FastQCFastQC Report
Wed 25 May 2016
SRR1780142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280646
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT3372212.015849148037029No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT253519.033087947093492No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88363.1484503609529444No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG46451.6551099962229996No Hit
CAAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT20780.7404345688162312No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC15200.5416075768049429No Hit
CACTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC15000.5344811613206674No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC8090.2882635063389466No Hit
CTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT7320.26082680672448566No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT5730.20417180362449489TruSeq Adapter, Index 11 (95% over 21bp)
AAACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT3540.12613755407167748RNA PCR Primer, Index 11 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT3080.1097467984578437Illumina PCR Primer Index 6 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACTCGCACACATCTCGTATGCCGT3030.10796519458677478No Hit
CATATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGACGT2850.10155142065092679No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAA207.9119985E-570.01105529
AATCTCG207.9189886E-569.9985735
GCCGTAA401.3460522E-1069.9985746
ATATACG150.002224899469.986110
CATATAC40550.066.828871
ATATACA40650.066.492212
AGGGGGG70350.066.1180670
CAAATAC5750.062.107831
TGCATGA4150.059.86763455
GTCTTCA4550.058.44993249
CCGTCTT80550.058.03937547
GCCGTCT80850.058.00747346
CGTCTTC80200.057.94360748
TGCCGTC85350.057.9425845
ATGCCGT86500.057.77916744
TATGCCG86700.057.72662443
CGTATGC86400.057.72451841
GTATGCC86950.057.60089542
CTCGTAT87350.057.4573339
TCGTATG87300.057.45014640
Produced by FastQC (version 0.11.5)