FastQCFastQC Report
Wed 25 May 2016
SRR1780146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239804
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9546839.81084552384447No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG74723.1158779670063885No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT6450.2689696585544862No Hit
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG4930.20558456072459175No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC4100.1709729612516889Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAATT150.00222343569.99457622
TGTGCGC252.388124E-455.9956633
GCCGTAT9400.054.3574954
TCGCCGT9500.053.7853152
GATCTCG9650.053.3119341
TGGTCGC9650.053.3119349
CCGTATC9600.053.22504455
GTCGCCG9750.053.12409251
GGTGGTC9750.053.12409247
TCTCGGT9900.053.0261943
TCGGTGG9900.053.0261945
GTAGATC9800.052.86407538
CGCCGTA9750.052.7651553
CTCGGTG9900.052.67268444
AGATCTC9850.052.5847640
ATCTCGG9800.052.49593742
GATACTA200.00694749252.4959341
GTACCAG200.00694749252.4959341
CATTGTG200.00694749252.49593415
GTCTCGG200.00694749252.49593442