FastQCFastQC Report
Wed 25 May 2016
SRR1780146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239804
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT3419114.257893946723158No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT2972612.395956697970009No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113094.715934679988657No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG47311.9728611699554637No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC20050.8360994812430151No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC18000.7506130006171707No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT15850.6609564477656753No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT14040.5854781404813931No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT10370.4324364898000034No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT8470.35320511751263534No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC7000.29190505579556636No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT6070.25312338409701257No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT5750.23977915297492955No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCTT5240.2185117846241097RNA PCR Primer, Index 26 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT4640.193491351270204No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCG3240.13511034011109072No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGA2900.1209320945438775No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATTCCGT2780.11592800787309636No Hit
CTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC2690.11217494287001052No Hit
CAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC2410.10049874063818787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCT150.002221648670.0087632
CGTTCTA207.910942E-570.0087628
AACGAGT150.002221648670.0087630
TCGAGTC150.002221648670.0087631
TCCGATC150.00222348769.9941613
GCCGAAA252.8355425E-669.9941646
ACGTATG301.0214171E-769.9941640
ATATACA45800.067.625362
CATATAC45700.067.6201861
AGGGGGG72550.066.5209870
CAAATAC3850.065.449081
CGAATGC700.064.99457641
ATATCCG1250.064.3946310
TGACGTA600.064.16131645
CGTTAGA550.063.6443228
CCGACTT453.8380676E-1062.21703347
CTTAATA401.0630174E-861.244892
GACGTAT401.0630174E-861.2448946
CCGAAAT401.0630174E-861.2448947
AAGCTTG2850.060.1704254
Produced by FastQC (version 0.11.5)