FastQCFastQC Report
Wed 25 May 2016
SRR1780140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161019
Sequences flagged as poor quality0
Sequence length76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT4650428.881063725398864No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT3492921.6924710748421No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122727.6214608213937485No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG59743.710121165825151No Hit
CAAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT29911.8575447617982972No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC19311.1992373570820836No Hit
CACTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC18721.1625957185176905No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC10890.676317701637695No Hit
CTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT10340.6421602419590234No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTT9300.5775715909302629TruSeq Adapter, Index 6 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT5780.35896384898676553No Hit
AAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTT5410.3359851942938411RNA PCR Primer, Index 6 (95% over 21bp)
CAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC4390.2726386327079413No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT4310.2676702749364982No Hit
CTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC4260.2645650513293462No Hit
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGT3950.24531266496500412No Hit
CATAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT2860.1776187903290916No Hit
CATATACAAATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT2820.17513461144337003No Hit
CATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG2760.1714083431147877No Hit
TCATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG2750.17078729839335732No Hit
CATATACACATCTCCGAGCCCACGAGACACCACACCATCTCGTATGCCGT2570.1596084934076103No Hit
CATTACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC2520.1565032698004583No Hit
ACTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG2500.15526118035759756No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC2160.1341456598289643No Hit
CCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG2160.1341456598289643No Hit
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTAAGCCGT2050.12731416789323No Hit
TATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCT2020.12545103372893882No Hit
CTTTACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC2000.12420894428607804No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGT1780.11054596041460946No Hit
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGA1760.10930387097174868No Hit
CATATACACATCTCCGAGCCAACGAGACACCACAACATCTCGTATGCCGT1760.10930387097174868No Hit
CATAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1700.10557760264316633No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1690.10495655792173596No Hit
CATATACACATATCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT1640.10185133431458399No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACACCATCTCGTATGCCGT1640.10185133431458399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAGA455.456968E-1270.0158519
AGCGCAA150.002219427170.0158527
ACTCGCC150.002219427170.0158527
CGCAAGT150.002219427170.0158529
TTTTCCA150.002219427170.0158565
CTTAATG150.00222216469.994112
CCCCTAT150.00222216469.994156
TACTTCC150.00222216469.994151
CAAGTAT150.00222216469.994139
GAATGAC207.9112615E-569.994142
GGCACAT150.00222216469.994118
TAGGTAT150.00222216469.994157
GTAGGTC150.00222216469.994134
TATGCTA150.00222216469.994153
CAACACG150.00222216469.994136
TGGCACA150.00222216469.994117
ATATCTT150.00222216469.99418
ACCTATA150.00222216469.99412
TATCTTA150.00222216469.99419
CGCTACT150.00222216469.994148
Produced by FastQC (version 0.11.5)