SRR1780143_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780143_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127096
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT	39038	30.715364763643233	No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT	30141	23.715144457732737	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	10087	7.93652042550513	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG	5178	4.074085730471454	No Hit
CAAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT	2482	1.9528545351545288	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC	1575	1.2392207465223137	No Hit
CACTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC	1507	1.1857178825454775	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC	965	0.7592685843771637	No Hit
CTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT	900	0.7081261408698936	No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT	720	0.566500912695915	TruSeq Adapter, Index 3 (95% over 23bp)
AAACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT	497	0.39104299112481905	RNA PCR Primer, Index 3 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT	397	0.31236230880594196	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT	349	0.274595581292881	No Hit
CTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC	344	0.27066154717693713	TruSeq Adapter, Index 3 (95% over 21bp)
CAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC	344	0.27066154717693713	TruSeq Adapter, Index 3 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT	327	0.257285831182728	No Hit
CATAAACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT	261	0.20535658085226913	No Hit
ACTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG	246	0.19355447850443758	No Hit
TCATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG	239	0.1880468307421162	No Hit
CATATACAAATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT	237	0.18647321709573866	No Hit
CATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG	227	0.17860514886385095	No Hit
CATTACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC	207	0.16286901240007554	No Hit
CCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG	206	0.16208220557688677	No Hit
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATATCGTATGCCGT	199	0.15657455781456536	No Hit
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGA	185	0.14555926228992258	No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT	172	0.13533077358846857	No Hit
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTAAGCCGT	168	0.1321835462957135	No Hit
CATATACACATATCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT	168	0.1321835462957135	No Hit
CTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG	167	0.13139673947252473	No Hit
CTTTACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC	164	0.1290363190029584	RNA PCR Primer, Index 3 (95% over 21bp)
CATATACACATCTCCGAGCCAACGAGACCACTAGGCATCTCGTATGCCGT	151	0.11880783030150438	No Hit
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCAGT	146	0.11487379618556053	No Hit
CATAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG	146	0.11487379618556053	No Hit
CATATACACATCTCCGAGACCACGAGACCACTAGGCATCTCGTATGCCGT	140	0.11015295524642789	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC	137	0.10779253477686157	No Hit
TATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCT	134	0.10543211430729528	RNA PCR Primer, Index 3 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCACGTATGCCGT	128	0.10071127336816264	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTACA	35	3.6670826E-9	70.02717	1
CTTATAT	15	0.0022169235	70.02717	1
CGTCAGA	15	0.002220389	69.99961	20
AATCTTG	15	0.002220389	69.99961	54
ACTCGAA	20	7.901768E-5	69.99961	38
AACCGAG	15	0.002220389	69.99961	12
AGCCATC	20	7.901768E-5	69.99961	33
CCAAAGA	15	0.002220389	69.99961	19
TAACGTA	20	7.901768E-5	69.99961	38
TATGCCC	15	0.002220389	69.99961	43
GGCAAAT	20	7.901768E-5	69.99961	34
CACAAGG	30	1.016997E-7	69.99961	29
ATCTACG	75	0.0	69.99961	10
TATAAGC	15	0.002220389	69.99961	31
CACGAAT	20	7.901768E-5	69.99961	39
CTGATAG	15	0.002220389	69.99961	54
TCTACGA	75	0.0	69.99961	11
GAATGCA	15	0.002220389	69.99961	42
TGATAGA	15	0.002220389	69.99961	55
ATAACGT	20	7.901768E-5	69.99961	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content