FastQCFastQC Report
Wed 25 May 2016
SRR1780148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215829
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9032441.84979775655727No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG65563.0375899438907656No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT6170.2858744654332828No Hit
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG3940.18255192768349016No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3860.17884528955793708No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC2240.1037858675154868Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGC207.914056E-570.016
TGCACGT150.0022224570.05
GCCGTAT10550.056.0663554
GACGAAA252.3866723E-456.00000423
TCGCCGT10500.056.052
TGGTCGC10750.055.99999649
GTCGCCG10700.055.9345851
GATCTCG10750.055.67441641
CCGTATC10650.055.53990655
CGTATCA10600.055.471756
ATCTCGG10800.055.41666442
CGCCGTA10700.055.28037653
AGATCTC10850.054.8387140
CTCGGTG11050.054.7963844
GTAGATC11000.054.7272738
GGTCGCC10950.054.65753650
GTATCAT10700.054.6261757
TCTCGGT11100.054.54955343
CGGTGGT11250.054.44444346
TCGGTGG11350.054.27313245
Produced by FastQC (version 0.11.5)