FastQCFastQC Report
Wed 25 May 2016
SRR1780140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161019
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11359270.54571199672088No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG81825.0813879107434525No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT7630.4738571224513878No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC4670.29002788490799225Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG4360.27077549854365013No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3270.2030816239077376No Hit
TCTTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG2480.15401909091473678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGGG207.908307E-569.999384
CGTCTGG401.3460522E-1069.999389
TGTGCGC700.069.999383
GTTGTGC401.3460522E-1069.999381
CGGGTCA150.002221499869.99937456
AAAAGTG150.002221499869.99937432
TTATGCG252.8300528E-669.9993742
GTCTACC150.002221499869.99937439
TATGCGC252.8300528E-669.9993743
GCGCATC150.002221499869.9993746
AAAGTGT150.002221499869.99937433
CTTATGC301.0187614E-769.9993741
TCATGAA150.002221499869.99937460
GGTGCCG150.002221499869.99937417
CATAACG150.002221499869.99937429
TGTAGGT252.8300528E-669.99937437
GAGCAAT150.002221499869.99937411
TCCCCTT150.002221499869.99937437
GCGCTGG500.069.99937415
CGCCGAA150.002221499869.99937453
Produced by FastQC (version 0.11.5)