E-670.0000152
ATACATC252.8236082E-670.0000133
GCCGAAA252.8236082E-670.0000146
CATATAG252.8236082E-670.0000136
GAGACTA252.8236082E-670.0000124
CTTCATC150.00221944170.051
CTCGAAG207.896221E-570.028
TTATGAA150.00221944170.052
AGCCCAA353.6707206E-970.017
Produced by FastQC (version 0.11.5)