FastQCFastQC Report
Wed 25 May 2016
SRR1780149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165255
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7471445.21134005022541No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG55443.354815285467913No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT5260.3182959668391274No Hit
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG2910.17609149496233095No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC2690.16277873589301384Illumina Single End PCR Primer 1 (95% over 22bp)
TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG1850.11194820126471212No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGC207.9085745E-570.0000119
TCCGAAG150.002221525670.063
CCGGCAG150.002221525670.09
CGGCAGG150.002221525670.010
TTTCCCT150.002221525670.034
CTAAACG150.002221525670.031
CGTACGC150.002221525670.014
ACGTAAG150.002221525670.035
GCCGGCG453.8198777E-1062.22221820
CTGCCGG308.348514E-658.33333218
GCCGTAT8650.056.24277554
TCGCCGT8550.056.08186752
CTTATGC252.38503E-456.0000041
CGAATCA252.38503E-456.00000456
GTCGCCG8750.056.051
GTATCAT8850.054.97175257
GTAGATC9050.054.91712638
TGGTCGC8800.054.8863649
ATCTCGG9000.054.8333342
CGGTGGT9000.054.8333346
Produced by FastQC (version 0.11.5)