FastQCFastQC Report
Wed 25 May 2016
SRR1780149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165255
Sequences flagged as poor quality0
Sequence length76
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT3145819.03603521829899No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT2288313.847084808326526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82384.985023146047018No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG41452.508244833741793No Hit
CAAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT20531.2423224713321837No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC11960.7237299930410578No Hit
CACTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC10790.6529303198087804No Hit
CTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT6820.4126955311488306No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC6470.3915161417203715No Hit
ATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT6290.3806238843000212Illumina PCR Primer Index 5 (95% over 22bp)
AAACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT4020.2432604157211582Illumina PCR Primer Index 5 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT3790.2293425312395994No Hit
CATATACACATCTCCGAGCCCACGAGACAACCATTGATCTCGTATGCCGT2820.17064536625215576No Hit
CAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC2540.15370185470938852No Hit
CTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC2380.14401984811352153No Hit
CATAAACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT2310.13978397022782973No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT2190.13252246528092948No Hit
CATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGACGT2080.12586608574627092No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCATTGATCTCGTATGCCGT2050.12405070950954585No Hit
TCATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG2020.12223533327282078No Hit
CATATACAAATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT1850.11194820126471212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATC150.00222213569.9951643
GCGGCGA150.00222213569.9951611
CGTATCC207.911286E-569.99515541
TCGTATC207.911286E-569.99515540
AACTCGT252.831399E-669.99515537
CATATAC36550.068.558871
ATATACA36650.068.37182
AGGGGGG65050.066.928570
CCATAGA700.064.9955131
CATAGAT600.064.1622332
CGAATGC600.064.1622341
TCGAATG550.063.6319640
TGCCGAC550.063.6319645
GCCGACT453.8198777E-1062.21792246
CCGACTT453.8198777E-1062.21792247
CAAATAC4000.062.12071
TCACCGA401.0602889E-861.2457611
CACCGAG401.0602889E-861.2457612
ACGTATG352.9616967E-759.9958540
CACGTAT352.9616967E-759.9958539
Produced by FastQC (version 0.11.5)