FastQCFastQC Report
Wed 25 May 2016
SRR1780144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177181
Sequences flagged as poor quality0
Sequence length76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT4068122.960136809251555No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT3278818.505370214639267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101825.746665838887917No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG55003.1041703117151385No Hit
CAAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT27181.5340245285894085No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC20921.1807135076560127No Hit
CACTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC18391.0379216733171164No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC10960.6185764839345077No Hit
CTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT9870.5570574723023348No Hit
ATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT9360.5282733475937036Illumina PCR Primer Index 3 (95% over 21bp)
AAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT5170.291792009301223Illumina PCR Primer Index 3 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC4110.23196618147544035No Hit
CATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGACGT4000.22575784085201012No Hit
CATAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT3480.1964093215412488No Hit
CAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC3190.18004187807947805No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2810.15859488319853707No Hit
ACTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2380.134325915306946No Hit
TCATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2380.134325915306946No Hit
CATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2360.13319712610268597No Hit
CATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCAGT1970.11118573661961498No Hit
CTTTACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC1930.10892815821109487No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG1810.10215542298553457No Hit
CATTACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC1810.10215542298553457No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC1810.10215542298553457No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTG150.00221983670.01552632
TACGGGG150.00221983670.01552625
TTACGGG150.00221983670.01552624
AACTCTC150.00221983670.01552632
GACTCTA207.901457E-570.01552626
TTTAGAC150.002222323669.9957717
TGGAGTC150.002222323669.995775
TCTTGTC150.002222323669.9957762
GCGACAT150.002222323669.9957738
GTATTCT150.002222323669.9957749
GAAGATC150.002222323669.9957758
GCAGTAT150.002222323669.9957746
CCAGCGT150.002222323669.9957759
TCCTCAG150.002222323669.9957716
TATGCAT150.002222323669.9957753
TCCAGCG150.002222323669.9957758
CCTTTCG150.002222323669.9957768
CAAATCG150.002222323669.9957736
AAATTAG150.002222323669.9957735
CTCGCAA150.002222323669.9957766
Produced by FastQC (version 0.11.5)