FastQCFastQC Report
Wed 25 May 2016
SRR1780145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154079
Sequences flagged as poor quality0
Sequence length76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT3736424.24989777971041No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT3224620.928225131263833No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105126.822474185320518No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG52813.4274625354525927No Hit
CAAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT23731.5401190298483245No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC21151.3726724602314397No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC18821.2214513334068886No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC9070.5886590645058704No Hit
CTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT8370.5432278246873358No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCTT8120.5270023818950019RNA PCR Primer, Index 39 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACGT4310.27972663373983475No Hit
AAACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCTT3820.2479247658668605RNA PCR Primer, Index 39 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC3620.23494441163299348No Hit
CAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC3130.20314254376001922No Hit
CATAAACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2590.1680955873285782No Hit
TCATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG2410.15641326851809786No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG2240.14537996741931086No Hit
CATATACAAATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2240.14537996741931086No Hit
CATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTAAGCCGT2090.13564470174391058No Hit
CTTTACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC2050.13304863089713717No Hit
ACTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG2000.12980354233867042No Hit
CATATACACATCTCCGAGCCCACGAGACGCATGTACATCTAGTATGCCGT1870.12136631208665685No Hit
CATTACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC1820.11812122352819007No Hit
CATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG1780.11552515268141667No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACGT1760.11422711725802998No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC1710.11098202869956322No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG1700.11033301098786986No Hit
CATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCAGT1680.10903497556448316No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCC150.002216582770.03702524
GGTCACC150.002216582770.03702531
ATGTTCT150.002216582770.03702524
GCCGACA353.6889105E-969.9915746
TCACGAA150.00222230169.9915638
CAGGTAT150.00222230169.991561
AGCCGAC150.00222230169.9915645
ACTGACC150.00222230169.991566
GACGTAT150.00222230169.9915646
CGTCTAA150.00222230169.9915648
CTGACCT150.00222230169.991567
CCGTCAA150.00222230169.9915647
TGACCTT150.00222230169.991568
TAACATC150.00222230169.9915656
ATATACG150.00222230169.9915610
ATGACGA301.0192525E-769.9915644
ACGTATT150.00222230169.9915647
ACTCCGA150.00222230169.9915611
ATACGAG150.00222230169.9915612
CCTATGC150.00222230169.9915641
Produced by FastQC (version 0.11.5)