FastQCFastQC Report
Wed 25 May 2016
SRR1780139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288717
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT4498915.582386904823755No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT3428011.873218411108457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116974.051372104863932No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG60412.0923603390171No Hit
CAAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT26960.9337863721221819No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC23680.8201803149797207No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC23010.796974199648791No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC11830.40974379755954793No Hit
CTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT10010.3467062902426944No Hit
ATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCTT9460.3276564940755134Illumina PCR Primer Index 10 (95% over 22bp)
AAACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCTT5630.1950006407658711Illumina PCR Primer Index 10 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC4500.15586196864057192No Hit
CAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC3650.12642137456401945No Hit
CATAAACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT3180.11014245783933747No Hit
CATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGACGT3050.10563977874527652No Hit
CATATACAAATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT3040.10529341881496414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCG252.8358554E-669.9989610
CGTATGG150.002223343669.99895527
CGCTTCG150.002223343669.99895513
ATATACA51150.067.478912
CATATAC51200.067.413021
AGGGGGG93500.067.07921670
ATATCGT601.8189894E-1258.33246637
TAAGCCG308.36501E-658.33246643
CAAATAC5550.058.0272061
AAGGGGG107850.057.9592769
CCGTCTT110350.057.8831547
CGTCTTC111350.057.80336448
GCCGTCT110950.057.79094346
ATGCCGT111950.057.77493744
TATGCCG112100.057.72884443
TGCCGTC112000.057.71789645
CTGCTTG111400.057.5574954
TCGTATG114350.057.5417840
GTATGCC112350.057.5380942
CGTATGC112850.057.50025641
Produced by FastQC (version 0.11.5)