FastQCFastQC Report
Wed 25 May 2016
SRR1780141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218800
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT3305815.108775137111518No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT2916613.329981718464351No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92884.244972577696526No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG47382.1654478976234004No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC20180.9223034734917732No Hit
CAAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT20000.9140767824497258No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC18550.8478062157221207No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC8840.4040219378427788No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT7780.3555758683729433No Hit
ATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT7680.35100548446069474RNA PCR Primer, Index 30 (95% over 21bp)
AAACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT3620.16544789762340037RNA PCR Primer, Index 30 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC3220.14716636197440586No Hit
CATATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGACGT2760.12614259597806216No Hit
CAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC2510.11471663619744057No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG2470.11288848263254113No Hit
CATAAACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT2320.10603290676416818No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAA150.002220822370.0132539
GTCGCCA150.002222836769.9972524
GTCTAAC150.002222836769.9972521
ATCTTAC252.8339673E-669.9972558
AACTCGG252.8339673E-669.9972548
CATATAC37850.067.238621
ATATACA38100.067.073072
AGGGGGG72900.066.3157170
CTTTATA1200.061.2615972
CGTCTAC401.0621079E-861.247648
AAGCCGT401.0621079E-861.247644
AGCCGTC352.9655712E-759.99764645
CAAATAC3500.059.0111731
GCCGTCA1250.058.797746
CTCGGCA308.359473E-658.33104350
AAGGGGG82950.058.30995669
TGCCGTC85250.057.9273545
ATGCCGT85350.057.90048644
CGTCTTC84700.057.84897648
CCGTCTT84100.057.84553547
Produced by FastQC (version 0.11.5)