FastQCFastQC Report
Wed 25 May 2016
SRR1780148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215829
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT3735117.30583007844173No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2965013.737727552831178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95264.413679348002353No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG52112.4144114090321507No Hit
CAAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT25571.1847342108799095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC19670.9113696491203684No Hit
CACTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC17180.7960005374625281No Hit
CTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT9100.42163008678166514No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC8320.3854903650575224No Hit
ATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT7010.3247941657515904TruSeq Adapter, Index 8 (95% over 21bp)
AAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT3940.18255192768349016RNA PCR Primer, Index 8 (95% over 21bp)
CAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC2970.13760894041115884No Hit
CATAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2940.13621895111407642No Hit
CTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC2780.1288056748629702No Hit
CATATACAAATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2720.12602569626880541No Hit
CATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACGT2640.1223190581432523No Hit
TCATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG2640.1223190581432523No Hit
ACTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG2400.11119914376659298No Hit
CCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG2270.10517585681256922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTC401.3460522E-1070.0069645
CGTCTGC150.002221573470.0069541
ATGACGA150.002221573470.0069544
GCCGTAT150.002221573470.0069546
CGTATTC150.002223616769.9907348
GGTCGTT150.002223616769.9907311
CCGTATT150.002223616769.9907347
GTCGTTA150.002223616769.9907312
ATCGCCT150.002223616769.9907332
GCTTTAC150.002223616769.990737
ATATACA43750.067.911012
CATATAC43700.067.748471
AGGGGGG82400.066.5660870
CAAATAC5550.065.5769041
CGAATGC700.065.00645441
GCCGTCA700.065.00645446
AAGCCGT453.8198777E-1062.228444
TCGAATG800.061.25608440
ATATCCG1150.060.86150410
CCGTCTT93450.058.56902347
Produced by FastQC (version 0.11.5)