FastQCFastQC Report
Wed 25 May 2016
SRR1780143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127096
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9635775.81434506200037No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG65965.189777805753131No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT6150.48388619626109397No Hit
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG3980.3131491156291307No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC3520.2769560017624473Illumina Single End PCR Primer 1 (95% over 22bp)
TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG2180.171523887455152No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2070.16286901240007554No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1600.12588909171020332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT150.002220339670.0000152
ACGCTGA150.002220339670.0000115
GGCAATA150.002220339670.0000148
ACACCTG150.002220339670.0000133
TTGGGCG150.002220339670.000012
AGGTCTC150.002220339670.0000140
GTGTGCG150.002220339670.000012
ATACACC150.002220339670.0000131
CGAACAA308.337445E-658.33333625
GAACAAA308.337445E-658.33333626
TGCGGGC308.337445E-658.33333619
AAAAAGG6850.057.7372370
CGCCGTA9550.057.5392753
TCGCCGT9450.057.4074152
GTCGCCG9700.057.3711351
GGTCGCC9700.057.3711350
CATTAAA9350.057.27272461
GCCGTAT9350.057.27272454
ATTAAAA9500.057.1052662
ATCTCGG9450.057.0370442
Produced by FastQC (version 0.11.5)