FastQCFastQC Report
Wed 25 May 2016
SRR1780139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288717
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10630736.82048511171839No Hit
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG78182.7078419351822025No Hit
TCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGT6610.2289439139364845No Hit
CTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGG5260.18218532334431295No Hit
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC4320.14962748989494903Illumina Single End PCR Primer 1 (95% over 22bp)
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3150.10910337804840033No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTCG150.002223212370.0000132
TCCGTCC252.388029E-455.9999968
GTCATCG200.00694679352.58
TAGAGCA200.00694679352.59
ATTAACG200.00694679352.518
ATCCGAG200.00694679352.529
TTCGGCG200.00694679352.517
TGTCGTC200.00694679352.547
CTTACGT200.00694679352.564
GCCGTAT11950.050.3765754
ATCTCGG11950.050.08368342
GATCTCG11950.050.08368341
CGCCGTA11950.050.08368353
GCGCGTC352.083557E-550.06
CGTATCA12050.049.95850856
TGGTCGC12050.049.95850849
TCGCCGT12000.049.87552
CGGTGGT12150.049.83539246
AGATCTC12150.049.5473340
AAAAAGG9550.049.4764470
Produced by FastQC (version 0.11.5)