FastQCFastQC Report
Wed 25 May 2016
SRR1780138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69272
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1931027.875620741425106No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG698110.077664857373831No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT6300.9094583670169765No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC4570.6597182122646957No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG2990.4316318281556762No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG2340.33779882203487704No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG860.12414828502136505No Hit
CTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGG850.12270470031181428No Hit
CTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT800.11548677676406051No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG150.002216061870.04
TATGGGC207.876203E-570.03
GGCGCTG150.002216061870.014
TCTGGCG150.002216061870.011
AGTGTAG308.297577E-658.33333235
AGCCGAC252.3753263E-455.99999619
CGAGGAA252.3753263E-455.99999625
CAGTGTA252.3753263E-455.99999634
ACGAGGA252.3753263E-455.99999624
AAAAAGG5750.055.39130870
TTTATAC452.6542693E-854.4444473
GCAAGAG200.006924618552.569
AGAAGTT200.006924618552.550
TGGCGCT200.006924618552.513
TTCCAGG200.006924618552.51
GGCGGTG200.006924618552.514
GCGTCTG200.006924618552.58
AGGGACA200.006924618552.527
AGAGCTA200.006924618552.58
CGTATCA10750.052.41860256