FastQCFastQC Report
Wed 25 May 2016
SRR1780145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154079
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9525861.82412918048533No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG69524.511971131692183No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT5970.3874635738809312No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC4390.28491877543338157Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG4380.2842697577216882No Hit
TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG1990.12915452462697707No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1920.1246114006451236No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA150.002221238870.01
CGACGAC150.002221238870.022
GGTCAGT150.002221238870.050
TGTGCGC353.6852725E-970.03
TTTATAC207.906877E-570.03
CTTTATA207.906877E-570.02
ATGTTAA150.002221238870.01
CTCTAGT150.002221238870.01
GCATCCG150.002221238870.021
TGCGCGT455.456968E-1269.999995
AAAAAGG8250.058.54545270
CGCTGGC308.345836E-658.33333216
CTATACA252.3845205E-455.9999961
TGTGCGG252.3845205E-455.9999963
GTGTGCG252.3845205E-455.9999962
TTGTGCG452.6830094E-854.4444432
GCCGTAT10450.053.92344754
CGCCGTA10500.053.66666853
TCTGGCG850.053.5294111
TCGCCGT10400.053.50961352