FastQCFastQC Report
Wed 25 May 2016
SRR1780147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107490
Sequences flagged as poor quality0
Sequence length76
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT3016428.062145315843335No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2355021.90901479207368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95058.842683040282818No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG41243.836635966136385No Hit
CAAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT24982.323937110428877No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC11361.056842496976463No Hit
CACTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC11061.0289329239929295No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC8370.7786770862405805No Hit
CTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT8090.7526281514559494No Hit
CATATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT7150.6651781561075449No Hit
ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT7150.6651781561075449TruSeq Adapter, Index 18 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT6180.574937203460787No Hit
AAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT4950.4605079542283003RNA PCR Primer, Index 18 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC3420.3181691320122802No Hit
CAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC3360.31258721741557355No Hit
CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT2720.2530467950507024No Hit
TCATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG2670.24839519955344685No Hit
CATAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2170.20187924458089124No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT1970.183272862591869No Hit
ACTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1950.1814122243929668No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1860.17303935249790678No Hit
CATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1720.16001488510559123No Hit
CATTACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC1720.16001488510559123No Hit
CATATACAAATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1640.15257233230998232No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC1570.14606009861382455No Hit
TATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCT1550.14419946041492232No Hit
CATAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1550.14419946041492232No Hit
CATATACACATCTCCGAGCCAACGAGACCCGGGCGCATCTCGTATGCCGT1470.13675690761931342No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC1450.1348962694204112No Hit
CATATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT1420.13210531212205787RNA PCR Primer, Index 24 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT1350.1255930784259001No Hit
CATATACACATCTCCGAGCCCACGAGACCAGGGCGCATCTCGTATGCCGT1340.12466275932644898No Hit
TAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC1280.11908084472974231No Hit
CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCAGT1270.11815052563029119No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1270.11815052563029119No Hit
CATATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT1190.1107079728346823No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGGCGCATCTCGTATGCCGT1190.1107079728346823No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1170.10884733463578007No Hit
CATATACACATCTCCGAGACCACGAGACCCGGGCGCATCTCGTATGCCGT1130.10512605823797562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA150.002215717570.02933531
ACCCGTG353.6598067E-970.02933527
ACCCGGT150.002215717570.02933527
CCCGTGC301.01252226E-770.02933528
CAGGGAG150.002215717570.02933529
AGCGCAT150.002215717570.02933532
GTGCGCA500.070.02933531
GGGCGAT150.002215717570.02933531
GACCCGC150.002215717570.02933526
AACGGGC150.002215717570.02933528
GACCAGT150.002215717570.02933526
TGCGCAT401.3278623E-1070.0293332
GCGCGCA207.8796234E-570.0293331
CCGAGCG207.8796234E-570.0293329
CGCGCAT207.8796234E-570.0293332
CTCGAAA150.002219813969.9967439
ATCCCAC150.002219813969.9967417
ACTGCAT150.002219813969.9967453
AATCCGA150.002219813969.9967411
TCCCACG150.002219813969.9967418
Produced by FastQC (version 0.11.5)