FastQCFastQC Report
Wed 25 May 2016
SRR1780141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218800
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8479038.752285191956126No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG61912.8295246800731264No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT5760.263254113345521No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC3860.17641681901279707Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG3680.16819012797074953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTC252.833307E-670.06
GCTTCTA252.3867462E-455.9999961
TGCGCGT252.3867462E-455.9999965
TATCTAG252.3867462E-455.9999966
GCCGCAC200.006944553552.50000419
TAGTTCC200.006944553552.50000427
AATTGCA200.006944553552.50000417
TTCGGCT200.006944553552.50000436
TCGCCGT9500.052.31579252
GCCGTAT9400.052.1276654
CGTATCA9600.051.4062556
GTCGCCG9700.051.23711451
ATCTCGG9750.050.97435842
CGCCGTA9700.050.8762953
TCGGTGG9900.050.55555745
AGATCTC9800.050.3571440
GTAGATC9800.050.3571438
CGGTGGT9800.050.3571446
TCTCGGT9750.050.2564143
TGGTCGC9750.050.2564149
Produced by FastQC (version 0.11.5)