FastQCFastQC Report
Wed 25 May 2016
SRR1780142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280646
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8363129.799462668272486No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG58982.101579926312864No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT5150.18350519872009577No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC3600.12827547871696016Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG3150.11224104387734014No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATGT150.00222330569.9987519
TTGTCAA308.364548E-658.3322912
CCGAGTT252.388123E-455.99900444
TTGTCCG200.0069470852.4990622
CGCGATG200.0069470852.4990618
CGCTGGC407.464823E-752.4990616
ATCAAGG200.0069470852.4990648
TCGCTAC200.0069470852.4990613
TCAACGT407.464823E-752.4990615
GCCGTAT9500.051.5780354
CGTATCA9600.051.40533456
CCGTATC9650.050.77629555
TGGTCGC9650.050.77629549
GTCGCCG9850.050.45595651
TCGCCGT9800.050.35624352
GTATCAT9800.050.35624357
GATCTCG10050.050.1483641
CGCCGTA9850.050.1006353
CTCGGTG10000.050.04910744
CGGTGGT10000.050.04910746
Produced by FastQC (version 0.11.5)