##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780145_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 154079 Sequences flagged as poor quality 0 Sequence length 76 %GC 82 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.89994742956535 32.0 27.0 32.0 22.0 32.0 2 29.658856820202622 32.0 32.0 32.0 22.0 32.0 3 29.537159509083004 32.0 32.0 32.0 22.0 32.0 4 28.995567209029133 32.0 32.0 32.0 13.0 32.0 5 29.681286872318747 32.0 32.0 32.0 22.0 32.0 6 33.84371653502424 37.0 37.0 37.0 22.0 37.0 7 33.47449684901901 37.0 37.0 37.0 22.0 37.0 8 33.79526736284633 37.0 37.0 37.0 22.0 37.0 9 33.91210353130537 37.0 37.0 37.0 22.0 37.0 10-11 33.73841016621344 37.0 37.0 37.0 22.0 37.0 12-13 33.52095353682202 37.0 37.0 37.0 22.0 37.0 14-15 34.14688568851044 37.0 37.0 37.0 27.0 37.0 16-17 34.34415786706819 37.0 37.0 37.0 27.0 37.0 18-19 33.84253856787752 37.0 37.0 37.0 24.5 37.0 20-21 34.32120860078271 37.0 37.0 37.0 27.0 37.0 22-23 34.4031633123268 37.0 37.0 37.0 29.5 37.0 24-25 33.94443434861337 37.0 37.0 37.0 22.0 37.0 26-27 34.3783870611829 37.0 37.0 37.0 29.5 37.0 28-29 34.1587886733429 37.0 37.0 37.0 27.0 37.0 30-31 34.09097605773661 37.0 37.0 37.0 24.5 37.0 32-33 33.960361243258326 37.0 37.0 37.0 24.5 37.0 34-35 34.07489015375229 37.0 37.0 37.0 22.0 37.0 36-37 34.79388820020898 37.0 37.0 37.0 29.5 37.0 38-39 34.95031444908132 37.0 37.0 37.0 29.5 37.0 40-41 34.50348522511179 37.0 37.0 37.0 29.5 37.0 42-43 34.38946579352151 37.0 37.0 37.0 27.0 37.0 44-45 34.24214526314423 37.0 37.0 37.0 27.0 37.0 46-47 34.475765678645374 37.0 37.0 37.0 27.0 37.0 48-49 34.93845040531156 37.0 37.0 37.0 32.0 37.0 50-51 35.18897773220231 37.0 37.0 37.0 32.0 37.0 52-53 34.417587730969174 37.0 37.0 37.0 27.0 37.0 54-55 34.576003219127855 37.0 37.0 37.0 29.5 37.0 56-57 34.464229388820016 37.0 37.0 37.0 29.5 37.0 58-59 34.15540080088786 37.0 37.0 37.0 27.0 37.0 60-61 33.961665768858836 37.0 37.0 37.0 24.5 37.0 62-63 33.63321737550218 37.0 37.0 37.0 22.0 37.0 64-65 33.470151675439226 37.0 37.0 37.0 17.5 37.0 66-67 33.551473594714395 37.0 37.0 37.0 22.0 37.0 68-69 33.698388489021866 37.0 37.0 37.0 22.0 37.0 70-71 33.8060053608863 37.0 37.0 37.0 22.0 37.0 72-73 34.009352345225494 37.0 37.0 37.0 27.0 37.0 74-75 33.796678976369265 37.0 37.0 37.0 22.0 37.0 76 34.466338696383026 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.7629464525990208 11101 2 0.045128877798756406 11101 3 0.26626433787536286 11101 4 0.08009355573210541 11101 5 -0.073163824297648 11101 6 0.1796954752226867 11101 7 -1.1318468292355988 11101 8 -0.28236842116051264 11101 9 -0.5799847955783406 11101 10-11 -1.1031503184703269 11101 12-13 -0.21868063020494333 11101 14-15 -0.49516112131253465 11101 16-17 -0.5039667910796126 11101 18-19 -0.5984948273456396 11101 20-21 -1.1476937124117015 11101 22-23 -0.4514285597696528 11101 24-25 -0.3852943499403523 11101 26-27 0.6353853098438975 11101 28-29 0.36599245586526763 11101 30-31 -0.7879203407350701 11101 32-33 -0.6279944933066801 11101 34-35 -0.8781348554264028 11101 36-37 0.08124609244434566 11101 38-39 -0.1923805622571635 11101 40-41 -0.09957666313557212 11101 42-43 -0.09339850808353134 11101 44-45 -0.7430052813776413 11101 46-47 -0.7541342471402785 11101 48-49 -0.9383367932302207 11101 50-51 -1.2204218893402867 11101 52-53 -1.6029905792359216 11101 54-55 -0.2631364242584695 11101 56-57 -0.9430655454939227 11101 58-59 -1.8625825853438656 11101 60-61 -1.2614316478693581 11101 62-63 -2.0300007569854124 11101 64-65 -1.0717009049171438 11101 66-67 -0.6548563874700406 11101 68-69 -1.0732336548132366 11101 70-71 -1.0445834225491097 11101 72-73 -0.49263341547945316 11101 74-75 -1.5555861702515656 11101 76 -1.459101486880833 11102 1 -0.1927632111829034 11102 2 -0.4787992488690982 11102 3 -1.003260657677032 11102 4 -2.0511998084379712 11102 5 -1.2069478484926321 11102 6 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46-47 0.26633441463273044 11103 48-49 -0.318771575838916 11103 50-51 -0.23714972672029688 11103 52-53 -0.544054949648114 11103 54-55 0.8068099337313654 11103 56-57 0.5080214110278121 11103 58-59 0.703905274622258 11103 60-61 0.41106976376248383 11103 62-63 -0.14942481119206974 11103 64-65 -0.9605348970120104 11103 66-67 0.748093923088966 11103 68-69 0.32378780199082513 11103 70-71 0.6194481980042497 11103 72-73 0.35293406052280574 11103 74-75 0.06242625210868624 11103 76 0.26675960854547753 11104 1 -0.9887906084431783 11104 2 -0.973182810512931 11104 3 -1.3349044932934682 11104 4 -0.9338025481639995 11104 5 -1.0616053827392058 11104 6 -0.874330498803289 11104 7 -2.299509166897934 11104 8 -1.0899008886929806 11104 9 -1.6876471332406808 11104 10-11 -1.8175659028859137 11104 12-13 -1.0414079029322139 11104 14-15 -1.1630182641696791 11104 16-17 -1.6156551027679242 11104 18-19 -1.7907675546183626 11104 20-21 -1.1985378682558476 11104 22-23 -1.1414934948345916 11104 24-25 -0.9037359083819112 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21611 44-45 0.20709091871856344 21611 46-47 1.362147619141588 21611 48-49 0.6264780215845889 21611 50-51 1.261239967321572 21611 52-53 0.6751479489026053 21611 54-55 1.3343864232643838 21611 56-57 0.9921196396912606 21611 58-59 0.3220614743001917 21611 60-61 0.8271325656948534 21611 62-63 0.780124303139651 21611 64-65 0.6286357954195552 21611 66-67 0.5415721839585288 21611 68-69 0.5609053543257758 21611 70-71 0.5985142205485303 21611 72-73 0.5036989558529186 21611 74-75 -0.2586284982938878 21611 76 0.30580952803018135 21612 1 1.6890525288117253 21612 2 1.1705426796831446 21612 3 0.8664680557261377 21612 4 1.407570000855607 21612 5 1.133100499613736 21612 6 1.8135126384516127 21612 7 1.471667303690296 21612 8 0.8089226407187908 21612 9 1.1313724746024576 21612 10-11 1.057532136329776 21612 12-13 1.930160724518764 21612 14-15 0.7786484024969909 21612 16-17 0.9147370540948216 21612 18-19 1.443840288518885 21612 20-21 1.4513640925284648 21612 22-23 1.2703692502634496 21612 24-25 1.51704840534358 21612 26-27 1.141751492680669 21612 28-29 1.1601101029240937 21612 30-31 0.23983621643325392 21612 32-33 0.3360365736655666 21612 34-35 0.8992397307717184 21612 36-37 0.6149231996508604 21612 38-39 0.7733693740809997 21612 40-41 0.9567640549698453 21612 42-43 1.0013939543559829 21612 44-45 1.6030604176292371 21612 46-47 1.072387270557705 21612 48-49 0.4195063440246827 21612 50-51 0.8916865904152544 21612 52-53 0.7829910861575087 21612 54-55 0.5462600616086064 21612 56-57 0.8118364152903865 21612 58-59 0.36971942636991173 21612 60-61 0.9161848532765617 21612 62-63 0.7855709262333264 21612 64-65 0.5414898259206424 21612 66-67 1.2842192427820578 21612 68-69 0.587321476330132 21612 70-71 1.1203007129232603 21612 72-73 1.497435339295187 21612 74-75 0.4437135496363922 21612 76 0.3673563689887871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 6.0 12 46.0 13 81.0 14 154.0 15 298.0 16 578.0 17 850.0 18 1074.0 19 1095.0 20 1121.0 21 1208.0 22 1347.0 23 1505.0 24 1744.0 25 1885.0 26 2099.0 27 2387.0 28 2457.0 29 2968.0 30 3473.0 31 4276.0 32 5373.0 33 7620.0 34 12584.0 35 23817.0 36 74029.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 76.65937603437197 6.059229356369135 3.758461568416202 13.522933040842686 2 71.5118316696738 6.165708277625618 15.950362803255494 6.372097249445085 3 71.1414274495551 6.980185489262001 15.345374775277618 6.533012285905282 4 71.13956748617566 10.99587216698253 10.37280303226979 7.4917573145720295 5 70.47942938362787 9.903361262728861 16.055400151870145 3.561809201773116 6 74.75369617985696 15.677773595192047 5.447890029725204 4.120640195225795 7 73.3571739172762 9.078459751166609 6.21304655404046 11.351319777516728 8 73.93804476924176 15.00334244121522 5.686693189857151 5.371919599685875 9 72.4881392013188 5.712004880613192 5.255745429292765 16.54411048877524 10-11 74.16390293291104 10.077947027174371 9.934838621745987 5.823311418168601 12-13 73.2064279131349 6.967250353716949 10.207167798128221 9.619153935019925 14-15 76.99037506733559 9.076188189175681 6.506078050870008 7.427358692618721 16-17 76.62944538011467 7.574061273961 6.4003089334332826 9.396184412491035 18-19 73.13737007265871 7.147099006013247 9.6860974905323 10.029433430795741 20-21 76.80325554679361 6.8797440215998 6.980344052104326 9.336656379502257 22-23 76.86463998753878 6.794935032905411 6.312062721478731 10.028362258077077 24-25 74.22871106236187 10.207916197473335 6.122283411162637 9.441089329002152 26-27 76.69062647044507 10.245817851405949 6.442537034933718 6.621018643215265 28-29 74.0390387954114 10.652431406272818 6.791062939105321 8.517466859210462 30-31 73.8535727382177 10.51652912676495 9.336595781965453 6.293302353051892 32-33 74.05189815085252 12.958635953553882 7.155791810269291 5.833674085324299 34-35 79.56327314736691 7.857708433390879 6.952971871389815 5.626046547852386 36-37 77.69708298042882 6.781932586311523 9.995229704339021 5.5257547289206475 38-39 77.35013889249461 6.904384849035542 10.148887561982397 5.596588696487448 40-41 76.96718545152456 9.54743701242228 7.591933955529018 5.89344358052415 42-43 74.48857072391905 9.738573969028675 9.798283986033047 5.974571321019224 44-45 73.93374156679874 6.756945323325447 10.433642591276525 8.875670518599286 46-47 76.44269929094125 6.65606594084146 7.725657542470509 9.175577225746784 48-49 77.25777036455325 6.5193829139597215 10.113643001317506 6.109203720169523 50-51 79.93808330845415 6.294863640493775 7.679551915263698 6.087501135788367 52-53 74.12349589169122 6.70569451836083 10.158491153831177 9.012318436116772 54-55 76.39052947208556 6.868923532236919 7.456937395345214 9.283609600332298 56-57 76.41307381278435 6.888673991913239 7.039570609881944 9.658681585420466 58-59 73.98030212068602 9.577972124417906 9.942074605312262 6.499651149583813 60-61 73.26702471158994 7.42710648861774 10.424623971702554 8.88124482808976 62-63 73.26377959143937 9.940452045236976 10.076422579546007 6.719345783777644 64-65 73.35723905177589 10.182538008469763 10.255877723872727 6.204345215881618 66-67 73.27732237777215 13.738909765896274 6.6885388474593865 6.295229008872187 68-69 73.27109233280437 14.090220244875177 6.3152873372988445 6.323400085021596 70-71 73.54692575537794 14.065882001706923 6.191973571913018 6.195218671002118 72-73 74.20186010890659 13.384541495486024 6.1706159907059455 6.242982404901445 74-75 76.49943209475904 11.216290767483368 6.225864027259451 6.058413110498134 76 80.61708604027804 7.085975376268018 6.35842652145977 5.938512061994172 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.5 10 3.0 11 4.0 12 4.0 13 4.0 14 4.0 15 3.5 16 6.5 17 10.0 18 20.5 19 31.0 20 35.5 21 40.0 22 67.0 23 98.5 24 116.5 25 130.0 26 184.0 27 224.0 28 305.0 29 400.0 30 431.5 31 511.0 32 644.0 33 729.0 34 817.5 35 1024.5 36 1230.0 37 1317.0 38 1454.0 39 1736.5 40 2013.5 41 2400.5 42 2656.0 43 3793.0 44 6677.0 45 5704.5 46 2985.0 47 2815.5 48 2404.5 49 2042.5 50 1922.0 51 1708.5 52 1390.0 53 1207.0 54 1129.0 55 1090.5 56 991.5 57 771.5 58 612.0 59 618.5 60 564.5 61 466.5 62 429.0 63 414.5 64 364.0 65 327.0 66 306.0 67 286.0 68 276.0 69 250.0 70 234.5 71 235.0 72 222.0 73 190.0 74 180.0 75 189.0 76 172.5 77 162.5 78 168.5 79 168.0 80 150.5 81 150.0 82 178.5 83 190.0 84 177.0 85 179.5 86 197.5 87 200.0 88 214.0 89 272.5 90 333.0 91 378.5 92 408.0 93 471.5 94 579.5 95 750.0 96 876.0 97 1092.5 98 2010.0 99 49707.5 100 96704.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 6.490177116933521E-4 3 0.0 4 0.0019470531350800563 5 0.0 6 6.490177116933521E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.490177116933521E-4 14-15 0.0 16-17 3.2450885584667607E-4 18-19 0.0016225442792333802 20-21 0.0022715619909267322 22-23 6.490177116933521E-4 24-25 0.0016225442792333802 26-27 9.735265675400282E-4 28-29 9.735265675400282E-4 30-31 0.0016225442792333802 32-33 0.005192141693546817 34-35 6.490177116933521E-4 36-37 3.2450885584667607E-4 38-39 0.0019470531350800563 40-41 6.490177116933521E-4 42-43 6.490177116933521E-4 44-45 3.2450885584667607E-4 46-47 9.735265675400282E-4 48-49 0.0 50-51 6.490177116933521E-4 52-53 6.490177116933521E-4 54-55 6.490177116933521E-4 56-57 0.0 58-59 9.735265675400282E-4 60-61 9.735265675400282E-4 62-63 9.735265675400282E-4 64-65 9.735265675400282E-4 66-67 0.0012980354233867043 68-69 3.2450885584667607E-4 70-71 3.2450885584667607E-4 72-73 0.0012980354233867043 74-75 0.0025960708467734086 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 154079.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.08552755404695 #Duplication Level Percentage of deduplicated Percentage of total 1 73.54482164645087 13.300968983443559 2 9.377018588961459 3.391766561309458 3 6.215459699992823 3.3722960299586573 4 4.119715782674227 2.980289332095873 5 2.576616665470466 2.329973584979134 6 1.6005167587741331 1.7367713964914104 7 0.9725113040981842 1.231186599082289 8 0.47369554295557315 0.6853627035481799 9 0.2834996052537142 0.46145159301397337 >10 0.7500179430129907 2.0632273054731662 >50 0.04665183377592765 0.6048845072982042 >100 0.028708820785186245 1.1182575172476457 >500 0.0035886025981482807 0.3874635738809312 >1k 0.0 0.0 >5k 0.0035886025981482807 4.511971131692183 >10k+ 0.0035886025981482807 61.82412918048533 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 95258 61.82412918048533 No Hit CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG 6952 4.511971131692183 No Hit TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT 597 0.3874635738809312 No Hit ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC 439 0.28491877543338157 Illumina Single End PCR Primer 1 (95% over 22bp) CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG 438 0.2842697577216882 No Hit TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG 199 0.12915452462697707 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 192 0.1246114006451236 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.014927407368947099 0.0 2 0.0 0.0 0.0 0.018172495927413858 0.0 3 0.0 0.0 0.0 0.018172495927413858 0.0 4 0.0 0.0 0.0 0.03439793871974766 0.0 5 0.0 0.0 0.0 0.03699400956652107 0.0 6 0.0 0.0 0.0 0.03829204498990778 0.0 7 0.0 0.0 0.0 0.03829204498990778 0.0 8 0.0 0.0 0.0 0.03829204498990778 0.0 9 0.0 0.0 0.0 0.04283516897176124 0.0 10 0.0 0.0 0.0 0.048027310665308055 0.0 11 0.0 0.0 0.0 0.04867632837700141 0.0 12 0.0 0.0 0.0 0.04867632837700141 0.0 13 0.0 0.0 0.0 0.04867632837700141 0.0 14 0.0 0.0 0.0 0.05386847007054823 0.0 15 0.0 0.0 0.0 0.05386847007054823 0.0 16 0.0 0.0 0.0 0.055815523205628284 0.0 17 0.0 0.0 0.0 0.0623057003225618 0.0 18 0.0 0.0 0.0 0.06490177116933521 0.0 19 0.0 0.0 0.0 0.06944489515118868 0.0 20 0.0 0.0 0.0 0.07204096599796209 0.0 21 0.0 0.0 0.0 0.0759350722681222 0.0 22 0.0 0.0 0.0 0.08242524938505572 0.0 23 0.0 0.0 0.0 0.08567033794352248 0.0 24 0.0 0.0 0.0 0.09410756819553606 0.0 25 0.0 0.0 0.0 0.09475658590722941 0.0 26 0.0 0.0 0.0 0.09475658590722941 0.0 27 0.0 0.0 0.0 0.1057898870060164 0.0 28 0.0 0.0 0.0 0.11422711725802998 0.0 29 0.0 0.0 0.0 0.12850550691528373 0.0 30 0.0 0.0 0.0 0.14278389657253746 0.0 31 0.0 0.0 0.0 0.1570622862297912 0.0 32 0.0 0.0 0.0 0.18107594156244525 0.0 33 0.0 0.0 0.0 0.20508959689509926 0.0 34 0.0 0.0 0.0 0.22780521680436658 0.0 35 0.0 0.0 0.0 0.2641502086591943 0.0 36 0.0 0.0 0.0 0.33424412152207633 0.0 37 0.0 0.0 0.0 0.46923980555429357 0.0 38 0.0 0.0 0.0 0.6178648615320712 0.0 39 0.0 0.0 0.0 0.8106231219049967 0.0 40 0.0 0.0 0.0 1.0033813822779223 0.0 41 0.0 0.0 0.0 1.1825102707052875 0.0 42 0.0 0.0 0.0 1.430435036572148 0.0 43 0.0 0.0 0.0 1.6218952615216868 0.0 44 0.0 0.0 0.0 1.8354220886687997 0.0 45 0.0 0.0 0.0 2.117744793255408 0.0 46 0.0 0.0 0.0 2.3377617975194545 0.0 47 0.0 0.0 0.0 2.5837395102512346 0.0 48 0.0 0.0 0.0 2.75897429240844 0.0 49 0.0 0.0 0.0 2.9238247911785513 0.0 50 0.0 0.0 0.0 3.0938674316422095 0.0 51 0.0 0.0 0.0 3.2639100721058676 0.0 52 0.0 0.0 0.0 3.46380752730742 0.0 53 0.0 0.0 0.0 3.6585128408154257 0.0 54 0.0 0.0 0.0 3.846078959494805 0.0 55 0.0 0.0 0.0 4.013525529111689 0.0 56 0.0 0.0 0.0 4.183568169575348 0.0 57 0.0 0.0 0.0 4.366591164272873 0.0 58 0.0 0.0 0.0 4.517163273385731 0.0 59 0.0 0.0 0.0 4.705378409776803 0.0 60 0.0 0.0 0.0 4.859195607448127 0.0 61 0.0 0.0 0.0 5.014959858254532 0.0 62 0.0 0.0 0.0 5.174618215331097 0.0 63 0.0 0.0 0.0 5.341415767236288 0.0 64 0.0 0.0 0.0 5.494583947195919 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAATA 15 0.0022212388 70.0 1 CGACGAC 15 0.0022212388 70.0 22 GGTCAGT 15 0.0022212388 70.0 50 TGTGCGC 35 3.6852725E-9 70.0 3 TTTATAC 20 7.906877E-5 70.0 3 CTTTATA 20 7.906877E-5 70.0 2 ATGTTAA 15 0.0022212388 70.0 1 CTCTAGT 15 0.0022212388 70.0 1 GCATCCG 15 0.0022212388 70.0 21 TGCGCGT 45 5.456968E-12 69.99999 5 AAAAAGG 825 0.0 58.545452 70 CGCTGGC 30 8.345836E-6 58.333332 16 CTATACA 25 2.3845205E-4 55.999996 1 TGTGCGG 25 2.3845205E-4 55.999996 3 GTGTGCG 25 2.3845205E-4 55.999996 2 TTGTGCG 45 2.6830094E-8 54.444443 2 GCCGTAT 1045 0.0 53.923447 54 CGCCGTA 1050 0.0 53.666668 53 TCTGGCG 85 0.0 53.52941 11 TCGCCGT 1040 0.0 53.509613 52 >>END_MODULE