##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780143_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 127096 Sequences flagged as poor quality 0 Sequence length 76 %GC 90 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.01424907156795 32.0 27.0 32.0 22.0 32.0 2 29.536405551708945 32.0 32.0 32.0 22.0 32.0 3 29.608272486939008 32.0 32.0 32.0 22.0 32.0 4 29.132262226978032 32.0 32.0 32.0 13.0 32.0 5 29.751628690124 32.0 32.0 32.0 22.0 32.0 6 33.97634071882671 37.0 37.0 37.0 22.0 37.0 7 33.55118178384843 37.0 37.0 37.0 22.0 37.0 8 33.976726254170075 37.0 37.0 37.0 27.0 37.0 9 34.04004846730031 37.0 37.0 37.0 27.0 37.0 10-11 33.91837272612828 37.0 37.0 37.0 22.0 37.0 12-13 33.6024973248568 37.0 37.0 37.0 22.0 37.0 14-15 34.244468748032986 37.0 37.0 37.0 27.0 37.0 16-17 34.42106360546359 37.0 37.0 37.0 27.0 37.0 18-19 33.94785437779316 37.0 37.0 37.0 24.5 37.0 20-21 34.41856942783408 37.0 37.0 37.0 27.0 37.0 22-23 34.529347894504944 37.0 37.0 37.0 29.5 37.0 24-25 34.10386636872914 37.0 37.0 37.0 27.0 37.0 26-27 34.5382191414364 37.0 37.0 37.0 29.5 37.0 28-29 34.32093456914458 37.0 37.0 37.0 27.0 37.0 30-31 34.26692028073268 37.0 37.0 37.0 27.0 37.0 32-33 34.15264052369862 37.0 37.0 37.0 24.5 37.0 34-35 34.2375881223642 37.0 37.0 37.0 27.0 37.0 36-37 34.94296830742117 37.0 37.0 37.0 32.0 37.0 38-39 35.13294674891421 37.0 37.0 37.0 32.0 37.0 40-41 34.70572638005917 37.0 37.0 37.0 29.5 37.0 42-43 34.54563086171083 37.0 37.0 37.0 29.5 37.0 44-45 34.43198841820356 37.0 37.0 37.0 27.0 37.0 46-47 34.65451705797193 37.0 37.0 37.0 29.5 37.0 48-49 35.128442279851456 37.0 37.0 37.0 32.0 37.0 50-51 35.35429124441367 37.0 37.0 37.0 32.0 37.0 52-53 34.617159470006925 37.0 37.0 37.0 29.5 37.0 54-55 34.81072575061371 37.0 37.0 37.0 29.5 37.0 56-57 34.706973468873926 37.0 37.0 37.0 29.5 37.0 58-59 34.401822244602506 37.0 37.0 37.0 27.0 37.0 60-61 34.22231226789199 37.0 37.0 37.0 24.5 37.0 62-63 33.906275571221755 37.0 37.0 37.0 22.0 37.0 64-65 33.69773242273557 37.0 37.0 37.0 22.0 37.0 66-67 33.771365739283695 37.0 37.0 37.0 22.0 37.0 68-69 33.91069349153396 37.0 37.0 37.0 22.0 37.0 70-71 33.996034493611134 37.0 37.0 37.0 24.5 37.0 72-73 34.16158258324416 37.0 37.0 37.0 27.0 37.0 74-75 33.933699723044 37.0 37.0 37.0 24.5 37.0 76 34.9263234090766 37.0 37.0 37.0 32.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -1.5037757378736494 11101 2 -0.41394492667384597 11101 3 -0.9749772637461902 11101 4 -1.7192454762093732 11101 5 -1.7431072106289598 11101 6 -0.9899293599081389 11101 7 -2.4159158309070676 11101 8 -2.078450729668944 11101 9 -0.6473640242772163 11101 10-11 -1.870285185079318 11101 12-13 -2.114275673007395 11101 14-15 -0.9175514530002999 11101 16-17 -1.443902092629763 11101 18-19 -1.0659052157759064 11101 20-21 -0.957875057076528 11101 22-23 -1.9710206490913365 11101 24-25 -1.5941756117186117 11101 26-27 -0.3496127645953564 11101 28-29 -0.6203137199552913 11101 30-31 -0.8687611432100866 11101 32-33 -1.1631429255970787 11101 34-35 -1.5417635043020184 11101 36-37 -0.4911598948212301 11101 38-39 -1.4739915189957102 11101 40-41 -1.5756263066062814 11101 42-43 -1.0369763895476964 11101 44-45 -1.0065252346901161 11101 46-47 -1.5810581592257478 11101 48-49 -1.3289371700392465 11101 50-51 -0.3660107919677742 11101 52-53 -2.4384782790106954 11101 54-55 -1.3833029741880694 11101 56-57 -1.1868964451204178 11101 58-59 -2.147734978445797 11101 60-61 -1.3176786265632643 11101 62-63 -2.164448424422247 11101 64-65 -0.66231957152295 11101 66-67 -1.724804667825282 11101 68-69 -1.945550301183303 11101 70-71 -2.0815292606581295 11101 72-73 -2.3470195034769468 11101 74-75 -2.8354521042777705 11101 76 -0.6555863275843095 11102 1 -0.5554796156644848 11102 2 -2.058872462905729 11102 3 -1.0905275966878278 11102 4 -0.04787845701626381 11102 5 -0.6804358436097644 11102 6 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-0.5743405199824778 21611 44-45 -0.08895277092200615 21611 46-47 0.46332589874526064 21611 48-49 0.2856461632940821 21611 50-51 0.45871746890178855 21611 52-53 0.15640396736612416 21611 54-55 -0.2680402930286476 21611 56-57 0.7164097142998784 21611 58-59 0.018478789670147933 21611 60-61 -0.3858398584473264 21611 62-63 0.13718201036036248 21611 64-65 -0.5930260932620826 21611 66-67 -0.5160184359412199 21611 68-69 -1.1661118953862015 21611 70-71 -0.13066875341175432 21611 72-73 -0.10018979333202083 21611 74-75 0.11721999717150311 21611 76 0.00881584632873711 21612 1 0.5905813389071106 21612 2 0.9517658349666114 21612 3 0.3574310002069403 21612 4 1.2318800248698771 21612 5 0.695067319127439 21612 6 0.14944776004252702 21612 7 0.7381082208968195 21612 8 1.1275313856563685 21612 9 0.7041623402001207 21612 10-11 0.7782655395583618 21612 12-13 0.4467018169710144 21612 14-15 0.5852237551403121 21612 16-17 0.9618179197044938 21612 18-19 0.5912024006286529 21612 20-21 0.6761058248229475 21612 22-23 0.5658279478867669 21612 24-25 0.291732498056291 21612 26-27 0.2797427704031037 21612 28-29 0.3547864958942313 21612 30-31 -0.5099884019211558 21612 32-33 -0.3657331198616518 21612 34-35 0.868504765818237 21612 36-37 0.6152230839021726 21612 38-39 0.4846116262401736 21612 40-41 0.4496589231192729 21612 42-43 0.4785185225707167 21612 44-45 0.23861815106381812 21612 46-47 0.7962159696672515 21612 48-49 0.2898571562018901 21612 50-51 0.10831853273158032 21612 52-53 0.6250431517632862 21612 54-55 0.9565829339217018 21612 56-57 0.22028826039916538 21612 58-59 0.5009699244219163 21612 60-61 0.4781893968718265 21612 62-63 0.07468201036036248 21612 64-65 0.1496600769506813 21612 66-67 0.36230603214389134 21612 68-69 0.9205459060322454 21612 70-71 0.36766901254569007 21612 72-73 0.5024254548949258 21612 74-75 -0.5041230879348788 21612 76 0.731555208030862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 0.0 5 0.0 6 1.0 7 0.0 8 0.0 9 0.0 10 1.0 11 8.0 12 28.0 13 64.0 14 149.0 15 292.0 16 555.0 17 804.0 18 1050.0 19 1016.0 20 1022.0 21 996.0 22 1011.0 23 1148.0 24 1237.0 25 1381.0 26 1570.0 27 1685.0 28 1884.0 29 2161.0 30 2499.0 31 3033.0 32 3842.0 33 5313.0 34 8610.0 35 16002.0 36 69733.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 85.13328507584818 2.364354503682256 2.148769434128533 10.353590986341034 2 83.09624221061244 2.158997922829987 11.594385346509725 3.1503745200478375 3 83.10804431296029 2.461131742934475 11.893371939321458 2.537452004783786 4 83.90680693361449 8.405001140914777 4.671455885939996 3.0167360395307297 5 83.32835022345314 4.1669289356077295 11.024737206521056 1.4799836344180777 6 85.53252761778869 10.140685487678217 2.4084914864822333 1.918295408050861 7 84.19292655100516 3.8837090365474647 2.466658798536528 9.456705613910854 8 85.25422128503313 9.99024344186193 2.004815333532661 2.750719939572285 9 83.59586454333731 2.4933908226852144 1.76008686347328 12.150657770504186 10-11 84.93567803611471 6.157598646681616 6.024627247334672 2.8820960698689957 12-13 84.27075915952304 2.701926519243555 6.546703437206173 6.480610884027239 14-15 89.0652231213782 5.16820161375984 2.5543984987548636 3.2121767661071097 16-17 88.46523045934505 2.891167167608227 2.1751616913465623 6.468440681700159 18-19 84.55945992729791 2.3667521676869088 5.822068705052954 7.251719199962232 20-21 88.57030450861593 2.2377842473837437 2.8967660712880634 6.2951451727122505 22-23 88.4911954930996 2.196799219475349 1.901348608116827 7.410656679308228 24-25 85.60918708174819 6.026760247538191 1.8954847491767741 6.468567921536845 26-27 88.82773434887463 5.989385600138483 2.049310929527152 3.133569121459736 28-29 85.46722059610362 6.619614137789948 2.382132628332232 5.531032637774211 30-31 85.25757539991659 6.395124676407872 5.321467632918663 3.0258322907568713 32-33 85.53072625698323 9.17932174049886 2.9585333228420807 2.3314186796758203 34-35 91.75128351397605 3.366445698998761 2.688199539705333 2.1940712473198656 36-37 89.7569861084337 2.333751667105983 5.9272256602526525 1.982036564207677 38-39 89.28654326573165 2.3089482070145757 6.4358636426224995 1.9686448846312723 40-41 88.90650510454984 5.754470169364833 3.098924012038476 2.240100714046856 42-43 85.95567725361062 5.872980277833513 5.81436063277743 2.3569818357784413 44-45 85.4508688485249 2.3313544752485376 6.481055286068918 5.73672139015764 46-47 88.56432469009037 1.9422708478403696 3.3306187963979275 6.162785665671336 48-49 89.7453046194519 1.9513269810296396 5.9110257842682135 2.3923426152502496 50-51 92.56211027401302 1.9839880402069359 3.2381926549560363 2.2157090308240064 52-53 86.0905006569992 2.210585948872086 5.853980943088919 5.8449324510397895 54-55 88.77221833174654 2.0949555830074513 2.7657347884586634 6.367091296787342 56-57 88.64610954183158 2.27864634558945 2.4718119802034733 6.603432132375504 58-59 85.92111164441228 5.552320778025194 5.82063246020568 2.705935117356854 60-61 84.96297986513812 3.033605312645071 6.479113719874423 5.524301102342379 62-63 85.05177351839612 6.250983539483208 5.74701790828691 2.950225033833758 64-65 85.0307458071217 6.3891478906763295 6.430063616084601 2.1500426861173727 66-67 84.90091706303777 10.543274280925798 2.3318029278348895 2.2240057282015573 68-69 84.94700647567491 11.001959226066361 1.9305064874774767 2.1205278107812515 70-71 85.07044026641803 10.856574097023058 1.8596545063280183 2.213331130230893 72-73 85.83967393442494 10.192303152858976 1.8061861186079264 2.161836794108159 74-75 88.99279641512477 7.157554321954214 1.757422918494447 2.0922263444265656 76 93.37561274991934 2.8153055684509525 1.7066511397345208 2.1024305418951776 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 1.5 12 2.0 13 2.5 14 1.5 15 3.0 16 8.5 17 11.0 18 13.5 19 16.0 20 21.5 21 27.0 22 24.5 23 24.5 24 34.0 25 41.0 26 53.5 27 83.0 28 119.0 29 138.0 30 157.5 31 193.0 32 210.0 33 211.0 34 257.5 35 288.5 36 283.0 37 293.0 38 321.5 39 389.5 40 471.0 41 705.5 42 898.0 43 1778.0 44 4442.5 45 3652.0 46 1077.0 47 905.5 48 621.0 49 485.0 50 462.0 51 417.5 52 385.5 53 390.0 54 382.0 55 382.5 56 392.5 57 405.5 58 409.0 59 393.5 60 341.0 61 277.5 62 251.0 63 240.0 64 230.0 65 199.0 66 178.5 67 190.0 68 188.5 69 158.0 70 137.5 71 146.0 72 136.0 73 118.5 74 116.0 75 121.0 76 132.5 77 133.5 78 118.5 79 114.0 80 126.5 81 143.5 82 147.0 83 146.0 84 149.0 85 174.0 86 198.0 87 200.0 88 231.5 89 291.0 90 312.0 91 357.0 92 409.0 93 445.0 94 558.5 95 726.5 96 817.0 97 1066.5 98 2016.0 99 50235.5 100 97755.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.003934034115943854 5 0.0 6 0.0031472272927550826 7 7.868068231887706E-4 8 0.0015736136463775413 9 0.0 10-11 7.868068231887706E-4 12-13 0.0011802102347831561 14-15 0.0011802102347831561 16-17 0.0015736136463775413 18-19 0.0015736136463775413 20-21 0.0047208409391326246 22-23 0.0015736136463775413 24-25 0.003540630704349468 26-27 0.003540630704349468 28-29 0.0031472272927550826 30-31 0.0055076477623213944 32-33 0.0047208409391326246 34-35 0.0027538238811606972 36-37 0.003540630704349468 38-39 0.0027538238811606972 40-41 0.0027538238811606972 42-43 0.004327437527538239 44-45 0.001967017057971927 46-47 0.001967017057971927 48-49 0.0023604204695663123 50-51 0.0027538238811606972 52-53 0.0023604204695663123 54-55 0.003934034115943854 56-57 0.0023604204695663123 58-59 0.003934034115943854 60-61 0.0023604204695663123 62-63 0.0031472272927550826 64-65 0.004327437527538239 66-67 0.004327437527538239 68-69 0.003934034115943854 70-71 0.001967017057971927 72-73 0.003934034115943854 74-75 0.0051142443507270095 76 0.003934034115943854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 127096.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.179738150689243 #Duplication Level Percentage of deduplicated Percentage of total 1 82.65887817580406 9.241046138352111 2 7.516362868604405 1.680619374331214 3 4.173411218241959 1.399729338452823 4 2.2450559504539376 1.0039655063888713 5 1.1541980434935604 0.645181595014792 6 0.7389682595538039 0.49568829860892555 7 0.29558730382152154 0.2313212060174986 8 0.16186923780702372 0.1447724554667338 9 0.07741572242944612 0.0778938754956883 >10 0.8023083960869872 1.5932838169572607 >50 0.11260468717010347 0.8591930509221376 >100 0.0422267576887888 1.13929627997734 >500 0.007037792948131467 0.48388619626109397 >1k 0.0 0.0 >5k 0.007037792948131467 5.189777805753131 >10k+ 0.007037792948131467 75.81434506200037 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 96357 75.81434506200037 No Hit CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG 6596 5.189777805753131 No Hit TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT 615 0.48388619626109397 No Hit CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG 398 0.3131491156291307 No Hit ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC 352 0.2769560017624473 Illumina Single End PCR Primer 1 (95% over 22bp) TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG 218 0.171523887455152 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 207 0.16286901240007554 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 160 0.12588909171020332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.003934034115943854 0.0 3 0.0 0.0 0.0 0.003934034115943854 0.0 4 0.0 0.0 0.0 0.007081261408698936 0.0 5 0.0 0.0 0.0 0.011015295524642789 0.0 6 0.0 0.0 0.0 0.011015295524642789 0.0 7 0.0 0.0 0.0 0.011015295524642789 0.0 8 0.0 0.0 0.0 0.011015295524642789 0.0 9 0.0 0.0 0.0 0.011015295524642789 0.0 10 0.0 0.0 0.0 0.011015295524642789 0.0 11 0.0 0.0 0.0 0.013375715994209102 0.0 12 0.0 0.0 0.0 0.013375715994209102 0.0 13 0.0 0.0 0.0 0.013375715994209102 0.0 14 0.0 0.0 0.0 0.013375715994209102 0.0 15 0.0 0.0 0.0 0.013375715994209102 0.0 16 0.0 0.0 0.0 0.015736136463775415 0.0 17 0.0 0.0 0.0 0.015736136463775415 0.0 18 0.0 0.0 0.0 0.016522943286964183 0.0 19 0.0 0.0 0.0 0.02360420469566312 0.0015736136463775413 20 0.0 0.0 0.0 0.024391011518851893 0.0015736136463775413 21 0.0 0.0 0.0 0.02517781834204066 0.0015736136463775413 22 0.0 0.0 0.0 0.03540630704349468 0.0015736136463775413 23 0.0 0.0 0.0 0.03934034115943853 0.0015736136463775413 24 0.0 0.0 0.0 0.04327437527538239 0.0015736136463775413 25 0.0 0.0 0.0 0.04720840939132624 0.0015736136463775413 26 0.0 0.0 0.0 0.04720840939132624 0.0015736136463775413 27 0.0 0.0 0.0 0.048782023037703785 0.0015736136463775413 28 0.0 0.0 0.0 0.0590105117391578 0.0015736136463775413 29 0.0 0.0 0.0 0.06530496632466797 0.0015736136463775413 30 0.0 0.0 0.0 0.07710706867249953 0.0015736136463775413 31 0.0 0.0 0.0 0.0912695914898974 0.0015736136463775413 32 0.0 0.0 0.0 0.09756404607540757 0.0015736136463775413 33 0.0 0.0 0.0 0.1156606030087493 0.0015736136463775413 34 0.0 0.0 0.0 0.12982312582614716 0.0015736136463775413 35 0.0 0.0 0.0 0.14713287593630012 0.0015736136463775413 36 0.0 0.0 0.0 0.20771700132183546 0.0015736136463775413 37 0.0 0.0 0.0 0.26830112670737083 0.0015736136463775413 38 0.0 0.0 0.0 0.3280984452697174 0.0015736136463775413 39 0.0 0.0 0.0 0.3949770252407629 0.0015736136463775413 40 0.0 0.0 0.0 0.5004091395480582 0.0015736136463775413 41 0.0 0.0 0.0 0.6011204129162208 0.0015736136463775413 42 0.0 0.0 0.0 0.7270095046264241 0.0015736136463775413 43 0.0 0.0 0.0 0.8497513690438724 0.0015736136463775413 44 0.0 0.0 0.0 0.9740668471076981 0.0015736136463775413 45 0.0 0.0 0.0 1.0747781204758609 0.0015736136463775413 46 0.0 0.0 0.0 1.162900484673003 0.0015736136463775413 47 0.0 0.0 0.0 1.262824951217977 0.0015736136463775413 48 0.0 0.0 0.0 1.3257694970730787 0.0015736136463775413 49 0.0 0.0 0.0 1.4115314408006545 0.0015736136463775413 50 0.0 0.0 0.0 1.5083086800528733 0.0015736136463775413 51 0.0 0.0 0.0 1.56810599861522 0.0015736136463775413 52 0.0 0.0 0.0 1.623182476238434 0.0015736136463775413 53 0.0 0.0 0.0 1.7105180336123875 0.0015736136463775413 54 0.0 0.0 0.0 1.7923459432240196 0.0015736136463775413 55 0.0 0.0 0.0 1.8568641027254988 0.0015736136463775413 56 0.0 0.0 0.0 1.938692012337131 0.0015736136463775413 57 0.0 0.0 0.0 2.0197331151255744 0.0015736136463775413 58 0.0 0.0 0.0 2.079530433687921 0.0015736136463775413 59 0.0 0.0 0.0 2.140901365896645 0.0015736136463775413 60 0.0 0.0 0.0 2.206206332221313 0.0015736136463775413 61 0.0 0.0 0.0 2.256561968905394 0.0015736136463775413 62 0.0 0.0 0.0 2.308491219235853 0.0015736136463775413 63 0.0 0.0 0.0 2.3659281173286333 0.0015736136463775413 64 0.0 0.0 0.0 2.414710140366337 0.0015736136463775413 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCCGT 15 0.0022203396 70.00001 52 ACGCTGA 15 0.0022203396 70.00001 15 GGCAATA 15 0.0022203396 70.00001 48 ACACCTG 15 0.0022203396 70.00001 33 TTGGGCG 15 0.0022203396 70.00001 2 AGGTCTC 15 0.0022203396 70.00001 40 GTGTGCG 15 0.0022203396 70.00001 2 ATACACC 15 0.0022203396 70.00001 31 CGAACAA 30 8.337445E-6 58.333336 25 GAACAAA 30 8.337445E-6 58.333336 26 TGCGGGC 30 8.337445E-6 58.333336 19 AAAAAGG 685 0.0 57.73723 70 CGCCGTA 955 0.0 57.53927 53 TCGCCGT 945 0.0 57.40741 52 GTCGCCG 970 0.0 57.37113 51 GGTCGCC 970 0.0 57.37113 50 CATTAAA 935 0.0 57.272724 61 GCCGTAT 935 0.0 57.272724 54 ATTAAAA 950 0.0 57.10526 62 ATCTCGG 945 0.0 57.03704 42 >>END_MODULE