##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780144_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 177181 Sequences flagged as poor quality 0 Sequence length 76 %GC 79 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.086171767853212 32.0 27.0 32.0 22.0 32.0 2 29.713456860498585 32.0 32.0 32.0 22.0 32.0 3 29.59037368566607 32.0 32.0 32.0 22.0 32.0 4 29.016830247035518 32.0 32.0 32.0 13.0 32.0 5 29.68557012320734 32.0 32.0 32.0 22.0 32.0 6 33.83567086764382 37.0 37.0 37.0 22.0 37.0 7 33.55262697467561 37.0 37.0 37.0 22.0 37.0 8 33.75960176316874 37.0 37.0 37.0 22.0 37.0 9 33.9534713090004 37.0 37.0 37.0 22.0 37.0 10-11 33.745288716058724 37.0 37.0 37.0 22.0 37.0 12-13 33.53198424210271 37.0 37.0 37.0 22.0 37.0 14-15 34.18699804155073 37.0 37.0 37.0 27.0 37.0 16-17 34.35435515094734 37.0 37.0 37.0 27.0 37.0 18-19 33.862905729169604 37.0 37.0 37.0 24.5 37.0 20-21 34.29526303610432 37.0 37.0 37.0 27.0 37.0 22-23 34.37864387265 37.0 37.0 37.0 27.0 37.0 24-25 33.89793488015081 37.0 37.0 37.0 22.0 37.0 26-27 34.348821261873454 37.0 37.0 37.0 27.0 37.0 28-29 34.116457182203504 37.0 37.0 37.0 24.5 37.0 30-31 34.04742325644398 37.0 37.0 37.0 24.5 37.0 32-33 33.97028180222485 37.0 37.0 37.0 24.5 37.0 34-35 34.06101387846327 37.0 37.0 37.0 22.0 37.0 36-37 34.75145190511398 37.0 37.0 37.0 29.5 37.0 38-39 34.8554077468803 37.0 37.0 37.0 29.5 37.0 40-41 34.43950536457069 37.0 37.0 37.0 29.5 37.0 42-43 34.27909030878028 37.0 37.0 37.0 27.0 37.0 44-45 34.151977356488565 37.0 37.0 37.0 27.0 37.0 46-47 34.40674507989006 37.0 37.0 37.0 27.0 37.0 48-49 34.832408666843506 37.0 37.0 37.0 29.5 37.0 50-51 35.08911508570332 37.0 37.0 37.0 32.0 37.0 52-53 34.28429967095795 37.0 37.0 37.0 27.0 37.0 54-55 34.46972869551475 37.0 37.0 37.0 29.5 37.0 56-57 34.35429588951411 37.0 37.0 37.0 29.5 37.0 58-59 34.060051585666635 37.0 37.0 37.0 24.5 37.0 60-61 33.843061614958714 37.0 37.0 37.0 22.0 37.0 62-63 33.555022829761654 37.0 37.0 37.0 22.0 37.0 64-65 33.40164577465981 37.0 37.0 37.0 17.5 37.0 66-67 33.46489747772053 37.0 37.0 37.0 22.0 37.0 68-69 33.626263538415515 37.0 37.0 37.0 22.0 37.0 70-71 33.725204734141926 37.0 37.0 37.0 22.0 37.0 72-73 33.90583358260761 37.0 37.0 37.0 22.0 37.0 74-75 33.77407848471337 37.0 37.0 37.0 22.0 37.0 76 34.26687963156321 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.6535926948625495 11101 2 0.4179682796618849 11101 3 -0.31221625242686457 11101 4 0.1941918349534042 11101 5 0.19816275198193622 11101 6 0.07559027677352503 11101 7 -0.23217637297942417 11101 8 0.2550605729788842 11101 9 0.010574019194343975 11101 10-11 -0.012862392859943839 11101 12-13 -0.06255488658084829 11101 14-15 0.20906360709982863 11101 16-17 -0.32760907535612205 11101 18-19 0.7953516849582911 11101 20-21 0.5743726352490981 11101 22-23 0.20840249283828882 11101 24-25 0.16767913162049553 11101 26-27 0.6478451556931333 11101 28-29 0.18022892301947735 11101 30-31 0.5280720722729271 11101 32-33 0.5809926490013098 11101 34-35 0.22586368286356873 11101 36-37 0.5127890827133825 11101 38-39 0.7540435376419339 11101 40-41 0.44249168055664967 11101 42-43 0.4330586254621167 11101 44-45 0.44872367471500496 11101 46-47 0.12518646110127918 11101 48-49 -0.0349034704377047 11101 50-51 0.5446570486785731 11101 52-53 0.19426030927269977 11101 54-55 0.6947383608249567 11101 56-57 0.290287312600249 11101 58-59 0.6298871919561151 11101 60-61 1.3149803355352248 11101 62-63 0.08494494500649807 11101 64-65 0.5711260341471771 11101 66-67 0.08289702661309661 11101 68-69 0.835843215381594 11101 70-71 0.27632843415097597 11101 72-73 1.0618715581653149 11101 74-75 0.23043178058520652 11101 76 0.09423878898291349 11102 1 0.23975550283347502 11102 2 0.1848207508621833 11102 3 -0.10039908408795739 11102 4 0.30991089849855413 11102 5 0.6643091659544353 11102 6 -0.08301247313357152 11102 7 0.3718370049469968 11102 8 -0.10205574065916778 11102 9 0.8869025216098052 11102 10-11 0.32099119316755775 11102 12-13 0.4316108510631551 11102 14-15 -0.020442152840722372 11102 16-17 0.2493883233804084 11102 18-19 0.8053851297743364 11102 20-21 0.00595940596630129 11102 22-23 0.2693463798691269 11102 24-25 0.34991621820540786 11102 26-27 -0.45694860127453296 11102 28-29 0.9968398483260543 11102 30-31 0.9898334992517448 11102 32-33 0.9034006757571618 11102 34-35 1.0647339912990859 11102 36-37 -0.1229968704638793 11102 38-39 0.29406583418597165 11102 40-41 0.00841512908878883 11102 42-43 -0.1072981192424578 11102 44-45 0.34047395342181375 11102 46-47 0.307572191313092 11102 48-49 0.04309727278042175 11102 50-51 -0.33683681977181124 11102 52-53 -0.1412655175574713 11102 54-55 0.009119631727962485 11102 56-57 0.3476265916786545 11102 58-59 0.26652784598807955 11102 60-61 0.6991497892698959 11102 62-63 0.08223219881549682 11102 64-65 0.19468604901154407 11102 66-67 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46-47 -0.054300718385903224 11103 48-49 0.410823025288785 11103 50-51 0.10747756149908838 11103 52-53 0.7177645827769723 11103 54-55 -0.10141548532889288 11103 56-57 0.5125095348224775 11103 58-59 -0.061565799496875684 11103 60-61 0.232929053483943 11103 62-63 -0.016764456702901498 11103 64-65 0.774117487138632 11103 66-67 1.1273414710575338 11103 68-69 0.9328517623901433 11103 70-71 0.850260058082597 11103 72-73 0.27298266927643056 11103 74-75 0.9090642592176863 11103 76 0.6651789599230824 11104 1 0.14424026832474723 11104 2 -0.24297794821347551 11104 3 -0.3735931956935694 11104 4 -0.10926061810534904 11104 5 -0.7863584136415938 11104 6 -0.3008145956943409 11104 7 -0.5180637139848798 11104 8 -2.0335860385023743 11104 9 -0.4767400665449344 11104 10-11 -0.15826248707892887 11104 12-13 -0.1818092105595781 11104 14-15 -0.44081188923236425 11104 16-17 -0.33369966018685915 11104 18-19 -0.5059808407836854 11104 20-21 -0.9465910904390356 11104 22-23 -0.48091038151395793 11104 24-25 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11105 64-65 -0.16787234121483152 11105 66-67 0.18404840049173998 11105 68-69 -0.5208354594789029 11105 70-71 -0.35848120065865885 11105 72-73 -0.2745697782760175 11105 74-75 -0.38756638286750444 11105 76 0.30777272978958337 11106 1 0.1173474760776223 11106 2 -0.19143342973982413 11106 3 0.23906579885518653 11106 4 -0.14426970350813306 11106 5 0.31782087164005546 11106 6 0.34909455027779757 11106 7 0.383208242405189 11106 8 0.46617168408999277 11106 9 -0.955237946617622 11106 10-11 -0.05559743559498287 11106 12-13 -0.5040078780338462 11106 14-15 -0.4674321193959017 11106 16-17 -0.03572873347577854 11106 18-19 0.5684286080352052 11106 20-21 -0.04955898868252717 11106 22-23 0.1695136039493974 11106 24-25 0.20058511452648276 11106 26-27 0.36194771979570106 11106 28-29 0.448177640968197 11106 30-31 0.5614054056062656 11106 32-33 -0.07669965869099116 11106 34-35 1.386974793974673 11106 36-37 0.3166352365595344 11106 38-39 -0.02031543671704128 11106 40-41 -0.5904143023493305 11106 42-43 0.08434067674416923 11106 44-45 0.04231341830475088 11106 46-47 0.3687762046910237 11106 48-49 -0.3930231285573669 11106 50-51 0.415169869191395 11106 52-53 -0.09762003260764374 11106 54-55 0.5101229762095727 11106 56-57 0.27020184251477986 11106 58-59 0.24612650819543802 11106 60-61 0.5521598227147138 11106 62-63 0.2678509279124839 11106 64-65 0.039502102523250926 11106 66-67 0.42734147105753095 11106 68-69 0.2174671470055216 11106 70-71 -0.02666301884048039 11106 72-73 -0.3116327153389591 11106 74-75 0.35906425921768914 11106 76 0.5497943445384692 11107 1 -0.3042999030918807 11107 2 -0.5344558940012263 11107 3 -0.7040928124450154 11107 4 -0.16319414190159875 11107 5 0.16465545298791184 11107 6 0.14215105129890304 11107 7 -0.2859544533061822 11107 8 -0.1886274990277741 11107 9 -0.1615007104025068 11107 10-11 -1.0545763328039683 11107 12-13 -0.5567308188098821 11107 14-15 -0.4129392671154406 11107 16-17 -0.24610994518442197 11107 18-19 -0.0957306840001877 11107 20-21 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0.5678678322841151 21611 22-23 0.3855449177683212 21611 24-25 0.7908165644924381 21611 26-27 -0.04332797795787258 21611 28-29 -0.2417134414007691 21611 30-31 -0.06303298785867639 21611 32-33 0.595628455672518 21611 34-35 0.7474581159624236 21611 36-37 0.43995041695436043 21611 38-39 -0.3547606375339214 21611 40-41 0.589946487303493 21611 42-43 0.6296776406652 21611 44-45 -0.0016961119879610465 21611 46-47 0.30614856684213976 21611 48-49 0.14155821929831092 21611 50-51 0.007103157142381633 21611 52-53 0.06442898032633337 21611 54-55 0.4275497971762121 21611 56-57 0.31407522576869695 21611 58-59 0.6469433904282482 21611 60-61 -0.04909953739421269 21611 62-63 0.12367121382126811 21611 64-65 -0.39419428958021996 21611 66-67 0.8816641395394953 21611 68-69 -0.19373094693592918 21611 70-71 -0.06604354981393357 21611 72-73 0.28938838745205686 21611 74-75 -0.18303921253180278 21611 76 0.08682633909258897 21612 1 0.7267850972799508 21612 2 0.2567242948884889 21612 3 0.2645990890943608 21612 4 -0.043687932338126956 21612 5 0.3275170578068298 21612 6 0.7582736065156794 21612 7 0.9488191021336974 21612 8 -0.09730601468181987 21612 9 -0.18923923944244336 21612 10-11 0.1645059903907935 21612 12-13 0.07333536695646359 21612 14-15 0.12413219863900338 21612 16-17 -0.6457158052275602 21612 18-19 -0.09669744238495781 21612 20-21 0.21191483161481983 21612 22-23 0.2839609213378864 21612 24-25 0.49156766139137886 21612 26-27 0.47180550906525554 21612 28-29 0.14316794478202866 21612 30-31 0.30031943275558604 21612 32-33 -0.22217477181316525 21612 34-35 0.6378797713502422 21612 36-37 0.3835066004897243 21612 38-39 -0.04513767330398366 21612 40-41 0.2737421294541633 21612 42-43 -0.06995796578977576 21612 44-45 0.30543559025045397 21612 46-47 -0.08048042103619935 21612 48-49 -0.12288738195103122 21612 50-51 -0.548857280129873 21612 52-53 0.36724745284808336 21612 54-55 -0.09647043038383174 21612 56-57 -0.12646913356795864 21612 58-59 0.25834370276557195 21612 60-61 0.355488846631971 21612 62-63 -0.31579483808621944 21612 64-65 0.33827391894212866 21612 66-67 -0.03794618246540438 21612 68-69 0.4717334689189059 21612 70-71 0.28132017443683566 21612 72-73 -0.03774777674813379 21612 74-75 0.3868276722752171 21612 76 -0.6333342915313409 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 8.0 11 16.0 12 43.0 13 79.0 14 171.0 15 303.0 16 572.0 17 933.0 18 1204.0 19 1188.0 20 1207.0 21 1290.0 22 1424.0 23 1667.0 24 2020.0 25 2335.0 26 2552.0 27 2801.0 28 3264.0 29 3584.0 30 4292.0 31 5305.0 32 6568.0 33 9471.0 34 15535.0 35 28239.0 36 81110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 75.71805103255993 6.430147702067377 3.9744667881996376 13.877334477173061 2 68.65634577070905 7.820251607113629 15.536090212833203 7.987312409344118 3 67.49350942544305 8.060729201941529 16.25747827068518 8.18828310193024 4 66.71144348807675 11.516861859743193 12.305347819952024 9.466346832228023 5 66.30620664743962 11.475271050507672 16.931838063900756 5.286684238151946 6 71.1759559757302 16.850571468886695 7.057993509242275 4.915479046140821 7 70.22107586113478 11.00186816722072 7.183695584691188 11.593360386953307 8 70.49497685969071 16.046393498137487 6.936448809120669 6.522180833051134 9 69.08133490611296 7.6656074861300025 6.544719806299773 16.70833780145727 10-11 70.28445648493059 11.009425443052264 11.172818602551079 7.53329946946608 12-13 69.68410834118782 8.478053515896173 11.219882493043837 10.617955649872163 14-15 72.65379839711028 10.419347556157579 7.884637092222599 9.042216954509538 16-17 72.20107292322193 9.468081804046179 7.864623164643765 10.466222108088125 18-19 68.9881615893666 9.209973049625376 10.905165723638724 10.896699637369304 20-21 72.14804780651625 8.872426589905318 8.691811652485573 10.287713951092861 22-23 72.382779980528 8.698057033201168 7.940906717839455 10.978256268431377 24-25 70.21949677725226 11.557303954215534 7.936369075167346 10.286830193364866 26-27 72.44091377291134 11.524318832808907 8.164411395352118 7.8703559989276295 28-29 69.87427861889913 11.93605282837832 8.682818077916215 9.506850474806338 30-31 69.79396251760251 11.674234449828278 10.811253185382938 7.720549847186281 32-33 69.8377264773946 13.881018231077496 8.820906474008016 7.460348817519896 34-35 74.6400492163204 9.388633964905152 8.940776737386907 7.030540081387539 36-37 73.10909429553497 8.7830248847198 11.221829018439188 6.8860518013060386 38-39 72.84041202201213 8.67532101030055 11.700578524058134 6.783688443629181 40-41 72.56495568028852 10.964648645292742 9.2116142759985 7.258781398420237 42-43 70.28601261446855 11.03836548094425 11.34596661539981 7.3296552891873965 44-45 69.78346817775194 8.666352484344971 11.636504223118363 9.913675114784724 46-47 72.13331263845578 8.279832371491866 9.46705986934007 10.11979512071228 48-49 72.62668051338203 8.177651852938853 11.742710720292587 7.452956913386538 50-51 74.8284921703254 8.32352683875301 9.472368261386116 7.375612729535469 52-53 69.9650352610503 8.59528667417322 11.561504358598814 9.87817370617767 54-55 71.87743399293366 8.590795696982696 9.274571335041596 10.257198975042048 56-57 71.64590611754214 8.784336744196548 8.889315325179622 10.680441813081686 58-59 69.75789951941121 11.035481167297387 11.413066483800236 7.793552829491163 60-61 69.07985709367362 9.231906715807177 11.769735691024332 9.918500499494861 62-63 69.2381933371901 11.305047198430952 11.407204639415276 8.049554824963666 64-65 69.21090654595943 11.639142557202362 11.71477271444536 7.435178182392847 66-67 69.20018286694737 14.326552958042193 8.638205646299202 7.835058528711239 68-69 69.2867967230972 14.862611080853034 8.168270031239505 7.682322164810262 70-71 69.47044630384784 14.592146293970737 8.185576611025667 7.751830791155761 72-73 70.01899246792023 14.17549266123138 8.034688769045381 7.7708261018030145 74-75 72.15759648706921 12.29553997764909 7.861198595730751 7.68566493955095 76 75.54790461408211 8.997319034852547 7.818258783688443 7.636517567376886 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 2.5 8 5.0 9 3.0 10 1.0 11 1.0 12 1.0 13 2.0 14 9.0 15 14.0 16 14.5 17 16.0 18 27.0 19 41.5 20 48.5 21 52.0 22 69.0 23 127.5 24 209.0 25 249.0 26 307.5 27 372.0 28 449.0 29 520.0 30 567.0 31 687.0 32 879.0 33 998.0 34 1156.0 35 1437.0 36 1700.5 37 1841.0 38 2075.5 39 2475.5 40 2885.0 41 3325.0 42 3521.0 43 4688.5 44 7705.5 45 6802.0 46 4049.0 47 3964.5 48 3527.0 49 3030.0 50 2886.0 51 2698.5 52 2330.0 53 1999.0 54 1849.0 55 1732.5 56 1450.5 57 1117.5 58 950.0 59 853.5 60 730.0 61 642.0 62 581.0 63 521.0 64 432.5 65 396.0 66 355.5 67 323.0 68 306.5 69 278.0 70 245.0 71 224.0 72 227.0 73 217.0 74 189.5 75 175.0 76 179.0 77 200.5 78 189.0 79 160.0 80 166.5 81 178.0 82 187.0 83 191.0 84 198.5 85 215.0 86 237.5 87 251.0 88 253.0 89 278.5 90 326.0 91 381.0 92 412.0 93 463.0 94 573.5 95 723.5 96 814.0 97 1079.0 98 2021.5 99 52677.5 100 102656.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 5.643946021300253E-4 4 0.0033863676127801516 5 0.0 6 0.0033863676127801516 7 0.0011287892042600505 8 5.643946021300253E-4 9 0.0 10-11 5.643946021300253E-4 12-13 0.0 14-15 5.643946021300253E-4 16-17 8.465919031950379E-4 18-19 0.0019753811074550882 20-21 0.004797354118105214 22-23 0.0019753811074550882 24-25 0.0016931838063900758 26-27 0.0019753811074550882 28-29 0.0019753811074550882 30-31 0.0025397757095851135 32-33 0.006208340623430277 34-35 0.002257578408520101 36-37 0.002257578408520101 38-39 0.0033863676127801516 40-41 0.0014109865053250632 42-43 0.0019753811074550882 44-45 8.465919031950379E-4 46-47 0.0019753811074550882 48-49 0.0016931838063900758 50-51 0.0014109865053250632 52-53 0.0014109865053250632 54-55 0.0016931838063900758 56-57 0.0011287892042600505 58-59 0.0014109865053250632 60-61 0.0011287892042600505 62-63 0.0019753811074550882 64-65 0.0016931838063900758 66-67 0.0016931838063900758 68-69 8.465919031950379E-4 70-71 0.0019753811074550882 72-73 0.0031041703117151384 74-75 0.0039507622149101765 76 0.0033863676127801516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 177181.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.701429611527196 #Duplication Level Percentage of deduplicated Percentage of total 1 72.55377736579514 15.019669151884232 2 9.321410071157883 3.8593302893651127 3 6.480547452220617 4.02469790778921 4 4.187682325036124 3.4676404354868753 5 2.8081463507729216 2.90663220096963 6 1.6903405218244774 2.0995479199236935 7 0.9842144006107038 1.4262251595825737 8 0.5670819815153084 0.939152617944362 9 0.35987894980779195 0.67050078733047 >10 0.9760353335696175 2.893086730518509 >50 0.040895335205430906 0.580762045591796 >100 0.021810845442896482 0.9566488506103927 >500 0.0027263556803620603 0.33920115588014516 >1k 0.0 0.0 >5k 0.0027263556803620603 3.8158719050011003 >10k+ 0.0027263556803620603 57.0010328421219 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 100995 57.0010328421219 No Hit CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG 6761 3.8158719050011003 No Hit TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT 601 0.33920115588014516 No Hit ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC 415 0.23422375988396046 Illumina Single End PCR Primer 1 (95% over 22bp) CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG 382 0.21559873801366963 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 215 0.12134483945795542 No Hit TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG 196 0.11062134201748496 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003386367612780151 0.0 2 0.0 0.0 0.0 0.003386367612780151 0.0 3 0.0 0.0 0.0 0.003386367612780151 0.0 4 0.0 0.0 0.0 0.003386367612780151 0.0 5 0.0 0.0 0.0 0.006772735225560302 0.0 6 0.0 0.0 0.0 0.006772735225560302 0.0 7 0.0 0.0 0.0 0.006772735225560302 0.0 8 0.0 0.0 0.0 0.006772735225560302 0.0 9 0.0 0.0 0.0 0.006772735225560302 0.0 10 0.0 0.0 0.0 0.008465919031950379 0.0 11 0.0 0.0 0.0 0.008465919031950379 0.0 12 0.0 0.0 0.0 0.009030313634080404 0.0 13 0.0 0.0 0.0 0.011287892042600504 0.0 14 0.0 0.0 0.0 0.011287892042600504 0.0 15 0.0 0.0 0.0 0.013545470451120605 0.0 16 0.0 0.0 0.0 0.013545470451120605 0.0 17 0.0 0.0 0.0 0.015238654257510682 0.0 18 0.0 0.0 0.0 0.023140178687331033 0.0 19 0.0 0.0 0.0 0.02709094090224121 0.0 20 0.0 0.0 0.0 0.02821973010650126 0.0 21 0.0 0.0 0.0 0.03217049232141144 0.0 22 0.0 0.0 0.0 0.039507622149101765 0.0 23 0.0 0.0 0.0 0.04120080595549184 0.0 24 0.0 0.0 0.0 0.05418188180448242 0.0 25 0.0 0.0 0.0 0.05700385481513255 0.0 26 0.0 0.0 0.0 0.06095461703004273 0.0 27 0.0 0.0 0.0 0.07054932526625315 0.0 28 0.0 0.0 0.0 0.07675766588968343 0.0 29 0.0 0.0 0.0 0.09368950395358419 0.0 30 0.0 0.0 0.0 0.1094925528132249 0.0 31 0.0 0.0 0.0 0.1230380232643455 0.0 32 0.0 0.0 0.0 0.14053425593037627 0.0 33 0.0 0.0 0.0 0.16028806700492718 0.0 34 0.0 0.0 0.0 0.1834282456922582 0.0 35 0.0 0.0 0.0 0.21672752721792968 0.0 36 0.0 0.0 0.0 0.2974359553225233 0.0 37 0.0 0.0 0.0 0.4295042922209492 0.0 38 0.0 0.0 0.0 0.5801976509896659 0.0 39 0.0 0.0 0.0 0.7359705611775529 0.0 40 0.0 0.0 0.0 0.9515692991912226 0.0 41 0.0 0.0 0.0 1.1649104587963721 0.0 42 0.0 0.0 0.0 1.3810735914121717 0.0 43 0.0 0.0 0.0 1.634486767768553 0.0 44 0.0 0.0 0.0 1.901445414576055 0.0 45 0.0 0.0 0.0 2.192108634673018 0.0 46 0.0 0.0 0.0 2.45737409767413 0.0 47 0.0 0.0 0.0 2.701756960396431 0.0 48 0.0 0.0 0.0 2.9224352498292707 0.0 49 0.0 0.0 0.0 3.1871363182282524 0.0 50 0.0 0.0 0.0 3.3846744289737614 0.0 51 0.0 0.0 0.0 3.5720534368809296 0.0 52 0.0 0.0 0.0 3.7893453587009893 0.0 53 0.0 0.0 0.0 4.043887324261631 0.0 54 0.0 0.0 0.0 4.296736106015882 0.0 55 0.0 0.0 0.0 4.523622736072152 0.0 56 0.0 0.0 0.0 4.782679858449834 0.0 57 0.0 0.0 0.0 4.981911153001732 0.0 58 0.0 0.0 0.0 5.209362177660132 0.0 59 0.0 0.0 0.0 5.411979839824812 0.0 60 0.0 0.0 0.0 5.581862615065949 0.0 61 0.0 0.0 0.0 5.755131757919867 0.0 62 0.0 0.0 0.0 5.927836506171655 0.0 63 0.0 0.0 0.0 6.136662508959764 0.0 64 0.0 0.0 0.0 6.339280171124443 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTACA 15 0.0022220393 69.998024 36 TATGCGC 15 0.0022220393 69.998024 3 TCGTGCT 15 0.0022220393 69.998024 47 TACTGTA 15 0.0022220393 69.998024 5 GGGCCCC 15 0.0022220393 69.998024 69 TGTGCGC 40 1.0604708E-8 61.248272 3 CTTTATA 40 1.0604708E-8 61.248272 2 TGCGGGT 35 2.962097E-7 59.99831 5 GCGCGTC 55 3.6379788E-11 57.271107 6 CGCGTCT 55 3.6379788E-11 57.271107 7 GGGCGCT 25 2.3858332E-4 55.99842 13 GATTGGC 25 2.3858332E-4 55.99842 10 TATACGC 25 2.3858332E-4 55.99842 3 TTATATC 20 0.006935408 52.51334 30 CGGCGTG 20 0.0069431574 52.498516 53 CGTGCTC 20 0.0069431574 52.498516 48 CTTTTCT 20 0.0069431574 52.498516 37 ATGTGAC 20 0.0069431574 52.498516 40 TCGGCGT 20 0.0069431574 52.498516 52 TGTGACA 20 0.0069431574 52.498516 41 >>END_MODULE