FastQCFastQC Report
Wed 25 May 2016
SRR1780147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107490
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8071775.09256675039538No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG60765.652618848264955No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT4740.440971253139827No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC3380.31444785561447575Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG2890.2688622197413713No Hit
TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG2150.200018606381989No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1170.10884733463578007No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1150.10698669643687785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACG150.002219404670.04
GTCTGGC150.002219404670.010
AGGTGGT150.002219404670.046
CGGCGGA150.002219404670.022
TGGACGC150.002219404670.049
GGTGGAC150.002219404670.047
TGTGCAC150.002219404670.03
CGTCTGG150.002219404670.09
GGACGCC150.002219404670.050
AACATCT150.002219404670.07
AAAAAGG7550.059.80132370
CCGTATC8850.057.74011655
GCCGTAT8850.057.74011654
CGTATCA8950.057.48603456
TCGCCGT8850.057.34463552
CGCCGTA8850.057.34463553
TAAGGGT8900.057.02247231
GTCGCCG9050.056.85082651
GTATCAT8750.056.857
AAGGGTG9000.056.77777532
Produced by FastQC (version 0.11.5)