##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780147_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 107490 Sequences flagged as poor quality 0 Sequence length 76 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.82194622755605 32.0 32.0 32.0 27.0 32.0 2 17.709535770769374 13.0 8.0 27.0 8.0 32.0 3 28.470862405805192 32.0 27.0 32.0 13.0 32.0 4 31.001283840357242 32.0 32.0 32.0 27.0 32.0 5 30.569057586752255 32.0 32.0 32.0 27.0 32.0 6 34.188250069773936 37.0 32.0 37.0 27.0 37.0 7 34.19416689924644 37.0 37.0 37.0 27.0 37.0 8 31.56925295376314 37.0 27.0 37.0 13.0 37.0 9 33.4967810959159 37.0 32.0 37.0 22.0 37.0 10-11 34.85027909572983 37.0 37.0 37.0 29.5 37.0 12-13 35.04683691506187 37.0 37.0 37.0 29.5 37.0 14-15 34.820411201041956 37.0 37.0 37.0 27.0 37.0 16-17 31.867973765001395 37.0 32.0 37.0 17.5 37.0 18-19 33.630779607405344 37.0 34.5 37.0 24.5 37.0 20-21 34.519378546841565 37.0 37.0 37.0 27.0 37.0 22-23 33.843766862033675 37.0 34.5 37.0 24.5 37.0 24-25 33.958261233603125 37.0 34.5 37.0 24.5 37.0 26-27 33.54798120755419 37.0 34.5 37.0 22.5 37.0 28-29 32.31132198344032 37.0 32.0 37.0 17.5 37.0 30-31 33.599972090427016 37.0 37.0 37.0 22.0 37.0 32-33 33.77369987905851 37.0 37.0 37.0 24.5 37.0 34-35 34.045869383198436 37.0 37.0 37.0 27.0 37.0 36-37 32.35970787980278 37.0 32.0 37.0 20.0 37.0 38-39 31.843892455112105 37.0 29.5 37.0 13.0 37.0 40-41 33.12811424318541 37.0 34.5 37.0 17.5 37.0 42-43 33.82002977021118 37.0 37.0 37.0 24.5 37.0 44-45 34.246060098613825 37.0 37.0 37.0 27.0 37.0 46-47 33.577416503860825 37.0 34.5 37.0 22.0 37.0 48-49 32.28631035445157 37.0 32.0 37.0 17.5 37.0 50-51 33.22913759419481 37.0 34.5 37.0 22.0 37.0 52-53 32.192013210531215 37.0 29.5 37.0 17.5 37.0 54-55 32.37188110521909 37.0 32.0 37.0 17.5 37.0 56-57 32.39022234626477 37.0 32.0 37.0 17.5 37.0 58-59 32.34086426644339 37.0 29.5 37.0 17.5 37.0 60-61 33.15543771513629 37.0 34.5 37.0 17.5 37.0 62-63 34.32424876732719 37.0 37.0 37.0 27.0 37.0 64-65 34.48090520048376 37.0 37.0 37.0 27.0 37.0 66-67 34.15806586659224 37.0 37.0 37.0 27.0 37.0 68-69 33.16062889571123 37.0 32.0 37.0 22.0 37.0 70-71 29.310163736161503 34.5 20.0 37.0 13.0 37.0 72-73 32.56849939529259 37.0 34.5 37.0 13.0 37.0 74-75 34.04687877942134 37.0 37.0 37.0 27.0 37.0 76 34.57859335752163 37.0 37.0 37.0 32.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.38041838233755954 11101 2 1.879062590762853 11101 3 -1.100801966752595 11101 4 -1.0853494377371078 11101 5 -0.08784263964665939 11101 6 -0.6521254315580762 11101 7 -1.9496072138763907 11101 8 -3.2939358150162477 11101 9 -1.9224277934842107 11101 10-11 -1.2107458353792069 11101 12-13 -0.7748855648153281 11101 14-15 -0.30739921880420695 11101 16-17 -0.5013985398601619 11101 18-19 -1.907218653105673 11101 20-21 -0.24908380962763488 11101 22-23 -0.3174264166521752 11101 24-25 -2.6319078232174036 11101 26-27 -1.1961024430242304 11101 28-29 -1.7333938972455911 11101 30-31 -0.2684835624384334 11101 32-33 -2.116545638915138 11101 34-35 -1.4909514782963242 11101 36-37 -0.8029276395818314 11101 38-39 -0.6898313110170413 11101 40-41 -2.0081941820737867 11101 42-43 -2.1274501023179546 11101 44-45 -2.2305373479175614 11101 46-47 -3.1879991730294712 11101 48-49 -2.386887053164209 11101 50-51 -1.4460954000859125 11101 52-53 -2.5646830089998645 11101 54-55 -0.7973542099461532 11101 56-57 -2.3885054263852155 11101 58-59 -0.9892525365898095 11101 60-61 0.17258277899848906 11101 62-63 -0.48250487583372603 11101 64-65 -0.35682082096203516 11101 66-67 -1.0701243747351654 11101 68-69 0.13614621291571893 11101 70-71 -0.9690138444343006 11101 72-73 -2.3577079287626006 11101 74-75 -2.208107370971561 11101 76 -1.016815916600308 11102 1 -0.2905250138888569 11102 2 1.8998173077439837 11102 3 -0.5860849856205164 11102 4 -0.14761358868050678 11102 5 0.14272339808918844 11102 6 0.4018368325928634 11102 7 0.7062418427273869 11102 8 1.1275736189460162 11102 9 -1.7684655293332696 11102 10-11 -1.5928213070773225 11102 12-13 -1.050734621419096 11102 14-15 -0.07400299238911856 11102 16-17 -0.8321532568412948 11102 18-19 -1.8775960115962356 11102 20-21 -0.4998385266087624 11102 22-23 -0.5913886808031208 11102 24-25 -1.7226625401985416 11102 26-27 -2.280253386420455 11102 28-29 -1.5577335198871012 11102 30-31 -2.3820684680988116 11102 32-33 -0.9808852615566437 11102 34-35 0.36187871038291775 11102 36-37 0.9116006623049628 11102 38-39 -0.9836048959226993 11102 40-41 -0.6470621066020925 11102 42-43 -0.7072614230726657 11102 44-45 -0.5750656498043583 11102 46-47 -0.24988596548229935 11102 48-49 -0.09933988335289001 11102 50-51 -1.3864727585764811 11102 52-53 -2.5018528203206216 11102 54-55 0.013211827789696429 11102 56-57 -3.3758639169512534 11102 58-59 0.789992746429057 11102 60-61 -1.4421342021335875 11102 62-63 0.9456083317134443 11102 64-65 -1.0822925190752457 11102 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11103 46-47 -0.1898859654823042 11103 48-49 0.14066011664711198 11103 50-51 -0.5064727585764786 11103 52-53 -0.7818528203206228 11103 54-55 -1.2367881722103036 11103 56-57 0.4041360830487477 11103 58-59 -1.0700072535709424 11103 60-61 0.92786579786641 11103 62-63 -0.12439166828656312 11103 64-65 -0.29229251907523945 11103 66-67 -0.3055960728483669 11103 68-69 -0.5097028436880464 11103 70-71 -2.3088251651890204 11103 72-73 -0.408651324989016 11103 74-75 -0.4482960502168396 11103 76 -0.5594574260342711 11104 1 0.818760700396858 11104 2 2.592674450601127 11104 3 0.028915014379482074 11104 4 0.41667212560520994 11104 5 -0.6865623161965253 11104 6 1.1925511183071507 11104 7 -0.6423295858440454 11104 8 2.115430761803161 11104 9 1.4043916135238774 11104 10-11 -0.0392498785058919 11104 12-13 0.49819395000947253 11104 14-15 0.1895684361823129 11104 16-17 0.8653467431587067 11104 18-19 0.700261131260902 11104 20-21 -0.31090995518019326 11104 22-23 0.6771827477683132 11104 24-25 -0.10444825448424666 11104 26-27 1.343318042150976 11104 28-29 0.6633379086843263 11104 30-31 -0.1581398966702423 11104 32-33 1.9319718813004911 11104 34-35 -0.43990700390279613 11104 36-37 1.6573149480192484 11104 38-39 -0.27574775306556276 11104 40-41 0.9997236076836202 11104 42-43 0.8545242912130462 11104 44-45 0.01457720733850465 11104 46-47 0.599399748803414 11104 48-49 0.284945830932827 11104 50-51 -0.73147275857648 11104 52-53 -0.6654242488920481 11104 54-55 -0.23535960078173446 11104 56-57 -0.3551496312369693 11104 58-59 0.06499274642905561 11104 60-61 -0.3232056307050186 11104 62-63 -0.1683202397151291 11104 64-65 -0.7358639476466706 11104 66-67 -0.3955960728483703 11104 68-69 0.17708287059766548 11104 70-71 -2.290610879474734 11104 72-73 -0.33007989641758684 11104 74-75 0.21170394978315699 11104 76 1.4855425739657306 11105 1 -0.22507046843431056 11105 2 0.12709003501671035 11105 3 -1.3642668038023373 11105 4 0.24329550222858387 11105 5 -0.7427311473653546 11105 6 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0.20407111728840022 11105 66-67 0.5180402907879937 11105 68-69 0.7002971563119473 11105 70-71 -0.19337061973447334 11105 72-73 0.07316685682916102 11105 74-75 -0.701932413853207 11105 76 0.8696334830566386 11106 1 -1.2166788600427019 11106 2 1.507509615436291 11106 3 -0.6230080625435939 11106 4 0.19854025747333992 11106 5 -0.25881506344927274 11106 6 0.2126060633620952 11106 7 0.07854953503508 11106 8 -0.05242638105398356 11106 9 0.4499960091282702 11106 10-11 0.1571786929226775 11106 12-13 -0.15688846757294783 11106 14-15 0.2513816229954955 11106 16-17 0.20092366623563507 11106 18-19 -0.044519088519308525 11106 20-21 -1.0313769881472297 11106 22-23 -0.11292714234157586 11106 24-25 0.5319528444168427 11106 26-27 -0.467945694112764 11106 28-29 0.34149724934366077 11106 30-31 0.6733161472858029 11106 32-33 1.1506531999818108 11106 34-35 -0.038121289617080834 11106 36-37 0.43698527768957973 11106 38-39 -0.4628356651534702 11106 40-41 -0.8886005681405464 11106 42-43 -0.6972614230726748 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0.4827233980891883 21612 6 1.5151701659262002 21612 7 -0.2804248239392848 21612 8 1.520906952279347 21612 9 -0.28846552933326564 21612 10-11 1.297178692922678 21612 12-13 0.7359320452475728 21612 14-15 0.6959970076108846 21612 16-17 1.8511800764920388 21612 18-19 0.3124039884037657 21612 20-21 0.21682814005790618 21612 22-23 0.8019446525302101 21612 24-25 0.03400412646812612 21612 26-27 0.4730799469128826 21612 28-29 -0.421066853220438 21612 30-31 0.7712648652345209 21612 32-33 0.9757814051100198 21612 34-35 0.7152120437162495 21612 36-37 0.8682673289716334 21612 38-39 1.1063951040773041 21612 40-41 2.23293789339791 21612 42-43 -0.12392808973933711 21612 44-45 0.024934350195643162 21612 46-47 0.6434473678510315 21612 48-49 0.007326783313779117 21612 50-51 1.2601939080901872 21612 52-53 0.8814805130127112 21612 54-55 0.8998784944563596 21612 56-57 -0.4125305836179223 21612 58-59 1.049992746429055 21612 60-61 -0.12213420213358717 21612 62-63 0.17560833171344115 21612 64-65 0.4343741475914271 21612 66-67 -0.05226273951503657 21612 68-69 0.6869638229786119 21612 70-71 1.5145081681443173 21612 72-73 -0.7819846583223509 21612 74-75 0.07503728311648672 21612 76 0.3338759072990669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 2.0 18 9.0 19 25.0 20 113.0 21 298.0 22 596.0 23 853.0 24 1260.0 25 1588.0 26 2066.0 27 2521.0 28 3043.0 29 4027.0 30 5280.0 31 6994.0 32 10108.0 33 16129.0 34 23811.0 35 24411.0 36 4354.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.289858308446602 4.640561184143198 8.094932410431028 74.97464809697917 2 10.743424908595298 47.086678636883775 31.778135437114496 10.391761017406433 3 10.754588841648912 13.617207342146639 70.29463479983998 5.333569016364465 4 10.260489347846312 70.19164573448693 15.309331100567494 4.238533817099265 5 10.061401060563774 15.483300772164851 72.7630477253698 1.6922504419015723 6 11.325301204819278 75.42633855886868 8.848676559519934 4.399683676792111 7 11.248488231463392 13.877570006512233 6.267559773002141 68.60638198902224 8 10.815889850218625 75.29444599497627 3.702670015815425 10.186994138989673 9 10.961019629732998 11.975997767234162 1.9257605358638012 75.13722206716903 10-11 10.994148238424396 42.14663826065923 37.3945240908372 9.46468941007917 12-13 11.902502558377522 5.143734300865196 42.19648339380407 40.75727974695321 14-15 12.651409433435667 3.9087356963438458 7.627221136849939 75.81263373337055 16-17 49.242255093497064 36.854125965206066 2.0132105312122057 11.890408410084659 18-19 50.679833844235944 9.351145925881132 1.9136752892142954 38.055344940668625 20-21 14.092938878035167 4.3036561540608425 1.8369150618662202 79.76648990603778 22-23 12.738572033287282 40.95833507770599 1.8769449755090077 44.42614791349772 24-25 49.28502316580764 40.054983904880636 1.809072809482165 8.85092011982956 26-27 50.76039170989277 42.35816798864879 1.8910934846828407 4.990346816775604 28-29 15.003814805165804 10.150173061892888 2.085581897353828 72.76043023558748 30-31 47.21581288029624 3.1693678941590218 2.016616735825534 47.59820248971921 32-33 89.05434448879336 2.2845898344823734 2.196667317944567 6.464398358779691 34-35 53.607878162010294 3.815810284034348 2.8003386456036545 39.77597290835171 36-37 16.787142990045588 37.185784724160385 3.7175551214066425 42.30951716438739 38-39 11.837845380965671 8.85803330542376 39.7869569262257 39.517164387384874 40-41 11.811660945044517 3.764641305460195 42.40187185427075 42.02182589522454 42-43 48.87428247136863 3.564617116488505 39.634095285941555 7.927005126201308 44-45 14.016652711880177 40.091171271746205 42.141594566936455 3.750581449437157 46-47 49.04242897879249 5.280712282121774 8.939345307041414 36.737513432044324 48-49 47.40394455298168 2.9360870778677084 4.079449251093125 45.580519118057495 50-51 16.311284770676345 3.333798492883059 40.90845660061401 39.44646013582659 52-53 11.446646199646478 4.332030886594102 75.51679225974509 8.704530654014327 54-55 11.483195720432608 4.424700546575183 44.36701942086289 39.72508431212932 56-57 48.34310168387757 3.066331751790864 9.876732719322728 38.713833845008836 58-59 13.814228697423875 6.534278563919357 71.83240763999368 7.819085098663094 60-61 47.77560703321239 39.73346357800725 10.199088287282537 2.2918411014978135 62-63 12.671005074914293 82.93600770307798 2.0643876843784743 2.3285995376292568 64-65 10.480066986091083 85.3081825370982 1.8984044285249104 2.3133460482858075 66-67 10.589455665230862 85.23616370047168 1.7908809273507056 2.3834997069467576 68-69 12.390920085589357 83.49427853753838 1.7341148013768724 2.380686575495395 70-71 44.85673085868453 50.617731882035535 1.78249139454833 2.7430458647316027 72-73 91.37015932085126 4.331201302477032 1.7276427491568787 2.5709966275148273 74-75 93.9270595897102 1.8797971810020002 1.5820812206354375 2.6110620086523704 76 94.14145361170034 1.680250083733393 1.5593018495776114 2.6189944549886492 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 5.0 16 6.5 17 5.0 18 4.5 19 5.5 20 13.5 21 20.0 22 18.5 23 16.5 24 20.0 25 24.0 26 33.5 27 43.5 28 55.0 29 66.0 30 69.0 31 74.0 32 103.0 33 130.0 34 142.5 35 174.0 36 217.0 37 241.0 38 279.5 39 350.5 40 442.5 41 514.0 42 526.0 43 595.0 44 726.5 45 884.5 46 980.0 47 1138.0 48 1482.0 49 2125.5 50 2583.0 51 4283.0 52 11695.0 53 37128.0 54 56849.0 55 29909.5 56 2377.0 57 1097.5 58 411.0 59 335.0 60 221.0 61 160.5 62 138.0 63 134.0 64 123.5 65 122.0 66 108.5 67 90.0 68 90.5 69 90.5 70 73.5 71 57.0 72 56.5 73 47.5 74 40.0 75 41.0 76 34.0 77 22.5 78 18.5 79 19.0 80 18.0 81 10.5 82 6.5 83 9.0 84 8.0 85 11.0 86 13.5 87 12.0 88 10.0 89 8.5 90 7.0 91 8.5 92 12.0 93 6.5 94 3.0 95 10.0 96 15.0 97 23.0 98 99.0 99 4811.0 100 9455.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0027909572983533353 2 9.303190994511118E-4 3 9.303190994511118E-4 4 0.0 5 0.0 6 0.0046515954972555585 7 0.0 8 0.0 9 0.0 10-11 9.303190994511118E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.651595497255559E-4 20-21 0.0 22-23 0.0013954786491766676 24-25 0.0037212763978044473 26-27 0.011628988743138896 28-29 0.013024467392315565 30-31 0.007442552795608895 32-33 0.008372871895060006 34-35 0.0027909572983533353 36-37 0.0 38-39 0.0 40-41 0.0027909572983533353 42-43 0.0027909572983533353 44-45 0.0 46-47 0.004186435947530003 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0023257977486277792 56-57 0.0 58-59 0.0027909572983533353 60-61 0.0 62-63 4.651595497255559E-4 64-65 0.0046515954972555585 66-67 9.303190994511118E-4 68-69 0.0 70-71 0.0 72-73 0.0023257977486277792 74-75 0.0046515954972555585 76 0.0055819145967066705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 107490.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.823611498744071 #Duplication Level Percentage of deduplicated Percentage of total 1 79.92462480651457 11.048469625081403 2 9.12578235412881 2.523025397711415 3 4.186015209637257 1.7359754395757745 4 2.2747156605424323 1.257791422457903 5 1.0364089104246585 0.716345706577356 6 0.699912510936133 0.5805191180574937 7 0.48455481526347666 0.46888082612336035 8 0.2220876236624268 0.2456042422550935 9 0.1413284877851807 0.17583030979626013 >10 1.4267447338313481 3.964089682761187 >50 0.20189783969311528 1.9127360684714858 >100 0.19516791170334477 4.873941762024375 >500 0.03364963994885255 3.4365987533724067 >1k 0.026919711959082035 8.246348497534655 >5k 0.006729927989770509 8.842683040282818 >10k+ 0.013459855979541017 49.971160107917015 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 30164 28.062145315843335 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 23550 21.90901479207368 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 9505 8.842683040282818 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 4124 3.836635966136385 No Hit CAAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 2498 2.323937110428877 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC 1136 1.056842496976463 No Hit CACTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC 1106 1.0289329239929295 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC 837 0.7786770862405805 No Hit CTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 809 0.7526281514559494 No Hit CATATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT 715 0.6651781561075449 No Hit ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT 715 0.6651781561075449 TruSeq Adapter, Index 18 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT 618 0.574937203460787 No Hit AAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT 495 0.4605079542283003 RNA PCR Primer, Index 18 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC 342 0.3181691320122802 No Hit CAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC 336 0.31258721741557355 No Hit CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT 272 0.2530467950507024 No Hit TCATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 267 0.24839519955344685 No Hit CATAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 217 0.20187924458089124 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT 197 0.183272862591869 No Hit ACTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 195 0.1814122243929668 No Hit CCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 186 0.17303935249790678 No Hit CATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 172 0.16001488510559123 No Hit CATTACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC 172 0.16001488510559123 No Hit CATATACAAATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT 164 0.15257233230998232 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC 157 0.14606009861382455 No Hit TATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCT 155 0.14419946041492232 No Hit CATAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 155 0.14419946041492232 No Hit CATATACACATCTCCGAGCCAACGAGACCCGGGCGCATCTCGTATGCCGT 147 0.13675690761931342 No Hit CTTTACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC 145 0.1348962694204112 No Hit CATATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT 142 0.13210531212205787 RNA PCR Primer, Index 24 (95% over 21bp) CAAATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT 135 0.1255930784259001 No Hit CATATACACATCTCCGAGCCCACGAGACCAGGGCGCATCTCGTATGCCGT 134 0.12466275932644898 No Hit TAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC 128 0.11908084472974231 No Hit CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCAGT 127 0.11815052563029119 No Hit CTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 127 0.11815052563029119 No Hit CATATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT 119 0.1107079728346823 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGGGCGCATCTCGTATGCCGT 119 0.1107079728346823 No Hit CTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG 117 0.10884733463578007 No Hit CATATACACATCTCCGAGACCACGAGACCCGGGCGCATCTCGTATGCCGT 113 0.10512605823797562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0046515954972555585 0.0 6 0.0 0.0 0.0 0.0046515954972555585 0.0 7 0.0 0.0 0.0 0.0046515954972555585 0.0 8 0.0 0.0 0.0 0.0046515954972555585 0.0 9 0.0 0.0 0.0 0.0046515954972555585 0.0 10 0.0 0.0 0.0 0.0046515954972555585 0.0 11 0.0 0.0 0.0 0.0046515954972555585 0.0 12 0.0 0.0 0.0 0.0046515954972555585 0.0 13 0.0 0.0 0.0 0.0046515954972555585 0.0 14 0.0 0.0 0.0 0.0046515954972555585 0.0 15 0.0 0.0 0.0 0.012094148292864452 0.0 16 0.0 0.0 0.0 0.012094148292864452 0.0 17 0.0 0.0 0.0 0.012094148292864452 0.0 18 0.0 0.0 0.0 0.023257977486277795 0.0 19 0.0 0.0 0.0 0.03256116848078891 0.0 20 0.0 0.0 0.0 0.03535212577914225 0.0 21 0.0 0.0 0.0 0.04279467857475114 0.0 22 0.0 0.0 0.0 0.04837659317145781 0.0 23 0.0 0.0 0.0 0.04837659317145781 0.0 24 0.0 0.0 0.0 0.05209786956926226 0.0 25 0.0 0.0 0.0 0.05861010326542004 0.0 26 0.0 0.0 0.0 0.06419201786212671 0.0 27 0.0 0.0 0.0 0.06698297516048005 0.0 28 0.0 0.0 0.0 0.07814680435389339 0.0 29 0.0 0.0 0.0 0.08093776165224673 0.0 30 0.0 0.0 0.0 0.08558935714950228 0.0 31 0.0 0.0 0.0 0.08838031444785562 0.0 32 0.0 0.0 0.0 0.09768350544236673 0.0 33 0.0 0.0 0.0 0.10419573913852452 0.0 34 0.0 0.0 0.0 0.11628988743138896 0.0 35 0.0 0.0 0.0 0.15536328960833565 0.0 36 0.0 0.0 0.0 0.21211275467485347 0.0 37 0.0 0.0 0.0 0.2939808354265513 0.0 38 0.0 0.0 0.0 0.35538189599032466 0.0 39 0.0 0.0 0.0 0.44748348683598477 0.0 40 0.0 0.0 0.0 0.4977207182063448 0.0 41 0.0 0.0 0.0 0.6019164573448693 0.0 42 0.0 0.0 0.0 0.691227090892176 0.0 43 0.0 0.0 0.0 0.8168201693180761 0.0 44 0.0 0.0 0.0 0.9098520792631872 0.0 45 0.0 0.0 0.0 1.007535584705554 0.0 46 0.0 0.0 0.0 1.1163829193413342 0.0 47 0.0 0.0 0.0 1.2633733370546096 0.0 48 0.0 0.0 0.0 1.399199925574472 0.0 49 0.0 0.0 0.0 1.4894408782212298 0.0 50 0.0 0.0 0.0 1.6020094892548145 0.0 51 0.0 0.0 0.0 1.7015536328960834 0.0 52 0.0 0.0 0.0 1.7964461810400967 0.0 53 0.0 0.0 0.0 1.898781281979719 0.0 54 0.0 0.0 0.0 1.9825100009303191 0.0 55 0.0 0.0 0.0 2.031816913201228 0.0 56 0.0 0.0 0.0 2.1350823332403013 0.0 57 0.0 0.0 0.0 2.2104381802958413 0.0 58 0.0 0.0 0.0 2.3044004093404036 0.0 59 0.0 0.0 0.0 2.354637640710764 0.0 60 0.0 0.0 0.0 2.4253418922690484 0.0 61 0.0 0.0 0.0 2.489533910131175 0.0 62 0.0 0.0 0.0 2.5658200762861663 0.0 63 0.0 0.0 0.0 2.6458275188389617 0.0 64 0.0 0.0 0.0 2.7109498558005396 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGCA 15 0.0022157175 70.029335 31 ACCCGTG 35 3.6598067E-9 70.029335 27 ACCCGGT 15 0.0022157175 70.029335 27 CCCGTGC 30 1.01252226E-7 70.029335 28 CAGGGAG 15 0.0022157175 70.029335 29 AGCGCAT 15 0.0022157175 70.029335 32 GTGCGCA 50 0.0 70.029335 31 GGGCGAT 15 0.0022157175 70.029335 31 GACCCGC 15 0.0022157175 70.029335 26 AACGGGC 15 0.0022157175 70.029335 28 GACCAGT 15 0.0022157175 70.029335 26 TGCGCAT 40 1.3278623E-10 70.02933 32 GCGCGCA 20 7.8796234E-5 70.02933 31 CCGAGCG 20 7.8796234E-5 70.02933 29 CGCGCAT 20 7.8796234E-5 70.02933 32 CTCGAAA 15 0.0022198139 69.99674 39 ATCCCAC 15 0.0022198139 69.99674 17 ACTGCAT 15 0.0022198139 69.99674 53 AATCCGA 15 0.0022198139 69.99674 11 TCCCACG 15 0.0022198139 69.99674 18 >>END_MODULE