FastQCFastQC Report
Wed 25 May 2016
SRR1780144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177181
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10099557.0010328421219No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG67613.8158719050011003No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT6010.33920115588014516No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC4150.23422375988396046Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG3820.21559873801366963No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2150.12134483945795542No Hit
TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG1960.11062134201748496No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACA150.002222039369.99802436
TATGCGC150.002222039369.9980243
TCGTGCT150.002222039369.99802447
TACTGTA150.002222039369.9980245
GGGCCCC150.002222039369.99802469
TGTGCGC401.0604708E-861.2482723
CTTTATA401.0604708E-861.2482722
TGCGGGT352.962097E-759.998315
GCGCGTC553.6379788E-1157.2711076
CGCGTCT553.6379788E-1157.2711077
GGGCGCT252.3858332E-455.9984213
GATTGGC252.3858332E-455.9984210
TATACGC252.3858332E-455.998423
TTATATC200.00693540852.5133430
CGGCGTG200.006943157452.49851653
CGTGCTC200.006943157452.49851648
CTTTTCT200.006943157452.49851637
ATGTGAC200.006943157452.49851640
TCGGCGT200.006943157452.49851652
TGTGACA200.006943157452.49851641